ASSESSMENT OF THE ANTIBACTERIAL AND ANTI-FUNGAL ACTIVITY OF THE BIOLOGICALLY ACTIVE SUBSTANCE PREPARED FROM LOCAL PLANT EXTRACTS

Volume 03 Issue 04-2023

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ABSTRACT

In this research work presented, the results obtained in the study of the general pharmacological properties of plant
damalamari with anti-anemia activity, in particular, the effect on the an analysis of the literature on antimicrobial and
pathogenic fungal activity was carried out. All the studies carried out were carried out in different animals, which
serves to increase the reliability level and efficiency values of these experiments. Thus, a fresh decoction, developed
on the basis of local medicinal plants, is less poisonous, it is from local tickling, cumulative and allergic effects. At the
same time, the possibilities of applying the studied extract damalam against microbes and pathogenic fungi mainly in
solution are being studied. The overall safety or non-toxicity of each biologically active substance is important for this
reason, in this article we did not dwell in detail on its main activity.

Research Article

ASSESSMENT OF THE ANTIBACTERIAL AND ANTI-FUNGAL ACTIVITY OF
THE BIOLOGICALLY ACTIVE SUBSTANCE PREPARED FROM LOCAL PLANT
EXTRACTS

Submission Date:

April 20, 2023,

Accepted Date:

April 25, 2023,

Published Date:

April 30, 2023

Crossref doi:

https://doi.org/10.37547/ajahi/Volume03Issue04-06

Bakhramova N.N.

Tashkent Scientific Research Institute for vaccines and serums, Uzbekistan

Abidova R.M.

Tashkent Scientific Research Institute for vaccines and serums, Uzbekistan

Ergashov O.I.

Tashkent Scientific Research Institute for vaccines and serums, Uzbekistan

Boymurodov B.T.

Tashkent Scientific Research Institute for vaccines and serums, Uzbekistan

Qalandarova F.S.

Tashkent Scientific Research Institute for vaccines and serums, Uzbekistan

Journal

Website:

https://theusajournals.
com/index.php/ajahi

Copyright:

Original

content from this work
may be used under the
terms of the creative
commons

attributes

4.0 licence.

Volume 03 Issue 04-2023

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American Journal Of Agriculture And Horticulture Innovations
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SJIF

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(2021:

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5.

705

)

(2023:

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KEYWORDS

General pharmacology plant extract, pathogenic fungal activity, skin resorption, allergic, tickling and cumulative
properties.

INTRODUCTION

As you know, anemia is the main health problem, and
this disease affects people of all ages, especially
pregnant women and children. This cause anemia is
one of the most common non-infectious diseases of
expectant and lactating mothers to date, as well as in
almost the entire segment of newborns. Around the
world, various types of Genesis anemia are being
suffered by residents in the greater scale area and
regions, and this is widely seen as a serious health
problem in nursing mothers. Ensuring the iron needs of
residents of different young sexes is an important task
that does not lose relevance. However, commonly
used sources of synthetic iron cause complications,
and modern prevention of iron deficiency anemia and
pharmacotherapy of this pathology is primarily an
urgent problem in the search and implementation of
natural-structured substances [1-5]. According to
statistics obtained on the basis of epidemiological
studies conducted, about 42% of pregnant women
worldwide and more than 47% of preschool children
suffer from anemia. All types of anemia have serious
adverse effects on human health and development,
including

neonatal

and

perinatal

mortality,

underweight, premature birth, and delayed infant
development. Clinically, anemias are characterized by a
variety of symptoms-notably pallor, fatigue, dizziness,
difficulty breathing, shortness of breath, and
weakness-and if not treated effectively, anemia
contributes to cognitive decline, weakened immunity,
and increased mortality [6-12]. A large-scale scientific
research has been carried out in our country for many

years to study the pharmaco-toxicological properties
of extracts of plants or their biologically active
substances. In particular, studies on the treatment of
oncological diseases of plant alkaloids [14-18], the
search for hepatoprotector agents based on plant
flavonoids [19-23] at the same time, a number of
advances have been made in the study of
neuropsychopharmacological properties [24,25]. In
this regard, as a direct continuation of the scientific
research work carried out, the activity of plant extracts
against anemia in research conditions with the aim of
preventing and treating anemia of different etiologies
and at different levels was studied [26]. Detection of
organ and system hoardiness of extracts with anti-
anemia activity was found to study non-occurrence of
side effects when chronically administered, as well as
not exhibiting their skin resorption, allergy-inducing,
and irritating properties. This article details the results
of the study on the determination of the general
pharmacological indicators described above.

