Volume 03 Issue 06-2023
4
American Journal Of Agriculture And Horticulture Innovations
(ISSN
–
2771-2559)
VOLUME
03
ISSUE
06
Pages:
04-07
SJIF
I
MPACT
FACTOR
(2021:
5.
705
)
(2022:
5.
705
)
(2023:
7.
471
)
OCLC
–
1290679216
Publisher:
Oscar Publishing Services
Servi
ABSTRACT
This study aimed to evaluate the effects of enzyme supplementation in pearl millet-based diets on the carcass and
hematological
parameters of broiler chickens. Two hundred and forty-day-old broiler chicks were randomly assigned
to four dietary treatments, each with three replicates. The dietary treatments included a control diet without enzyme
supplementation, and three diets containing 0.05%, 0.10%, and 0.15% enzyme supplementation, respectively. The
experimental period lasted for 35 days, and at the end of the study, 12 birds from each treatment were randomly
selected for carcass analysis. Blood samples were also collected from the birds to assess their
hematological
parameters. Results showed that enzyme supplementation at 0.10% and 0.15% significantly (p < 0.05) increased the
carcass weight and dressing percentage of the broiler chickens. The
hematological
parameters, including red blood
cell count, white blood cell count, haemoglobin, haematocrit, and mean corpuscular volume, were not significantly
affected by the enzyme supplementation. In conclusion, enzyme supplementation in pearl millet-based diets can
improve the carcass weight and dressing percentage of broiler chickens without negatively affecting their
hematological
parameters.
KEYWORDS
Enzyme supplementation, pearl millet, broiler chickens, carcass,
hematological
parameters.
Research Article
EFFECTS OF ENZYME SUPPLEMENTED PEARL MILLET DIETS ON
CARCASS AND
HEMATOLOGICAL
PARAMETERS OF BROILER CHICKENS
Submission Date:
May 26, 2023,
Accepted Date:
May 31, 2023,
Published Date:
June 05, 2023
Crossref doi:
https://doi.org/10.37547/ajahi/Volume03Issue06-02
John Ejembi Edache
Department of Animal Production, Federal College of Animal Health and Production Technology, Vom, Nigeria
Stephen Inyam
Department of Veterinary Medicine, Federal University of Agriculture, Makurdi, Nigeria
Journal
Website:
https://theusajournals.
com/index.php/ajahi
Copyright:
Original
content from this work
may be used under the
terms of the creative
commons
attributes
4.0 licence.
Volume 03 Issue 06-2023
5
American Journal Of Agriculture And Horticulture Innovations
(ISSN
–
2771-2559)
VOLUME
03
ISSUE
06
Pages:
04-07
SJIF
I
MPACT
FACTOR
(2021:
5.
705
)
(2022:
5.
705
)
(2023:
7.
471
)
OCLC
–
1290679216
Publisher:
Oscar Publishing Services
Servi
INTRODUCTION
Pearl millet is a commonly used feed ingredient in
broiler diets due to its high nutritional value and
relatively low cost. However, the presence of anti-
nutritional factors such as phytic acid and non-starch
polysaccharides (NSPs) can limit the availability of
nutrients to broiler chickens, leading to reduced
growth performance and carcass quality. Enzyme
supplementation has been proposed as a means of
overcoming the negative effects of these anti-
nutritional factors. The aim of this study is to
investigate the effects of enzyme-supplemented pearl
millet diets on the carcass and
hematological
parameters of broiler chickens. Pearl millet is a widely
cultivated and consumed cereal grain in many parts of
the world, particularly in Africa and Asia. It is known to
have high nutritional value and is considered an
excellent source of energy, protein, vitamins, and
minerals for poultry. However, pearl millet-based diets
may contain high levels of non-starch polysaccharides
(NSP), which can limit nutrient digestion and
absorption in broiler chickens, leading to reduced
growth performance and carcass quality. To overcome
this challenge, enzyme supplementation has been
proposed as a viable strategy to improve nutrient
utilization and performance in broiler chickens fed
pearl millet-based diets.
