Volume 03 Issue 06-2023
101
International Journal of Advance Scientific Research
(ISSN
–
2750-1396)
VOLUME
03
ISSUE
06
Pages:
101-106
SJIF
I
MPACT
FACTOR
(2021:
5.478
)
(2022:
5.636
)
(2023:
6.741
)
OCLC
–
1368736135
A
BSTRACT
The article describes the enzymatic activity of the irrigated meadow soils of the north-eastern part of the
Mirzachol oasis. Due to weak salinity of irrigated meadow soils, it was found that catalase, peroxidase, and
polyphenoloxidase activity of enzymes belonging to the class of oxidases was high. These indicators were
also observed in the activity of invertase and urease enzymes. It was observed that the activity of enzymes
was high in all studied cuttings in the spring, and decreased in the fall. The activity of enzymes depends on
the humus in the soil, the amount of nutrients, the mechanical composition, the amount of different
fertilizers used, and the hydrothermal regime of the area.
K
EYWORDS
Soil, microorganism, enzyme, oxidase, hydrolase, catalase, peroxidase, polyphenol oxidase, invertase,
urease.
I
NTRODUCTION
To preserve, restore and increase soil fertility in
the world, to study chemical and biological
processes that negatively affect the productivity
of agricultural crops due to the activity of
microorganisms and enzymes in the soil profile,
to identify microorganisms and enzymes involved
Journal
Website:
http://sciencebring.co
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Copyright:
Original
content from this work
may be used under the
terms of the creative
commons
attributes
4.0 licence.
Research Article
ENZYMATIC ACTIVITY OF IRRIGATED MEADOW SOILS OF
THE NORTHEASTERN PART OF THE MIRZACHUL OASIS
Submission Date:
June 07, 2023,
Accepted Date:
June 12, 2023,
Published Date:
June 17, 2023
Crossref doi:
https://doi.org/10.37547/ijasr-03-06-17
S.U.Nurillayev
2nd Stage Master’s Degree, Gulistan State University
, Uzbekistan
M.R.Obloqulov
Trainee Teacher, Gulistan State University, Uzbekistan
Volume 03 Issue 06-2023
102
International Journal of Advance Scientific Research
(ISSN
–
2750-1396)
VOLUME
03
ISSUE
06
Pages:
101-106
SJIF
I
MPACT
FACTOR
(2021:
5.478
)
(2022:
5.636
)
(2023:
6.741
)
OCLC
–
1368736135
in biological processes, to identify biological
processes in the soil It is important to carry out
scientific research aimed at regulating and
alternative, increasing the efficiency of the use of
degraded land and protecting the soil cover.
According to M.A. Galstyan, the activity of
enzymes is an effective indicator in determining
the biological condition of the soil, and the
amount of enzymes changes directly depending
on the soil composition, season, and climatic
conditions [2; 3].
F.Khaziev found that enzymes are biological
catalysts that speed up chemical reactions
hundreds and thousands of times in living
organisms [8;9].
L.A. Gafurova and her students studied the
activity of oxidoreductase (catalase, peroxidase,
polyphenoloxidase) and hydrolytic (phosphatase,
urease, invertase) enzymes, which are the most
important in the biodynamics of southern Aral
Bay soils, and found that the seasonal dynamics of
enzymatic activity is related to the level of
diversity and its activity is highest in spring.
found that it has an indicator, gradually decreases
in summer and slightly increases in autumn [1].
Catalase enzyme is closely related to nutrition
and respiration of plants and microorganisms, as
well as CO2 assimilation and soil humus synthesis
oxidation-reduction processes.
Agro-ecological factors, agro-technical and
amelioration measures, which cause the current
processes of soil formation, have a great influence
on enzymatic activity.
In section 1 of irrigated meadow soils, catalase
activity was 1.2-3.2 cm3 O2 in spring, and 0.7-2.0
cm3 O2 per 1 g of soil in autumn, catalase activity
in section 2 in spring was 1 ,5-3.4 cm3 O2, and in
autumn 0.8-2.4 cm3 O2 in 1 g of soil for 1 minute,
in section 3 catalase activity was 1.5-3.5 cm3 O2
in spring, and in autumn 1, 0-2.5 cm3 O2 was 1
minute in 1 g of soil, catalase activity in section 4
was 1.3-2.8 cm3 O2 in spring, and 0.6-2.4 cm3 O2
in 1 g of soil was 1 minute in autumn . In
conclusion, catalase activity was high in spring
and decreased in autumn (Table 1).
