Volume 04 Issue 01-2024
24
American Journal Of Agriculture And Horticulture Innovations
(ISSN
–
2771-2559)
VOLUME
04
ISSUE
01
Pages:
24-28
SJIF
I
MPACT
FACTOR
(2021:
5.
705
)
(2022:
5.
705
)
(2023:
7.
471
)
OCLC
–
1290679216
Publisher:
Oscar Publishing Services
Servi
ABSTRACT
This study delves into the potential antidiabetic effects of the synergistic combination of soy and yogurt in
Streptozotocin-Nicotinamide induced rats. Through an in-depth analysis, we investigate the biochemical and
physiological impacts of this combination on diabetic markers. The study aims to contribute valuable insights into the
development of alternative approaches for managing diabetes using natural food compounds.
KEYWORDS
Soy, yogurt, antidiabetic, Streptozotocin-Nicotinamide, rats, synergistic effects, biochemical markers, physiological
impact, diabetes management, natural compounds.
INTRODUCTION
Diabetes mellitus, a chronic metabolic disorder
characterized by hyperglycemia, poses a significant
global health challenge. The quest for innovative and
natural approaches to managing diabetes has led to
increased interest in the potential health benefits of
dietary compounds. Among the various dietary
Research Article
SOY-YOGURT SYNERGY: A COMPREHENSIVE EXPLORATION OF ITS
ANTIDIABETIC
POTENTIAL
IN
STREPTOZOTOCIN-NICOTINAMIDE
INDUCED RATS - AN IN-DEPTH ANALYSIS
Submission Date:
December 31, 2023,
Accepted Date:
January 05, 2024,
Published Date:
January 10, 2024
Crossref doi:
https://doi.org/10.37547/ajahi/Volume04Issue01-05
Ismed Kwanariesta
Department of Food Science and Technology, Faculty of Agriculture, Universitas Sumatera Utara, Indonesia
Herla Suhaidi
Department of Food Science and Technology, Faculty of Agriculture, Universitas Sumatera Utara, Indonesia
Journal
Website:
https://theusajournals.
com/index.php/ajahi
Copyright:
Original
content from this work
may be used under the
terms of the creative
commons
attributes
4.0 licence.
Volume 04 Issue 01-2024
25
American Journal Of Agriculture And Horticulture Innovations
(ISSN
–
2771-2559)
VOLUME
04
ISSUE
01
Pages:
24-28
SJIF
I
MPACT
FACTOR
(2021:
5.
705
)
(2022:
5.
705
)
(2023:
7.
471
)
OCLC
–
1290679216
Publisher:
Oscar Publishing Services
Servi
components, soy and yogurt have gained attention for
their individual antidiabetic properties. This study aims
to unravel the synergistic potential of the combination
of soy and yogurt in mitigating diabetes, specifically in
the context of Streptozotocin-Nicotinamide induced
rats.
Soy and yogurt, individually recognized for their
bioactive components and nutritional profile, have
demonstrated promising effects in improving insulin
sensitivity, glucose metabolism, and lipid regulation.
However, their combined impact remains relatively
unexplored, particularly in the context of diabetes
management. Streptozotocin-Nicotinamide induction
in rats serves as a well-established model for studying
type 2 diabetes, offering insights into the efficacy of
potential antidiabetic interventions.
In this comprehensive exploration, we delve into the
intricate biochemical and physiological mechanisms
underlying the antidiabetic potential of the soy-yogurt
synergy. By conducting an in-depth analysis, we aim to
elucidate the combined effects of soy and yogurt on
diabetic markers, providing a foundation for
understanding their collective impact on glucose
homeostasis and insulin regulation.
The findings of this study hold the promise of
contributing valuable insights into alternative and
holistic approaches for diabetes management.
Additionally, the exploration of synergistic effects
between soy and yogurt may pave the way for the
development of functional foods or dietary strategies
aimed at preventing or alleviating the complications
associated with diabetes. As we embark on this journey
of investigation, the integration of soy and yogurt as a
potential antidiabetic intervention represents a novel
and promising avenue for promoting health and well-
being in individuals grappling with diabetes.
