Инфекционные заболевания
Биология ва тиббиёт муаммолари, 2014, №3 (79)
203
antibodies (Manufactured in Moscow, Russia). The
content of serum IgA, M, and G were measured by
Manchini method. Circulating immune complexes
(CIC) were revealed by method of precipitation with
3% and 4% solution of polyethilenglycole.
Results. In group of patients with PCR(-)
there was observed direct high dependence of CD3
from CD4, CD8; CD25 from CD20, CD16; CD95
from CD8, IgG from CD4; 3% and 4%CIC. The
direct moderate dependence: CD20 from the
lymphocytes; CD23, CD16; CD8 from the
lymphocytes, CD4, IgM, 3% and 4%CIC; CD16
from the lymphocytes, CD20; CD23 from CD20,
CD4, 3%CIC; CD95 from the lymphocytes, CD23,
4%CIC; IgG from the L and lymphocytes, CD3,
CD23; IgM from CD3, CD23, 3% and 4%CIC. The
inverse mean correlation: CD20 from the L and IgA
and M; CD16 from the L nd CD23; CD25 from the
L, CD3, CD4; IgA from the lymphocytes, CD20,
IgG; 3%CIC from the L and lymphocytes; 4%CIC
from the L and IgG.
In group of patients with HCV(+) there were
found direct high correlations between CD4 and
CD3, CD8, CD25. The direct moderate correlations:
CD8 and CD4, CD16; CD16 and CD20, IgА; CD25
and CD4, CD3, IgG, М; IgGand CD20; 3%CIC and
CD95; 4%CIC and CD20, CD23, 3%. Moderate
inverse correlations between: CD95 and the L,
3%CIC and CD16; 4%CIC and CD25, IgA.
IMMUNOGENETIC FACTORS IN ADULTS AND CHILDREN WITH CHRONIC VIRAL
HEPATITIS C INFECTION IN UZBEK POPULATION
E.U. Nazarov
Tashkent medical academy
Hepatitis C leads to severe liver damages
and one of the indications to liver transplantation
(according to WHO ~3% of worldwide population is
infected by HCV infection.) The current standard of
therapy of chronic hepatitis C (CHC) is pegylated
interferon-α (PEG-IFNα) in combined with ribavirin
(RBV). The treatment achieves a sustained viral
clearance in only approximately 50% of patients.
Recent whole genome association studies revealed
that single nucleotide polymorphisms (SNPs)
around IL-28B have been associated with response
to the standard therapy and could predict treatment
responses at approximately 80%. According to 4
independent GWAS approaches have revealed the
significant SNPs associated with response to PEG-
IFNα/RBV therapy for CHC. These significant
SNPs were found around IL-28B were rs12979860
and rs8099917 they showed the statistical
significance in each study (M. Mizokami at al.,
2011).
The purpose of research: To study
genotypes of HCV infection and polymorphisms of
gene IL28B circulation in adults and children with
CHC in Uzbek population.
Materials and methods: We studied 40
patients with CHC which were divided in to two
groups: children and comparative adults group each
included 20 patients. Diagnosis CHC was
established on the base of ELISA full marker
specter with following revelations of HCV RNA
and its genotype by PCR method. Gene IL-28B was
determined in all patients by PCR method.
Results: Research which we conducted
showed us variation of genotype of HCV infection.
In adult group we determined genotypes 1a/b (61%),
2 (11%) non revealed genotype (5%) and 3a/b
(22%) but there wasn’t 4 (0%). In children group we
exposed genotypes 1a/b (58%), 3a/b (32%), 4 (5%)
non revealed genotype (6%) but there wasn’t 2 (0%)
genotype. Also we established genotype distribution
of gene IL 28B in adults and children. We found
that most prevalent genotype in the adults group was
combination of C/C (rs12979860) and T/T
(rs8099917) (C/C T/T -45%) polymorphisms with
good prognosis in both lines (European and Asian
lines). Whereas in children group we found out that
most prevalent genotype was C/T (rs12979860) (not
as favorable as C/C European genotype) and T/T
(rs8099917) (C/T T/T- 35%). In addition, adults
revealed genotypes of gene IL28B T / T
(rs12979860), and T / T (rs8099917) (T/T T/T-5%),
that is not favorable by European line and favorable
by Asian line, as well as genotype C / C combined
with T / G genotype (C/C T/G-5%). In children we
detected T / T + G / G (5%) which has very low
therapy prognosis.
Conclusions:
1) The most prevalent genotypes of HCV in
region of Uzbekistan was 1a/b (61% in adults and
58% in children) and 3a/b (22% in adults and 32%
in children).
2) Interestingly that genotype 2 (11%) was
not exposed in children group but was determined in
adults group and conversely genotype 4 (5%) was
detected in the children group unlike adults group.
3) In Uzbek population we found both favorable
genotypes of gene IL 28B and very poor genotypes,
so there were differences among adults and children
in the distribution of polymorphisms of IL28B.