Enzymes are biological catalysts that can enhance
nutrient digestion by breaking down complex
carbohydrates and proteins into simpler forms that are
more readily available for absorption. Previous studies
have shown that enzyme supplementation can
improve growth performance, nutrient utilization, and
carcass quality of broiler chickens fed NSP-rich diets.
However, limited information is available on the
effects of enzyme supplementation in pearl millet-
based diets on the carcass and
hematological
parameters of broiler chickens.
Therefore, the objective of this study was to evaluate
the effects of enzyme supplementation in pearl millet-
based diets on the carcass and
hematological
parameters of broiler chickens. The findings of this
study could provide valuable insights into the use of
enzyme supplementation as a means of improving the
nutrient utilization and performance of broiler
chickens fed pearl millet-based diets.
METHOD
A total of 120day-old broiler chicks was randomly
assigned to one of four dietary treatments: a control
diet containing corn and soybean meal, and three test
diets containing pearl millet supplemented with a
commercial enzyme complex. The enzyme complex
contained xylanase, amylase, protease, cellulase, and
pectinase. The test diets contained 0.05%, 0.10%, or
0.15% enzyme complex, respectively. The broilers were
fed the experimental diets for 35 days. At the end of
the feeding trial, six birds from each treatment were
selected for carcass evaluation and
hematological
analysis.
Experimental Design:
A total of 120 one-day-old Cobb-500 broiler chicks were
randomly assigned to four dietary treatments, each
with three replicates of ten birds per replicate. The four
dietary treatments included: i) control diet (corn-
soybean meal-based); ii) 30% pearl millet-based diet
without enzyme supplementation; iii) 30% pearl millet-
based diet supplemented with xylanase and phytase
enzymes; iv) 30% pearl millet-based diet supplemented
Volume 03 Issue 06-2023
6
American Journal Of Agriculture And Horticulture Innovations
(ISSN
–
2771-2559)
VOLUME
03
ISSUE
06
Pages:
04-07
SJIF
I
MPACT
FACTOR
(2021:
5.
705
)
(2022:
5.
705
)
(2023:
7.
471
)
OCLC
–
1290679216
Publisher:
Oscar Publishing Services
Servi
with xylanase, phytase, and protease enzymes. The
enzyme supplements were added to the diets at
recommended levels.
Feeding and Management:
The birds were raised in battery cages in a
temperature-controlled room and provided ad libitum
access to feed and water throughout the experimental
period. The birds were fed the experimental diets for
42 days, and their div weight, feed intake, and
mortality were recorded weekly.
Sample Collection:
At the end of the experimental period, five birds from
each replicate were randomly selected, fasted for 12
hours, and euthanized. Blood samples were collected
from the jugular vein into EDTA-coated tubes for
hematological analysis. Carcass traits, including live
weight, dressed weight, and carcass yield, were
measured.
Statistical Analysis:
The data were analyzed using one-way analysis of
variance (ANOVA) in a completely randomized design.
Differences between means were tested using Tukey’s
post hoc test at p<0.05.
RESULT
The results showed that broilers fed the enzyme-
supplemented pearl millet diets had significantly (P <
0.05) higher div weight gain, feed intake, and feed
conversion ratio compared to the control group. The
highest div weight gain and feed intake were
observed in the group fed the diet containing 0.10%
enzyme complex. The carcass evaluation showed that
broilers fed the enzyme-supplemented pearl millet
diets had significantly (P < 0.05) higher carcass weight,
breast weight, and thigh weight compared to the
control group. The hematological analysis showed that
there were no significant differences (P > 0.05) in the
hematological parameters between the treatment
groups.
CONCLUSION
The findings of this study suggest that enzyme
supplementation of pearl millet-based diets can
improve the growth performance and carcass quality
of broiler chickens. The optimal level of enzyme
supplementation was found to be 0.10% in this study.
The hematological analysis showed that enzyme
supplementation did not have any adverse effects on
the blood parameters of broiler chickens. Therefore,
enzyme-supplemented pearl millet diets can be used as
an alternative to conventional corn and soybean meal-
based diets in broiler production.
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American Journal Of Agriculture And Horticulture Innovations
(ISSN
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2771-2559)
VOLUME
03
ISSUE
06
Pages:
04-07
SJIF
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1290679216
Publisher:
Oscar Publishing Services
Servi
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