METHOD
The experimental protocol adhered to ethical
guidelines and received approval from the Institutional
Animal Ethics Committee (IAEC). Thirty male Wistar
rats, weighing between 180-220 grams, were randomly
divided into five groups: Normal Control, Diabetic
Control, Soy-Yogurt Group, Soy Group, and Yogurt
Group. The diabetes induction was achieved by a single
intraperitoneal injection of Streptozotocin (STZ) at a
dose of 65 mg/kg div weight, 15 minutes after the
administration of Nicotinamide (NA) at a dose of 110
mg/kg div weight.
Following the confirmation of diabetes induction
through periodic blood glucose measurements, the
intervention phase commenced. The Soy Group
received a soy-based diet, the Yogurt Group received a
yogurt-supplemented diet, and the Soy-Yogurt Group
received a combination of both soy and yogurt in their
diet. The Normal Control and Diabetic Control groups
were fed a standard diet without any soy or yogurt
supplementation.
The dietary intervention spanned eight weeks, during
which the rats were closely monitored for changes in
div weight, food intake, and water consumption.
Blood samples were collected at regular intervals to
assess fasting blood glucose levels, serum insulin, and
lipid profile. Intraperitoneal glucose tolerance tests
(IPGTT) were conducted to evaluate glucose handling
and insulin sensitivity.
Histopathological examinations of pancreatic tissues
were carried out to assess the structural changes and
islet cell morphology. In addition, key markers related
to oxidative stress, inflammation, and insulin signaling
pathways were analyzed using molecular techniques
such as RT-PCR and Western blotting.
Volume 04 Issue 01-2024
26
American Journal Of Agriculture And Horticulture Innovations
(ISSN
–
2771-2559)
VOLUME
04
ISSUE
01
Pages:
24-28
SJIF
I
MPACT
FACTOR
(2021:
5.
705
)
(2022:
5.
705
)
(2023:
7.
471
)
OCLC
–
1290679216
Publisher:
Oscar Publishing Services
Servi
Statistical analyses were performed using appropriate
tests, and results were expressed as mean ± standard
deviation. A p-value less than 0.05 was considered
statistically
significant.
The
comprehensive
methodology employed in this study aims to provide a
rigorous and systematic exploration of the antidiabetic
potential of the soy-yogurt synergy in Streptozotocin-
Nicotinamide induced rats.
Animal Selection and Grouping:
Thirty male Wistar rats were selected for the study,
meeting specific weight criteria. Ethical approval was
obtained from the Institutional Animal Ethics
Committee (IAEC). The rats were randomly divided into
five groups: Normal Control, Diabetic Control, Soy-
Yogurt Group, Soy Group, and Yogurt Group.
Diabetes Induction:
Diabetes was induced in the rats using a well-
established
Streptozotocin-Nicotinamide
model.
Streptozotocin
(STZ)
was
administered
intraperitoneally at a dose of 65 mg/kg div weight, 15
minutes after Nicotinamide (NA) administration at a
dose of 110 mg/kg div weight.
Dietary Intervention:
After confirming diabetes induction through blood
glucose measurements, the rats entered an eight-week
dietary intervention phase. The Soy Group received a
soy-based diet, the Yogurt Group received a yogurt-
supplemented diet, and the Soy-Yogurt Group received
a combination of both soy and yogurt in their diet. The
Normal Control and Diabetic Control groups were
given a standard diet without soy or yogurt
supplementation.
Monitoring and Data Collection:
Throughout the intervention, close monitoring
included assessments of div weight, food intake, and
water consumption. Blood samples were collected at
regular intervals to measure fasting blood glucose
levels, serum insulin, and lipid profile. Intraperitoneal
glucose tolerance tests (IPGTT) were conducted to
assess glucose handling and insulin sensitivity.
Histopathological Examination:
At the end of the intervention period, pancreatic
tissues
were
collected
for
histopathological
examination. Changes in islet cell morphology and
overall pancreatic structure were assessed to
understand the impact of the dietary interventions.
Molecular Analyses:
Molecular techniques, including RT-PCR and Western
blotting, were employed to analyze key markers
associated with oxidative stress, inflammation, and
insulin signaling pathways. This allowed for a detailed
exploration of the biochemical and molecular changes
occurring in response to the soy-yogurt synergy.
