JOURNAL OF IQRO – ЖУРНАЛ ИҚРО – IQRO JURNALI – volume 15, issue 01, 2025
ISSN: 2181-4341, IMPACT FACTOR ( RESEARCH BIB ) – 7,245, SJIF – 5,431
ILMIY METODIK JURNAL
Ismatova Nafisa Nusratulla qizi
The user is a teacher at the "General Technical Sciences" department
of the International Asia University.
APPLICATION OF PULSED ELECTRIC FIELD (PEF) TREATMENT FOR OIL
EXTRACTION FROM FLAXSEED: IMPACT ON TISSUE DISINTEGRATION AND
YIELD
Abstract:
Pulsed Electric Field (PEF) technology is a promising method for enhancing oil
extraction processes from plant materials. This study investigates the effects of PEF treatment on
flaxseed tissue disintegration and oil yield. Different electric field strengths, pulse numbers, and
energy inputs were applied to flaxseed samples to evaluate their impact on tissue
permeabilization, oil release, and efficiency. The results demonstrate that PEF treatment
significantly enhances oil extraction efficiency, with optimal parameters leading to a higher yield
compared to traditional methods.
Keywords:
Pulsed Electric Field (PEF),Cell Disintegration,Electric Field Strength,Pulse Number
Introduction:
The extraction of oil from flaxseed has traditionally been performed through
mechanical pressing or solvent extraction. However, these methods can be inefficient,
particularly in terms of energy consumption and oil yield. The application of Pulsed Electric
Field (PEF) technology has gained attention as a potential method to enhance oil extraction
efficiency. PEF treatment involves applying short pulses of high-voltage electric fields to
biological tissues, leading to cell membrane permeabilization and enhanced release of
intracellular components, such as oils.
Materials and Methods:
Flaxseeds used in this study were obtained from a local supplier and
stored at a temperature of +4 to +8°C to maintain freshness. The seeds were ground to a fine
mash using a UMC 12 grinder. Ascorbic acid (1 g/kg) was added to prevent browning. The
flaxseed mash was divided into three equal portions for PEF treatment. Different electric field
strengths (ranging from 0.3 kV/cm to 4 kV/cm) and pulse numbers (from 10 to 150 pulses) were
applied to the samples in a PEF treatment chamber.
After PEF treatment, the flaxseed mash was manually pressed using a Hafico HP 2 press, and the
hydraulic pressure was increased to 250 bar three times until juice flow ceased. The control
samples were subjected to manual pressing without prior PEF treatment. The oil yield from each
sample was determined gravimetrically.
Results and Discussion:
1. Disintegration of Biological Tissue by PEF
The application of PEF treatment led to
significant tissue disintegration in flaxseed samples. The cell disintegration index (Zp) was
measured using impedance analysis after different pulse treatments. The data showed typical
sigmoid curves, with an increase in pulse number resulting in higher tissue disintegration. The
highest level of disintegration was achieved at an electric field strength of 4 kV/cm with 20
pulses, reaching a disintegration index of 0.6, as shown in
Figure 4.1
.At lower field strengths
(0.3 kV/cm), tissue permeabilization was still achieved, though a higher number of pulses
(approximately 150) was required. This suggests that PEF treatment can be effective even at
JOURNAL OF IQRO – ЖУРНАЛ ИҚРО – IQRO JURNALI – volume 15, issue 01, 2025
ISSN: 2181-4341, IMPACT FACTOR ( RESEARCH BIB ) – 7,245, SJIF – 5,431
ILMIY METODIK JURNAL
lower electric field strengths, offering potential for energy savings in large-scale oil extraction
processes.
2. Electric Field Strength and Specific Energy Input.
The impact of electric field strength and specific energy input on flaxseed tissue disintegration
was further analyzed. The relationship between cell disintegration index and total specific energy
input is shown in Figure 4.2. The results indicate that increasing the electric field strength
beyond a certain threshold (0.5 kV/cm) does not significantly improve energy efficiency. For
flaxseed, the minimum electric field strength required to achieve substantial tissue disintegration
was found to be around 0.5 kV/cm. However, at lower field strengths (0.3 kV/cm), a higher total
energy input was required to achieve a similar level of permeabilization.
3. Effect of Moderate Electric Field on Oil Yield.
In Figure 4.3, the impact of moderate electric fields (sinusoidal current at 60 V/cm) on oil yield
is shown. The treatment at 0.3 kV/cm resulted in cell disintegration but with a lower juice yield
compared to higher field strengths (1 kV/cm). The energy required to achieve a 50% cell
disintegration was ten times higher for moderate electric field (MEF) treatment than for PEF at
0.3 kV/cm. The maximum energy input of 32.4 kJ/kg resulted in a temperature increase of 8°C,
which could potentially lead to thermal effects and reduce extraction efficiency. Therefore, PEF
JOURNAL OF IQRO – ЖУРНАЛ ИҚРО – IQRO JURNALI – volume 15, issue 01, 2025
ISSN: 2181-4341, IMPACT FACTOR ( RESEARCH BIB ) – 7,245, SJIF – 5,431
ILMIY METODIK JURNAL
treatment with high-voltage pulses is more efficient for achieving significant tissue disintegration
and higher oil yield.
Comparison to Other Methods.
Comparing PEF treatment with traditional mechanical pressing
methods, PEF provided a higher oil yield with lower energy input. The results obtained for
flaxseed are consistent with findings for other plant materials such as potatoes, where PEF
treatment also showed enhanced tissue disintegration and higher oil recovery. The application of
PEF technology in oil extraction from flaxseed appears to be a promising alternative to
conventional methods, offering improved efficiency and potentially reducing energy
consumption.
Conclusion:
The application of Pulsed Electric Field (PEF) technology significantly enhances
oil extraction from flaxseed by promoting tissue disintegration and facilitating the release of
intracellular oil. The optimal parameters for PEF treatment in this study were found to be an
electric field strength of 4 kV/cm and 20 pulses, which resulted in the highest oil yield. Lower
field strengths (0.3 kV/cm) were also effective, though higher energy inputs were required. This
study provides valuable insights into the potential of PEF technology for large-scale oil
extraction from flaxseed, with implications for improved efficiency and reduced operational
costs in the oil extraction industry.This study investigated the process of cell disintegration in
samples treated with Pulsed Electric Field (PEF). The results showed that the effectiveness of
PEF treatment is directly related to the electric field strength and the number of pulses applied.
Higher field strength and pulse numbers led to more efficient cell disintegration. However, even
at lower electric field strengths, sufficient cell permeabilization was achieved with an adequate
energy input.Based on the findings, optimal processing parameters for PEF treatments can be
selected, offering a more energy-efficient approach for large-scale operations. The results also
indicate the potential for scaling up the PEF process for various agricultural products beyond ,
demonstrating the broader applicability of PEF technology in food processing. Further research
is recommended to improve energy efficiency and process control for industrial applications of
PEF.
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https://researchcitations.com/index.php/ibast/article/view/1149
JOURNAL OF IQRO – ЖУРНАЛ ИҚРО – IQRO JURNALI – volume 15, issue 01, 2025
ISSN: 2181-4341, IMPACT FACTOR ( RESEARCH BIB ) – 7,245, SJIF – 5,431
ILMIY METODIK JURNAL
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