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SEPARATION OF ENDOPHYTIC BACTERIA ISOLATES FROM THE
MEDICINAL PLANT INULA HELENIUM AND STUDY OF THEIR
ENZYME SYNTHESIS PROPERTIES.
Luiza Tagayeva*
Shermat Jabborov**
Kunduz Normurodova*
Department of Biotechnology and Microbiology, Faculty of Biology and Ecology,
National University of Uzbekistan, Tashkent, Uzbekistan
Scientific Research Institute of Livestock and Poultry, Tashkent, Uzbekistan.
e-mail: tagaevaluiza646@gmail.com
https://doi.org/10.5281/zenodo.16940223
Abstract.
A total of 14 bacterial isolates were isolated from the medicinal
plant black andes. The isolated active endophytic bacterial isolates were
identified using MALDI-TOF MS, 3 of which belonged to Bacillus
amyloliquyefaciyens, 5 to Bacillus subtilis, 2 to Klebsiyella oxytoca, and 4 to
Pseudarthrobacter oxydans. It has been established that the Bacillus subtilis-5
strain synthesizes more enzymes such as α-amylase, lipase, and protease.
Keywords:
Black elecampane, Inula helenium, medicinal plant,
gastrointestinal inflammation, endophytic bacteria, isolate, MALDI-TOF MS
method, identification, amylase, protease, lipase, Bacillus subtilis.
Log in.
Black andes (Inula helenium) is one of the widely used medicinal
plants in folk medicine, and its roots, leaves, petioles, and flowers are an
effective remedy against respiratory diseases, gastrointestinal diseases, and
inflammation. The essential oils and secondary metabolites contained in it are
used to combat cough, improve digestion, and fight microbes [1-3].
Nevertheless, it is recommended to use this medicinal plant with caution and
moderation.
Currently, the use of endophytic bacterial isolates isolated from medicinal
plants is used to improve plant nutrition, protect them from various
phytopathogens, and create biological preparations to increase agricultural crop
yields [4-5]. In particular, endophytic bacteria isolated from medicinal plants
can be used as probiotic preparations containing live bacteria that interfere with
protecting normal intestinal flora from pathogenic bacteria causing various
diseases, antibiotics, improper nutrition, chemical preparations, and
conditionally pathogenic microbes that multiply as a result of organism
weakening due to various infections can be used to prevent dysbacteriosis [3-5].
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Objective of the research:
To isolate endophytic bacterial isolates from
the medicinal plant Inula helenium and study their enzyme-synthesizing
properties.
Research and Methods.
The isolation of endophytic bacterial isolates from
the medicinal plant was carried out using classical microbiological methods, the
enzyme-forming property was determined by the GOST standard.
Received results and their analysis.
It is known that based on the
isolation of endophytic bacteria that synthesize secondary metabolites from
medicinal plants and their enzyme-synthesizing properties, they can be used as
probiotic preparations containing live bacteria. Accordingly, in this study,
endophytic bacteria in the internal tissues of black andiz, one of the medicinal
plants found in some regions of our republic, were studied. In particular, the
biodiversity of bacteria living in the internal tissues of the plant's petioles,
flowers, leaves, and roots was studied.
According to the obtained results, a total of 14 endophytic bacterial isolates
were isolated from the petioles, flowers, leaves, and roots of black andiz and
grown on a meat peptone (GPA) medium. Taking into account the biodiversity of
endophytic bacteria in the internal tissues of plants, it was established that they
are found differently in the roots, flowers, petioles, and leaves of the medicinal
plant black andiz. It was found that endophytic bacteria live in leaves - 35%,
roots - 22%, petioles - 28%, flowers - up to 15%.
The isolated active endophytic bacterial isolates were identified using
MALDI-TOF MS. According to the results obtained, it was established that 3
Bacillus amyloliquefaciens, 5 Bacillus subtilis, 2 Klebsiella oxytoca, 4
Pseudarthrobacter oxydans bacteria belong to them.
When screening isolated isolates based on enzyme-synthesizing properties,
it was found that the 5th isolate of Bacillus subtilis strains isolated from leaves
synthesizes more enzymes such as α-amylase, lipase, protease, which inhibit the
growth and development of pathogenic bacteria and break down cell walls,
compared to other strains.
Conclusion.
A total of 14 bacterial isolates were isolated from one of the
medicinal plants Black andiz. Isolated endophytic bacterial isolates were
identified using MALDI-TOF MS. As a result, it was established that 3 of the
isolated bacteria belong to the bacterial species Bacillus amyloliquefaciens, 5 to
Bacillus subtilis, 2 to Klebsiella oxytoca, and 4 to Pseudarthrobacter oxydans. It
was found that the Bacillus subtilis-5 strain synthesizes more enzymes such as
α-amylase, lipase, protease.
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List of used literature:
1.
Tojiboev Sh. “Xalq tabobatida qo‘llaniladigan o‘simliklar”. Samarqand,
2010.
2.
Soliev M., Tursunov M. “O‘zbekistondagi dorivor o‘simliklar va ularning
xalq tabobatidagi roli”. Toshkent: Fan va texnologiya, 2021.
3.
Normurodova K.T., Shoxiddinova М.N. Introduction of endophitic bacteria
of plants and study of their morphological and cultural properties // The
American Journal of Horticulture and Floriculture Research, 2021, 03 (12-02), P.
4-9. (Impact Factor – 5.731).
4.
Tagayeva L., Shoxiddinova M.N., Tojiyev B.B., Elova N.A., Normurodova Q.T.
Dorivor o‘simliklardan ajratilgan Bacillus amyloliquefaciens – UZMU 22
shtammning probiotik xususiyatlarini aniqlash // “O‘zMU xabarlari” jurnali. -
Toshkent, 2024. №3/2/1. – B. 116-118.
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Shokhiddinova M.N., Gafforova H.F., Axmedova M.O., Maxmudova M.M.,
Raximberdiyeva G.A., Normurodova K.T. Isolation of Endophytic Bacteria from
medicinal plants and screening for their enzyme production activity. Journal of
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