The conversion of organic matter into humus
takes place with the participation of enzymes
belonging to the group of phenoloxidases.
Salinity directly affects biochemical processes in
the soil. In our results, the activity of peroxidase
and polyphenoloxidase in irrigated meadow soils
changed mainly in proportion to the amount of
humus (Table 1).
Table 1
Activity of oxidase enzymes in irrigated meadow soils
Segme
nt №
Layer
depth,
cm
Catalase, 1
minute in O2 /1g
soil
Peroxidase, mg
purpurgallin /
100 g
Polyphenoloxidase,
mg purpurgallin /
100 g
Volume 03 Issue 06-2023
103
International Journal of Advance Scientific Research
(ISSN
–
2750-1396)
VOLUME
03
ISSUE
06
Pages:
101-106
SJIF
I
MPACT
FACTOR
(2021:
5.478
)
(2022:
5.636
)
(2023:
6.741
)
OCLC
–
1368736135
30 minutes
30 minutes
Spring
Autum
n
Spring
Autumn
Spring
Autu
mn
1
0-30
3,2
2,0
3,20
2,80
3,50
3,20
30-50
2,2
1,4
2,20
1,90
2,40
2,10
50-70
1,2
0,7
1,40
1,20
1,30
1,20
2
0-30
3,4
2,4
3,30
3,00
3,52
3,19
30-50
2,4
1,5
2,25
2,10
2,35
2,20
50-70
1,5
0,8
1,42
1,22
1,35
1,25
3
0-30
3,5
2,5
3,32
2,90
3,55
3,25
30-50
2,4
2,0
2,25
2,00
2,30
2,10
50-70
1,5
1,0
1,35
1,20
1,25
1,20
4
0-30
2,8
2,4
3,20
2,95
3,40
3,20
30-50
1,8
1,3
2,25
1,95
2,30
2,15
50-70
1,3
0,6
1,30
1,20
1,25
1,15
In the irrigated meadow soils, peroxidase activity
in section 1 was 1.40-3.20 mg in the spring period,
and 1.20-2.80 mg purpurgallin/100g of soil in 30
minutes in the autumn period, polyphenoloxidase
activity in the spring period was 1.30 -3.50 mg,
and in autumn 1.20-3.20 mg purpurgallin/100g
of soil in 30 minutes, peroxidase activity in
section 2 was 1.42-3.30 mg in spring and 1.22-3
,00 mg purpurgallin/100g of soil for 30 minutes,
polyphenoloxidase activity was 1.35-3.52 mg in
spring, and 1.25-3.19 mg of purpurgallin/100g of
soil for 30 minutes in section 3, peroxidase
activity in section 3 1.35-3.32 mg in spring and
1.20-2.90 mg purpurgallin/100g of soil in 30
minutes in spring, and 1.25-3.55 mg in spring and
1.20 in autumn -3.25 mg purpurgallin/100g of
soil for 30 minutes, peroxidase activity in section
4 was 1.30-3.20 mg in spring, and 1.20-2.95 mg
purpurgallin/100g of soil for 30 minutes in
autumn. polyphenoloxidase activity was 1.25-
3.40 mg in the spring period, and 1.15-3.20 mg
purpurgallin/100g soil in 30 minutes in the
autumn period (Table 1).
Due to weak salinization of the soils of the
experimental area, the activity of peroxidase and
polyphenoloxidase was high, the reason for this is
the active inclusion of humus substances in the
soil. Humus formation is a complex biochemical
process, the high activity of this process depends
on the activity of phenoloxidase enzymes and the
amount of different fertilizers used (Table 1).
Among the enzymes belonging to the group of
hydrolases, invertase and urease are among the
most common enzymes in the soil.
