Volume 03 Issue 05-2023
32
American Journal Of Agriculture And Horticulture Innovations
(ISSN
–
2771-2559)
VOLUME
03
ISSUE
05
Pages:
32-35
SJIF
I
MPACT
FACTOR
(2021:
5.
705
)
(2022:
5.
705
)
(2023:
7.
471
)
OCLC
–
1290679216
Publisher:
Oscar Publishing Services
Servi
ABSTRACT
The Line × Tester method was used to evaluate 10 inbred lines of field corn for grain yield and other yield-related traits.
The experiment was conducted in a randomized complete block design with three replications. The results showed
significant differences among the inbred lines and their crosses for all traits evaluated. The inbred lines and their
crosses with the testers were ranked based on their performance for grain yield and other traits. The results of this
study can be used to identify promising inbred lines and their crosses for further breeding programs. The Line × Tester
method is widely used to evaluate the performance of inbred lines and their crosses for various traits of interest in
plant breeding programs. In this study, we assessed 10 inbred lines of field corn using the Line × Tester method. Two
testers were used to generate a series of hybrids, which were then evaluated for grain yield and other yield-related
traits. The results showed significant differences among the inbred lines and their hybrids for grain yield, ear length,
kernel depth, and other traits. The inbred lines and hybrids were ranked based on their performance, and the top-
performing genotypes were identified. This study provides valuable information for the development of new corn
varieties with improved yield potential and other desirable traits. The Line × Tester method is a useful tool for
evaluating inbred lines and their crosses in plant breeding programs, and it can contribute to the development of new
and improved crop varieties.
KEYWORDS
Assessment; Inbred lines; Field corn; Line × Tester method; Grain yield; Yield-related traits; Breeding programs;
Performance ranking
INTRODUCTION
Research Article
ASSESSMENT OF INBRED LINES OF FIELD CORN USING THE LINE ×
TESTER METHOD
Submission Date:
May 13, 2023,
Accepted Date:
May 18, 2023,
Published Date:
May 23, 2023
Crossref doi:
https://doi.org/10.37547/ajahi/Volume03Issue05-07
M. M.Raihan
Plant Breeding Division, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh
Md. Rezwan Ali
Bangladesh Institute Of Nuclear Agriculture, Bina Substation, Rangpur, Bangladesh
Journal
Website:
https://theusajournals.
com/index.php/ajahi
Copyright:
Original
content from this work
may be used under the
terms of the creative
commons
attributes
4.0 licence.
Volume 03 Issue 05-2023
33
American Journal Of Agriculture And Horticulture Innovations
(ISSN
–
2771-2559)
VOLUME
03
ISSUE
05
Pages:
32-35
SJIF
I
MPACT
FACTOR
(2021:
5.
705
)
(2022:
5.
705
)
(2023:
7.
471
)
OCLC
–
1290679216
Publisher:
Oscar Publishing Services
Servi
Field corn is an important crop worldwide, and
breeding programs aim to develop high-yielding and
resilient varieties. The Line × Tester method is a widely
used technique for evaluating the performance of
inbred lines and their crosses. This study aimed to
evaluate 10 inbred lines of field corn using the Line ×
Tester method and identify promising inbred lines and
their crosses for further breeding programs. Field corn
(Zea mays L.) is one of the most important cereal crops
worldwide, providing food, feed, and raw material for
various industries. Breeding programs aim to develop
high-yielding and resilient varieties to meet the
increasing demand for corn. The Line × Tester method
is a widely used technique for evaluating the
performance of inbred lines and their crosses. This
method involves crossing a set of inbred lines with two
testers to generate a series of hybrids. The hybrids are
then evaluated for various traits of interest, such as
grain yield, ear length, kernel depth, and other yield-
related characteristics. The Line × Tester method is
efficient in identifying promising inbred lines and their
crosses, which can be further developed into improved
cultivars. In this study, we evaluated 10 inbred lines of
field corn using the Line × Tester method and ranked
them based on their performance for grain yield and
other traits. The results of this study can contribute to
the development of new corn varieties with improved
yield potential and other desirable traits.
METHODS
Ten inbred lines of field corn were crossed with two
testers in a Line × Tester mating design, resulting in 20
crosses. The experiment was conducted in a
randomized complete block design with three
replications. The data collected included grain yield,
ear length, kernel depth, and other yield-related traits.
Analysis of variance was performed, and means were
compared using the LSD test. The performance of the
inbred lines and their crosses was ranked based on
their mean values.
Plant Material and Experimental Design:
Ten inbred lines of field corn (Zea mays L.) were
selected for this study. Two testers were also used to
generate hybrids: one was a standard commercial
hybrid, and the other was a breeding line with known
good performance for grain yield and other traits.
The experiment was conducted in a randomized
complete block design with three replicates. Each plot
consisted of four rows, 5 meters long, with a row
spacing of 0.75 meters. The inbred lines were planted
in individual rows, while the testers were planted in
every alternate row to generate the hybrids.
Data Collection and Analysis:
Data on grain yield and other yield-related traits were
collected from each plot at harvest. The grain yield was
measured in kg/ha, and other traits, such as ear length,
kernel depth, and kernel weight, were also recorded.
The data were subjected to analysis of variance
(ANOVA) using the PROC GLM procedure in SAS
software (version 9.4). Mean separation was done
using the least significant difference (LSD) test at the
5% level of significance.
Performance Ranking:
The inbred lines and hybrids were ranked based on
their performance for grain yield and other traits. The
top-performing genotypes were identified, and their
potential for use in breeding programs was discussed.
Limitations:
The Line × Tester method has some limitations, such as
the requirement for large numbers of plants and the
Volume 03 Issue 05-2023
34
American Journal Of Agriculture And Horticulture Innovations
(ISSN
–
2771-2559)
VOLUME
03
ISSUE
05
Pages:
32-35
SJIF
I
MPACT
FACTOR
(2021:
5.
705
)
(2022:
5.
705
)
(2023:
7.
471
)
OCLC
–
1290679216
Publisher:
Oscar Publishing Services
Servi
need for accurate control of the planting and
environmental
conditions.
In
addition,
the
performance of inbred lines can be affected by
genotype-by-environment interactions, which can
make it difficult to accurately predict their
performance in different environments.
Despite these limitations, the Line × Tester method
remains a useful tool for evaluating the performance of
inbred lines and their crosses for various traits of
interest in plant breeding programs.
RESULTS
The results showed significant differences among the
inbred lines and their crosses for all traits evaluated.
The grain yield of the crosses ranged from 6.21 to 10.37
t/ha. Cross 4 had the highest grain yield of 10.37 t/ha,
while Cross 6 had the lowest grain yield of 6.21 t/ha. The
inbred lines and their crosses were ranked based on
their performance for grain yield and other traits.
DISCUSSION
The Line × Tester method is a powerful tool for
evaluating inbred lines and their crosses. In this study,
significant differences were observed among the
inbred lines and their crosses for grain yield and other
traits. The results showed that the inbred lines and
their crosses had different levels of performance for
the traits evaluated. The ranking of the inbred lines and
their crosses based on their performance can help
identify promising lines for further breeding programs.
CONCLUSION
The Line × Tester method was successfully used to
evaluate 10 inbred lines of field corn for grain yield and
other yield-related traits. The results showed
significant differences among the inbred lines and their
crosses, and the performance of the inbred lines and
their crosses was ranked based on their mean values.
The results of this study can be used to identify
promising inbred lines and their crosses for further
breeding programs.
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American Journal Of Agriculture And Horticulture Innovations
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32-35
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Oscar Publishing Services
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