American Journal Of Agriculture And Horticulture Innovations
41
https://theusajournals.com/index.php/ajahi
VOLUME
Vol.05 Issue07 2025
PAGE NO.
41-44
10.37547/ajahi/Volume05Issue07-08
Effect of The Zerox Immunostimulant On Cotton Yields
Atoeva Rukhsora
Bukhara State University, Muhammad Iqbol 11, 200118, Uzbekistan
Atoeva Dilsora
Bukhara State University, Muhammad Iqbol 11, 200118, Uzbekistan
Received:
31 May 2025;
Accepted:
27 June 2025;
Published:
29 July 2025
Abstract:
In order to obtain high-quality yields from agricultural crops, various immunostimulants and biologically
active substances are applied to the seeds before planting, as well as during different phenophases of the plants.
In this article, three different norms (1-2-3 l/t) of the Zerox immunostimulant were applied to the seeds of the
Bukhara-10 cotton variety before sowing and at different phenophases of the plant, and the effect on yield was
evaluated. As a result, the most positive indicator was observed in variant 10, with an average three-year yield of
46.0 q/ha. It was found that this yield was 7.6 q/ha higher than that of the control variant with the same number
of seedlings.
Keywords:
Zerox immunostimulant, different number of seedlings, Bukhara-10 cotton variety, yield.
Introduction:
In order to obtain high and quality yields
from crops in global agriculture, the demand for
stimulants with various effects is increasing year by
year.
Nowadays,
various
stimulants
and
immunostimulants are used on 50-80% of the areas,
where crops important for agriculture are grown [1].
Growth regulators, as universal agents, play an
important role not only in controlling plant growth and
development but also in accelerating physiological and
biochemical processes in plants. They enhance
resistance to drought and help achieve higher crop
yields under adverse conditions such as salinity [2].
Immunostimulants terminate the latent period in
plants, accelerate hypocotyl germination in seeds, and
increase the germination rate [3]. They accelerate stem
growth and branching in plants during the budding,
juvenil, virginal, and immature stages of the
pregenerative period, as well as during the young
generativ, mature generativ, and old generative stages
of the generative period [4-5].
Additionally, they enhance leaf area, dry mass, and net
photosynthetic productivity [6-7]. Furthermore,
immunostimulants improve valuable signs [8] and
production
in
cotton
[9].
One
of
these
immunostimulants, Zerox, contains 3000 mg/l of
colloidal silver and is used to enhance the immune
system in plants [10].
As a result of climate change and the negative impact
of anthropogenic factors, plants are being infected with
various phytoparasites. The most common bacterial
disease in cotton is cotton gummosis. For example, R.O.
Atoeva
and
others
studied
the
role
of
immunostimulants in increasing the resistance of
cotton to gummosis [11]. Furthermore, R.O. Atoeva
investigated
Zerox
Immunostimulant
increases
relatively the resistance of cotton to root rot [12].
METHODS
In order to determine the optimal rate of the
immunostimulant Zeroks that positively affects the
yield of cotton, different rates of the Zeroks
immunostimulant (1.0-2.0-3.0 l/t; 1.0-2.0-3.0 l/ha)
were applied to three different seedling of the
Bukhara-10 cotton variety (80,000-90,000; 100,000-
110,000; and 120,000-130,000 plants per hectare).
Also, the immunostimulant Fitovak was used at a rate
of 200 ml/t and ml/ha as the standard. The control
variant seeds was applied in drinking water, and during
the growing season, it was suspended with water.
American Journal Of Agriculture And Horticulture Innovations
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American Journal Of Agriculture And Horticulture Innovations (ISSN: 2771-2559)
Аll phеnological observаtions аnd analysis cаrried out
in the invеstigation in laborаtory and fiеld condition
were conducted on the bаsis of “Methods experiments
of field” which written Uzbekistаn Cotton Science
Research Institute [13] аnd Plаnt Insect Protection
Center, аs well as, methods given in severаl other
literature. Research dаtа wаs mаthemаtically
processed in the method of B. Dospexov [14].
In scientific research, the experimental system
consisted of 15 variants, 3 repetitions, each variant -
270 m2 area , the total area of calculation was 4050 m2.
In the experiment, hairy seedlings of the Bukhara-10
cotton variety were applied with the Zerox
immunostimulant at different norms (1-2-3 l/t of
seedlings before sowing) and at different times (2-4
true leaf, shading and flowering phases) together with
1-2-3 l/ha + 0.15 l of PAV. The experimental system is
presented in Table 1.
Table 1.
Experience system
№
Variants
and names
of
preparation
s
A number
of
different
seedlings
thousand
bushes/ha
Application norms and terms, l/t; l/ha
Applicati
on to
seeds
before
planting
2-4 true
leaves
Shading
Flowerin
g
1
Control
80-90
the seeds
swell in
water
The above ground parts of the
cotton plant were sprayed with
water
2
100-110
3
120-130
4
Fitovak
80-90
200
ml/t
200
ml/ha
200
ml/ha
200
ml/ha
5
100-110
6
120-130
7
Zerox
80-90
1 l/t
1
l/ha+PАV
0,15 l/ha
1
l/ha+PАV
0,15 л/га
1 l/ha
+PАV
0,15
8
100-110
9
120-130
10
Zerox
80-90
2 l/t
2
l/ha+PАV
0,15 l/ha
2 l/ha
+PАV
0,15 l/ha
2 l/ha
+PАV
0,15 l/ha
11
100-110
12
120-130
13
Zerox
80-90
3 l/t
3
l/ha+PАV
0,15 l/ha
3 l/ha +
PАV 0,15
l/ha
3 л/га
+PАV
0,15 l/ha
14
100-110
15
120-130
RESULTS AND DISCUSSION
Three different seedling thicknesses were left in the
Bukhara-10 cotton variety in moderately saline soil
conditions and Zeroks was applied to it at different
norms and periods, and the effect on cotton yield was
studied.