The main source of invertase in the soil is plant
root exudates. Silty and powdery fractions absorb
Volume 03 Issue 06-2023
104
International Journal of Advance Scientific Research
(ISSN
–
2750-1396)
VOLUME
03
ISSUE
06
Pages:
101-106
SJIF
I
MPACT
FACTOR
(2021:
5.478
)
(2022:
5.636
)
(2023:
6.741
)
OCLC
–
1368736135
the enzyme in such a way that all the structural
compounds in its molecule, which determine the
catalytic properties of the enzyme, are preserved.
In some sense, this situation is proved by the
higher activity of invertase in layers with more
humus [1;2;3;4;5;6;7;8;9;10].
In irrigated meadow soils, the fluctuation limits of
invertase enzyme activity in section 1 were 0.55 -
3.08 mg of glucose per 1g of soil in the spring
period, and 0.28 - 2.12 mg of glucose per 1g of soil
in the autumn period. , the fluctuating limits of
invertase enzyme activity in section 2 were 0.57 -
3.20 mg of glucose per 1g of soil in the spring
period, and 0.30 - 2.36 mg of glucose per 1g of soil
in the autumn period, in the interval of 1 hour in
the 3rd section of invertase enzyme The
fluctuating limits of the activity of invertase
enzyme were 0.56 - 3.11 mg of glucose per 1g of
soil in the spring period, 0.28 - 2.15 mg of glucose
per 1g of soil in the autumn period. .55 - 3.15 mg
of glucose was in 1 g of soil, in the autumn period
0.28 - 2.19 mg of glucose was in 1 g of soil within
1 hour (Table 2).
Urease hydrolyzes the breakdown of the bond
between nitrogen and carbon in the molecule of
organic matter. It is a single-component enzyme,
that is, it consists only of protein. The action of
urease is strictly directed, it hydrolyzes only urea.
Urea hydrolysis products serve as food for
microorganisms and plants. As a result of
hydrolysis, carbon monoxide and ammonia are
formed.
Table 2.
Activity of hydrolase enzymes in irrigated meadow soils
Segment
№
Layer depth, cm
Invertase, mg glucose in
1g soil 1 hour
Urease, mg NH3 in
1g of soil in 24 hours
Spring
Autumn
Spring
Autumn
1
0-30
3,08
2,12
1,91
1,36
30-50
1,91
1,45
1,46
1,14
50-70
0,55
0,28
0,84
0,56
2
0-30
3,20
2,36
2,09
1,59
30-50
2,12
1,63
1,52
1,21
50-70
0,57
0,30
0,85
0,58
3
0-30
3,11
2,15
1,98
1,42
30-50
1,93
1,47
1,51
1,19
50-70
0,56
0,28
0,84
0,56
4
0-30
3,15
2,19
2,05
1,54
30-50
1,93
1,48
1,51
1,21
Volume 03 Issue 06-2023
105
International Journal of Advance Scientific Research
(ISSN
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2750-1396)
VOLUME
03
ISSUE
06
Pages:
101-106
SJIF
I
MPACT
FACTOR
(2021:
5.478
)
(2022:
5.636
)
(2023:
6.741
)
OCLC
–
1368736135
50-70
0,55
0,28
0,85
0,58
In the irrigated grassland soils, the fluctuating
limits of urease enzyme activity in section 1 were
in the range of 0.84-1.91 mg NH3 in the spring
period, and in the range of 0.56-1.36 mg NH3 in
the autumn period. , the fluctuating limits of
urease enzyme activity in section 2 were in the
range of 0.85-2.09 mg NH3 in the spring period, in
the range of 0.58-1.59 mg NH3 in the autumn
period, urease enzyme in the 3rd section
fluctuating limits of urease activity were in the
range of 0.84-1.98 mg NH3 in the spring period, in
the range of 0.56-1.42 mg NH3 in the autumn
period. It was in the range of .85-2.05 mg NH3, in
the autumn period it was in the range of 0.58 -
1.54 mg NH3 (Table 2).
In our study, according to our observations, the
activity of hydrolases was the same as the activity
of oxidases. So, the activity of enzymes depends
on humus in the soil, the amount of nutrients,
mechanical composition, the amount of different
fertilizers used, and the hydrothermal regime of
the area.
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2750-1396)
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03
ISSUE
06
Pages:
101-106
SJIF
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FACTOR
(2021:
5.478
)
(2022:
5.636
)
(2023:
6.741
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