The American Journal of Agriculture and Biomedical
Engineering
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TYPE
Original Research
PAGE NO.
6-9
10.37547/tajabe/Volume07Issue04-02
OPEN ACCESS
SUBMITED
13 February 2025
ACCEPTED
12 March 2024
PUBLISHED
09 April 2025
VOLUME
Vol.07 Issue04 2025
CITATION
Alamuratov Rayimjon Abdimurat o‘g‘li, Abdillayev Marat Ibodillayevich,
& Sattorov Shoximardon Xushmamatovich. (2025). Determination of the
insecticidal activity of the chemical preparation tranil 20% sc, received
for testing against cotton bollworm on cotton crops. The American
Journal of Agriculture and Biomedical Engineering, 7(04), 6
–
9.
https://doi.org/10.37547/tajabe/Volume07Issue04-02
COPYRIGHT
© 2025 Original content from this work may be used under the terms
of the creative commons attributes 4.0 License.
"Determination of the
insecticidal activity of the
chemical preparation
tranil 20% sc, received for
testing against cotton
bollworm on cotton
crops."
Alamuratov Rayimjon Abdimurat o‘g‘li
Institute of Plant Quarantine and Protection Research, Uzbekistan
Abdillayev Marat Ibodillayevich
Institute of Plant Quarantine and Protection Research, Uzbekistan
Sattorov Shoximardon Xushmamatovich
Institute of Plant Quarantine and Protection Research, Uzbekistan
Abstract:
To determine the insecticidal activity of
TRANIL 20% EC against the cotton bollworm
(Helicoverpa armigera Hbn), which causes significant
damage to cotton crops in our country, field trials were
conducted using a dosage rate of 0.2 liters per hectare.
The results of the trial showed that by the 7th day, the
effectiveness of the preparation reached 89.1%.
Keywords:
Cotton, egg, larva, polyphagous, pest,
formulation, insecticide, insecticidal activity.
Introduction:
Uzbekistan produces approximately 3.5
million tons of raw cotton annually (ranking sixth in the
world) and 1
–
1.2 million tons of cotton fiber. About 40%
of the produced cotton fiber is exported. To increase the
production and improve the quality of raw cotton in
Uzbekistan, it is essential to utilize all available
resources. In particular, protecting cotton crops from
pests plays a crucial role in enhancing both yield and
fiber quality [1].
The chemical method of protecting various agricultural
crops from their primary pests is an integral part of the
The American Journal of Agriculture and Biomedical
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The American Journal of Agriculture and Biomedical Engineering
Integrated Pest Management System (IPMS), as it
occupies a central position in this strategy. Gradual
changes in Uzbekistan's climate
—
such as increased
precipitation and humidity in the spring, changes in the
accumulation of effective temperatures, and the
redistribution of climatic parameters during autumn
and winter-have led to significant changes in plant
diseases and the development patterns of various
insects. Notably, there has been a marked increase in
fungal diseases that thrive in high-humidity
environments [11].
Globally, cotton bollworms cause annual agricultural
crop losses exceeding $2 million in crops such as
cotton, maize, vegetables, legumes, and others.
Meanwhile, control measures amount to nearly $1
million. In countries like China and India, nearly 50% of
all insecticides are used against cotton bollworm.
Among the chewing pests of cotton, the cotton
bollworm holds a particularly significant role [12].
Another critical reason for conducting such research is
the gradual decrease in the effectiveness of previously
recommended insecticides against target pests. This is
particularly evident in pyrethroids, where group and
cross-resistance has developed in pests such as aphids,
whiteflies, and cotton bollworms [6]. Subsequently,
new classes of chemical compounds have gained
popularity,
including
representatives
of
neonicotinoids-such as imidacloprid, acetamiprid,
thiamethoxam well as oxadiazines (e.g., Avaunt),
carbamates (e.g., Lannate), emamectins (e.g.,
Carrageenan), emamectin benzoates, and others,
which are now widely used against cotton bollworms.
The cotton bollworm was first described in the early
19th century. Its main scientific name is Helicoverpa
armigera Hbn. (Hübner, 1805). Synonyms include
Chloridea obsoleta, Chloridea armigera, Helicoverpa
obsoleta, Helicoverpa barbara, Helicoverpa conferta,
Helicoverpa pulverosa, Helicoverpa uniformis, and
Helicoverpa rama [10].
The cotton bollworm (Helicoverpa armigera Hübner) is
a polyphagous pest, damaging nearly 120 plant
species, with primary hosts including cotton, maize,
tomato, sunflower, soybean, chickpea, and others
[13;14]. The pest possesses high ecological plasticity,
allowing it to easily adapt to changing environmental
conditions and reach high population densities [15].
The cotton bollworm is widely distributed across
Central Asia, southern Kazakhstan, Transcaucasia, the
North Caucasus, the Lower Volga region, southern
Ukraine, and Far Eastern countries. In plantations, it
typically develops through 3
–
4 generations. The most
damaging stage is the larval stage, particularly the first
instar, which feeds on apical buds and leaf tissues.