It was found that with increasing seedling, the number
of reproductive organs in shoot decreased. In the
experiment, there were variants with seedling plants of
80-90; 100-110 and 120-130 thousand plants/ha and
the norms of Zerox 1-2-3 l/t; 1-2-3 l/ha + PAV 0,15 l/ha.
Among all the variants, the highest number of shoots
formation and development was observed in variants
4-7-10-13 where the seedling number was left at 80-90
thousand plants/ha. It was found that there were 3,0-
2,6-11,4-4,1 units more than control 1 variant,
respectively.
It was found that the number of matured in variants 5-
8-11-14, with a seedling number of 100-110 thousand
plants/ha, was 0,9-0,4-4,7-5,5 more bushes compared
to the second variant.
Also, in variants 6-9-12-15, with a seedling number of
120-130 thousand plants/ha, the number of plants per
hectare was 2,2-1,2-7,3-8,5 more plants compared to
the third variant. When analyzing data on yield
indicators for 2021-2022, control, Fitovak and Zeroks
were used in the Bukhara-10 cotton variety with a
American Journal Of Agriculture And Horticulture Innovations
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American Journal Of Agriculture And Horticulture Innovations (ISSN: 2771-2559)
seedling thickness of 80-90 thousand plants/ha.
Table -2
The effect of using Zeroks at different standard periods on the yield of cotton grown at different
seedling thicknesses (2021-2023)
As a result, the average three year productivity
indicators in options 1-4-7-10-13 are: 38,1-41,4-39,8-
46,0-45,4 q/ha; in options 2-5-8-11-14 with a seedling
number of 100-110 thousand plant/ha proportionally:
36,0-39,6-38,8-43,6-43,0 q/ha; in variants 3-6-9-12-15,
where the a seedling number was 120-130 thousand
plants/ha, it was 35,1-38,3-37,4-42,2-42,0 q/ha,
respectively.
According to the data of 2023, when the Bukhara-10
cotton variety was maintained at different seedling
thicknesses and Zeroks was applied at different rates
and periods (1-2-3 l/t; 1-2-3 l/ha+PAV 0,15 l/ha), it was
observed that there was a significant difference in the
cotton yield between the options.
In the last phase of the cotton vegetation, the cotton
harvest leaves a seedling number of 80-90 thousand
plant/ha, control, standard, Zeroks 1-2-3 l/t; 1-2-3
l/ha+PAV 0,15 l/ha used in the 1-4-7-10-13 variants,
respectively: 40,8-46,3-44,1-52,5-50,5 q/ha; in variants
2-5-8-11-14, where the seedling number is 100-110
thousand plant/ha, respectively: 36,5-44,2-42,5-50,8-
49,5 q/ha; The seedling number was 35,9-40,4-40,1-
49,7-47,6 q/ha in the 3-6-9-12-15 variants, where 120-
130 thousand plants/ha were left.
CONCLUSION
According to the conducted three year studies, the
highest cotton yield was observed in the variant with a
seedling number of 80-90 thousand plants/ha and the
optimal norm of application of Zerox (2 l/t: 2 l/ha + PAV
0,15 l/ha) (Table 2).
The increase in seedling number and the use of the
immunnostimulant Zerox below or above the standard
level during all growth and development periods of the
Bukhara-10 cotton variety led to a significant decrease
in biological and economic cotton yields. As a result, the
most positive indicator was observed in variant 10, with
an average three-year yield of 46.0 q/ha. It was found
that this yield was 7.6 q/ha higher than that of the
№
Variants
A
number
of
different
seedlings
thousan
d
plants/h
a
2021
2022
2023
An
average
of three
years,
total
harvest
q/ha
Produc
tivity
differenc
e,
q/ha, ±
total
harves
t,
q/ha
total
harves
t,
q/ha
total
harves
t,
q/ha
1
Control
(water)
80-90
38,8
34,6
40,8
38,1
0,0
2
100-110
37,5
33,9
36,5
36,0
0,0
3
120-130
35,7
33,7
35,9
35,1
0,0
4
Fitovak
200 ml/t;
ml/hа
80-90
40,5
37,5
46,3
41,4
+3,3
5
100-110
39,7
34,9
44,2
39,6
+3,6
6
120-130
39,0
35,6
40,4
38,3
+3,2
7
Zerox 1
l/t, l/hа
80-90
40,0
35,4
44,1
39,8
+1,7
8
100-110
39,0
35,0
42,5
38,8
+2,8
9
120-130
38,5
33,5
40,1
37,4
+2,3
10
Zerox 2 l/t,
l/hа
80-90
45,5
40,0
52,5
46,0
+7,9
11
100-110
42,0
38,0
50,8
43,6
+7,6
12
120-130
40,5
36,5
49,7
42,2
+7,1
13
Zerox 3 l/t,
l/hа
80-90
45,2
40,4
50,5
45,4
+7,3
14
100-110
42,3
37,3
49,5
43,0
+7,0
15
120-130
42,0
36,5
47,6
42,0
+6,9
НСР
05
1,69
s/hа
1,21
s/hа
1,26
ц/hа
1,28 ц/hа
P%
4,2%
3,4%
2,8%
3,2 %
American Journal Of Agriculture And Horticulture Innovations
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American Journal Of Agriculture And Horticulture Innovations (ISSN: 2771-2559)
control variant with the same number of seedlings.
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