Study Of The Effect Of Polysaccharides On Hemostasis
Keywords:
Abstract
The effect of sulfated polysaccharides on the hemostatic system in conditions in vitro. Platelet-rich plasma was obtained by centrifugation at 200 g for 10 minutes. The remaining citrate blood was further centrifuged at 1500 g for 10 min to obtain platelet-poor plasma. The antithrombin activity of the compounds was evaluated in vitro by their effect on the recalcification time, thrombin, and prothrombin time of human blood plasma stabilized with a 3.8% sodium citrate solution in the ratio of 9:1. In studies conducted on the blood plasma of rats, it was found that the studied compounds, to varying degrees, lengthen the APTT, APTT, prothrombin time. At the same time, anticoagulant activity was established to block one of the factors II, V, X. Polysaccharide exhibit a combined anticoagulant effect in the body, due to which they are classified as anticoagulant and antithrombin agents.
Similar Articles
-
BIOCHEMICAL CHANGES OF CHRONIC GENERALIZED PERIODONTITIS IN CHRONIC VIRAL HEPATITIS B, C AND MIXED INFECTIONS
The American Journal of Medical Sciences and Pharmaceutical Research: No. 08 (2023) / Nazarova Nodira Sharipovna, Shukurov Sherzod Shukhratovich, -
Meaning Of Respiratory Mycoplasma Infection In Children With Bronchial Asthma
The American Journal of Medical Sciences and Pharmaceutical Research: No. 12 (2020) / Gulnoza Aloevna Toshmatova , Maftuna Shukhrat Qizi Shakarova , Arzi Ruslanovna Emirova, -
The Role Of The Nurse In The Prevention Of Bronchial Asthma In Children
The American Journal of Medical Sciences and Pharmaceutical Research: No. 05 (2021) / Jamilakhon Erkinovna Kamilova , -
The State Of The Oral Cavity With Dental Anomalies In Children With Bronchial Asthma
The American Journal of Medical Sciences and Pharmaceutical Research: No. 08 (2020) / Gafforov Sunnatullo Amrulloevich, Fazilbekova Gavkhar Anvarovna, -
Correction Of Cognitive Disorder In Patients With HIV - Associated Encephalopathy
The American Journal of Medical Sciences and Pharmaceutical Research: No. 07 (2020) / Zokirov M.M.,, Madjidova Y.N., -
Clinical And Epidemiological Assessment And Causes Of Mortality From COVID-19 Coronavirus Infection (Literature Review)
The American Journal of Medical Sciences and Pharmaceutical Research: No. 09 (2021) / Haitov Abdulaziz Haydar O'g'li, -
Features Of The Clinical And Laboratory Course Of Tuberculosis Lymphadenopathy In Patients Without And With HIV Infection
The American Journal of Medical Sciences and Pharmaceutical Research: No. 09 (2021) / Mirazim Alimovich Khakimov , Zarifa Abduraubovna Muminova , -
Clinical And Epidemiological Aspects Of A New Coronavirus Infection (Covid-19)
The American Journal of Medical Sciences and Pharmaceutical Research: No. 09 (2021) / Bayramgul Sadykovna Jumamuratova, -
Dangerous Symptoms Of Covid-19 Virus And Analysis Of Their Characteristics
The American Journal of Medical Sciences and Pharmaceutical Research: No. 10 (2021) / A.B. Tleubergenova , Z.U. Kunnazarova, -
CHARACTERIZATION OF MORPHOLOGICAL AND MORPHOMETRIC CHANGES IN THE BRONCHIAL WALL IN RABBITS DURING ACUTE EXPERIMENTAL PNEUMONIA
The American Journal of Medical Sciences and Pharmaceutical Research: No. 06 (2023) / Ismoilov Jasur Mardonovich,
References
Geerts W. Prevention of venous thromboembolism: American College of Chest Physicians evidence-based clinical practice guidelines (8th Edition)/ W. Geerts, D Bergqvist, G.F. Pineo J.A. Heit, C.M. Samama, M.R. Lassen, C.W. Colwell // Chest. - 2008. - 133 (6). - P.381S-453S.
Ageno W. Antithrombotic treatment of splanchnic vein thrombosis: results of an international registry / W Ageno, N Riva, S Schulman, et al. // Semin Thromb Hemost. - 2014.- Feb;40(l). - P.99-105.