Statistical Analysis:
Statistical analyses were conducted using appropriate
tests, and results were presented as mean ± standard
deviation. A significance level of p < 0.05 was
considered, ensuring the reliability of the findings.
By meticulously following this multi-faceted process,
our study aims to provide a thorough understanding of
the antidiabetic potential of the soy-yogurt synergy in
Streptozotocin-Nicotinamide induced rats, shedding
light on its biochemical, physiological, and molecular
implications.
RESULTS
Volume 04 Issue 01-2024
27
American Journal Of Agriculture And Horticulture Innovations
(ISSN
–
2771-2559)
VOLUME
04
ISSUE
01
Pages:
24-28
SJIF
I
MPACT
FACTOR
(2021:
5.
705
)
(2022:
5.
705
)
(2023:
7.
471
)
OCLC
–
1290679216
Publisher:
Oscar Publishing Services
Servi
The comprehensive exploration of the antidiabetic
potential of the soy-yogurt synergy in Streptozotocin-
Nicotinamide induced rats yielded noteworthy
findings. The rats in the Soy-Yogurt Group exhibited a
significant reduction in fasting blood glucose levels
compared to the Diabetic Control group. Serum insulin
levels were elevated in the Soy-Yogurt Group,
indicating an improvement in insulin sensitivity.
Additionally, lipid profiles demonstrated favorable
changes, with a decrease in triglyceride levels and an
increase in high-density lipoprotein (HDL) cholesterol.
Histopathological examination revealed preserved
islet cell morphology in the pancreatic tissues of the
Soy-Yogurt Group, contrasting with the observed
structural alterations in the Diabetic Control group.
Molecular analyses demonstrated downregulation of
inflammatory markers and improved expression of
insulin signaling pathway components in the Soy-
Yogurt Group, suggesting a potential mechanism for
the observed antidiabetic effects.
DISCUSSION
The observed improvements in fasting blood glucose,
insulin sensitivity, and lipid profiles in the Soy-Yogurt
Group align with previous studies highlighting the
individual antidiabetic properties of soy and yogurt.
The
synergistic
effects
of
combining
these
components appear to exert a more pronounced
impact on diabetes-related parameters, potentially
through multiple pathways, including enhanced insulin
sensitivity and modulation of inflammatory responses.
The histopathological findings provide morphological
evidence supporting the beneficial effects of the soy-
yogurt synergy on pancreatic islet cells, crucial for
insulin production and regulation. The molecular
analyses further elucidate the mechanisms involved,
indicating a potential role in mitigating inflammation
and enhancing insulin signaling cascades.
Comparisons with the Soy Group, Yogurt Group, and
the Diabetic Control group underscore the unique
contribution of the combined intervention. While soy
and yogurt individually demonstrated positive effects,
their synergy in the Soy-Yogurt Group exhibited a more
comprehensive and significant improvement in various
diabetic markers.
CONCLUSION
In conclusion, this in-depth analysis of the soy-yogurt
synergy's antidiabetic potential in Streptozotocin-
Nicotinamide induced rats provides compelling
evidence of its efficacy. The combination of soy and
yogurt in the diet resulted in a robust improvement in
glucose homeostasis, insulin sensitivity, and pancreatic
morphology. Molecular insights suggest that the
synergistic effects may be attributed to a multifaceted
modulation of inflammatory and insulin signaling
pathways.
These findings hold promise for the development of
functional foods or dietary strategies leveraging the
soy-yogurt synergy to complement conventional
approaches in diabetes management. However,
further research is warranted to elucidate the long-
term effects, optimal dosage, and potential
translational applications in human populations. The
present study contributes valuable insights to the
growing div of knowledge exploring natural, dietary
interventions for diabetes, emphasizing the potential
of synergistic combinations to enhance therapeutic
outcomes.
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Volume 04 Issue 01-2024
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American Journal Of Agriculture And Horticulture Innovations
(ISSN
–
2771-2559)
VOLUME
04
ISSUE
01
Pages:
24-28
SJIF
I
MPACT
FACTOR
(2021:
5.
705
)
(2022:
5.
705
)
(2023:
7.
471
)
OCLC
–
1290679216
Publisher:
Oscar Publishing Services
Servi
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