From the second instar onward, larvae bore into fruiting
bodies [7].
Biological characteristics: In spring, moth emergence
begins when the soil temperature at a 10 cm depth
reaches 15
–
16 °C and the average daily air temperature
is 18
–
20 °C. Moth flight continues for over a month. The
flight periods of different generations usually overlap
and may last until late October. Depending on the air
temperature, adult moths live from 20 to 40 days. In
autumn, pests accumulate in underdeveloped cotton
fields, late-season maize, tomatoes, and other crops,
where most pupae overwinter [8].
METHODS
Field trials were conducted when the infestation level of
cotton bollworm reached 9
–
10 larvae per 100 cotton
plants. The tests of the TRANIL 20% SC formulation,
containing the active ingredient Chlorantraniliprole,
were carried out in the cotton field (And-36 variety) of
the “Yangi Diyor Fayzi” farm located in the Guliston
district, Sirdarya region. The treatment was applied
during the cotton budding and peak fruiting stages,
under early morning conditions with a temperature of
up to 23°C, wind speed of 1.0
–
1.3 m/s, and relative
humidity of 55
–
60%. The working solution was applied
at a rate of 300 liters per hectare using a battery-
powered sprayer (PT-16AC).
The distribution and damage caused by the pest were
determined based on generally accepted methods
[2;3;4;5]. The experiment included three variants:
experimental, control, and reference (standard), each
replicated three times. Pest population counts were
recorded before treatment, and on the 3rd, 7th, and
14th days after treatment. The effectiveness of the
tested formulation was evaluated in comparison with
the control variant. Field trials were conducted
following the methodology of Khodjaev Sh.T. (2023) [9],
and biological efficacy was calculated using the formula
developed by Püntener V. (1981) [16].
RESULTS
The experimental trials of TRANIL 20% SC at a dose of
0.2 litrs against the second and third generations of
cotton bollworm were conducted in July 2024. As a
reference sample, KORAGEN SC (Chlorantraniliprole 200
g/l) was used at the same dose (0.2 litr). The results
obtained from the experimental trial of TRANIL 20% SC
are presented in Table-1. As shown, the pre-treatment
pest counts in the experimental and standard variants
were 9.8 and 10.3 larvae per 100 plants, respectively
—
exceeding the established Economic Threshold of Pest
(ETP) for this cotton pest species. The control variant
had a count of 10.6 larvae. After treatment, the number
of pests per 100 plants in the experimental (TRANIL 20%
SC) variant was reduced to 2.2, 1.0, and 1.3 on the 3rd,
The American Journal of Agriculture and Biomedical
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The American Journal of Agriculture and Biomedical Engineering
7th, and 14th days, respectively. In the reference
variant (KORAGEN 20% SC), the corresponding counts
were 2.1, 1.1, and 1.4. Meanwhile, in the untreated
control variant, pest counts remained nearly
unchanged: 10.6 before treatment and 10.0 and 8.4 on
the 7th and 14th days, respectively.
Subsequent calculations of biological efficacy, adjusted
relative to the control variant, demonstrated a high
level of effectiveness for the test formulation. The
insecticidal activity of TRANIL 20% SC was recorded at
77.5%, 89.1%, and 83.2% on the 3rd, 7th, and 14th
days post-treatment, respectively. Similarly, the
reference product KORAGEN 20% SC showed a positive
efficacy of 79.6%, 88.6%, and 82.8% for the same
observation periods. These results clearly indicate that
chemical
formulations
containing
20%
Chlorantraniliprole exhibit high activity against cotton
bollworm.
CONCLUSION
When tested against cotton aphids, TRANIL 20% SC
demonstrated 89.1% insecticidal activity by the 7th day
when applied at a rate of 0.2 liters per hectare. This
formulation is convenient to use; when mixed with
water, it quickly forms a working solution. No phytotoxic
effects were observed on the cotton plants.
Table-1
Biological efficiency of insecticide TRANIL 20% c.k. against cotton bollworm
on cotton crops
(Syrdarya region, Gulistan district, farm "Yangi Diyor Fayzi", July 10, 2024).
№
Experie
nce
options
Active
ingredien
t
Cons
umpt
ion
rates
of
prep
arati
ons
Average number of cotton bollworm caterpillars per 100
plants, specimens
Biological efficiency,
% per days:
Before treatment (number of
caterpillars by age)
After the
treatments have
been carried out
Egg
s
I-II
III-
VI
V-VI
Total
3
7
14
3
7
14
1.
TRANI
L 20%
c.к.
Chlorant
raniliprol
e 200
gr/l.
0,2
10
4,2
3,3
2,3
9,8
2,2
1,0
1,3
77,5
89,1
85,2
2.
KORA
GEN,
с.к. 200
g/l
Chloratr
aniliprole
200 gr/l.
0,2
8
6,0
2,3
2,0
10,3
2,1
1,1
1,4
79,6
88,6
82,8
3.
Control
(withou
t
process
ing)
-
12
5,4
4,0
1,2
10,6
10,6
10,
0
8,4
-
-
-
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