Molteni M. Warfarin and atrial fibrillation: from ideal to real the warfarin affaire/ M. Molteni, C. Cimminiello // Thromb J. - 2014. - 12(1). - P.5.; Vo T., 2014.
Vo T. Current state of anticoagulants to treat deep venous thrombosis / T. Vo, S. Vazquez, M.T. Rondina // Curr Cardiol Rep. -2014. -16(3). -P.463.
Марри Р., Греннер Д., Мейес П., Родуэлл В. Биохимия человека / Л. Гинодман. — М: БИНОМ, 2009. — С. 331-336. — 451 с. — ISBN 978-5-9963-0016-7.
Nozim N. Khoshimov Nasirov E. Kabil Kamila A. Eshbakova. Research Influence Biological Active Agents in the Course of Regulation of Functional Activity of Platelets and System of a Haemostasis. European Journal of Medicine Vol. 8, Is. 2, pp. 88-93, 2015 DOI: 10.13187/ejm.2015.8.88
Verhaeghe R., DeMoerloose P., Eikenboom J.C. et al. Genetic and asquired risk factors of venous thromboembolism. In: Pulmonary Vascular Pathology: A Clinical Update. Eds. M. Demedts, M. Delcroix, R. Verhaeghe, G.M. Verleden. EurRespSoc 2004. –С. 9.
Nasirov K.E., Musaeva M.K., Khoshimov N.N., Raimova G.M., Turaev A.S., Muhitdinov B.I. Influence of some sulphated polysaccharides on the platelet aggregation in normal and in patients with ischemic heart disease. International Journal of Psychosocial Rehabilitation. Volume 24 - Issue 8. Pages: 6976-6985. DOI: 10.37200/IJPR/V24I8/PR280715
Nadjimova, X. K., Khoshimov, N. N., Muhitdinov, B. I., & Musayeva, M. K. (2006). FIBRINOLYTIC ACTIVITY OF POLYSACCHARIDES WITH ANTICOAGULANT PROPERTIES. O ‘ZBEKISTON BIOLOGIYA JURNALI, 8.
Wang Z.M., Li L., Zheng B.S., Normakhamatov N., GuoS.Y. Preparation and anticoagulation activity of sodium cellulose sulfate // Int. J. Biol. Macromol. 2007. Vol.41 (2). -P. 376-382.
Huynh R., Chaubet F., Jozefonvicz J. Anticoagulant properties of dextranmethylcarboxylate benzylamide sulfate (DMCBSu); a new generation of bioactive functionalized dextran // Carbohydr. Res. 2001. Vol. 332. -P. 75-83. 10.
Mahner C., Lechner M. D., Nordmeier E. Synthesis and characterization of dextran and pullulan sulphate // Carbohydr. Res. 2001. Vol. 331. -P. 203-208.
Scaglione F. 2013. New oral anticoagulants: comparative pharmacology with vitamin K antagonists. ClinPharmacokinet. - 522: 69-82. doi: 10.1007/s40262-012-0030-9.
Laboratory research methods in the clinic. 1987. Reference. Ed. V.V. Menshikov. M.: Medicine: 172.
Teien, A.N., Lie M. 1975. Heparin assay in plasma a comparison of five clotting methods. Thromb Res.-V.7. -N5: 777 - 788. doi.org/10.1016/0049-3848(75)90202-9.
Yin E.T. Wessler S., J. Butler. 1973. Plasma heparin: a unique, practical, submicrogram-sensitive assay. J Lab Clin Med. - V. 81. - N 2: 298-310.
Muhitdinov B, Heinze T, Normakhamatov N, Turaev A. 2017. Preparation of sodium cellulose sulfate oligomers by free-radical depolymerization. Carbohydr Polym [Internet]. 173:631–7. Available from: https://linkinghub.elsevier.com/retrieve/pii/S0144861717306707.
Muhitdinov B, Heinze T, Turaev A, Koschella A, Normakhamatov N. 2019. Homogenous synthesis of sodium cellulose sulfates with regulable low and high degree of substitutions with SO3/Py in N,N-dimethylacetamide/LiCl. Eur Polym J [Internet]. 119:181–8. Available from: https://linkinghub.elsevier.com/retrieve/pii/S0014305719312558