The purpose of the studies carried out is to:
assessment of general pharmacological indicators of
plant extracts with anti-anemia activity.

Object and methods of research. As an object of
research, a tincture of medicinal plants widely used in
folk medicine was used: tubules of Hawthorn, Bird
taron, sweet brain root, water pepper and medicinal
chamomile plants, prepared in a 1:1 ratio.

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All studies carried out were carried out on a normal diet
under vivarian conditions in rabbits with a div weight
of 2-2.5 kg,sea urchins with a div weight of 500 - 600
g, laboratory rats with a div weight of 180-200 g, and
mice with a div weight of 18-24 g.

The effect of the studied substance on the central

nervous system was carried out in the “open field”

method, the methods in which the skin resorption,
tickling the mucous membranes and allergic properties
are presented in the manuals and methodological
recommendations [27].

1.

The results obtained in the study of the local

tickling property of the substance studied.

The local tickling reaction of the tincture studied was
studied in mice, rats and rabbits. To do this, a solution
of various concentrations of the studied tincture was
applied in doses of 0.25 - 0.5 ml to the skin of animals
previously cleaned of feathers and instilled in one eye
of rabbits from 2 - 3 drops, and in the other eye-in these
conditions-discosted water was instilled. Control was
recorded for 2 days. During this time, no reactions
associated with visual tickling were recorded on the
skin of animals or in the eyes of rabbits.

2.

The results of the study of the effect of the

studied tincture on the activity of movement and

search of experimental animals in the ‘open field’

method.

In the studies carried out, it was demonstrated that
this tincture did not adversely affect the activity of
movement and search of experimental animals in
relation to the control group in all doses studied. This,
in turn, makes it possible to conclude that this tincture
does not adversely affect the cognitive and motor
activity of experimental animals, as well as impair the
activity that requires attention.

3.

Assessment of the alaergic effect of the

tincture studied

To determine whether the studied tincture has an
allergic effect, drip a solution of flour of various
concentrations into the eyes of guinea pigs from 1 - 2
drops, the effect of which on the mucous membrane
of the eyes was monitored throughout the day. During
the control, what changes in the mucous membrane of
the animal eye in the experiment were not recorded.

In subsequent experiments, the tincture made from
the collection of plants was repeatedly applied to the
skin of guinea pigs, which had previously been cleaned
of wool. Experiments have shown that there are no
changes characteristic of any apparent allergic
reaction to the eye in the repeated drip site of this
damalam: redness, rash, inflammation, skin peeling
and other reactions that have not occurred. The
general condition of the animals did not differ from the
general condition of the animals in the control group.

Then the allergic effect of tincture made from plant
extracts in guinea pigs was studied using bull whey. For
this, the animals in the experiment were sensitized
with bull serum. On the 21st day of sensitization, a large
dose of bull serum was administered to guinea pigs
who had been doused with discarded water or
tincture, and allergic reactions of almost the same
appearance occurred in both groups of animals. In this,
the breathing of animals was aggravated, sneezing
appeared, the feathers snapped, and 3 of the 6 guinea
pigs in both groups died.

So it turns out that the studied damalam does not have
the ability to bring an allergic reaction to the surface or
prevent it. Taking into account the fact that several
systems of the human div work on the occurrence of
an allergic reaction, it is necessary to consider the
results obtained in laboratory animals as relatively

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relative results when assessing whether the newly
studied collection has an allergic effect.

4.

Study of the cumulative effect of the herbal

collection tincture under study.

Cumulative effect of herbal decoction tincture under
study 18 weight 140-170 g.li studied in labaratory rats.
herbal collection tincture was administered orally on
the first five days of the experiment in the amount of 5
ml/kg. The next five days, however, the drug was
administered with a 2-fold dose increase (10 ml/kg).
Even in the 3rd and 4th five days of the experiment, the
dose of BFC was administered in a volume that was
increased 2 more times than in the previous 5-day dose,
that is, in doses of 20 ml/kg and 40 ml/kg, respectively.

For a total of 20 days, animals in the experimental
group were administered an antianemic collection
tincture

–

a herbal collection tincture, which was being

studied at a dose of 375 ml/kg.

The observation over the experiments was carried out
during the entire experiment and for another 15 days
after the last administered dose of the drug under
vivarian conditions. The main focus in this was on the
general condition of rats, their desire for food and
response reactions to the various constituents given
from the outside.

The results were similar to those obtained in the
control group of the general condition of animals, their
desire for food, response reactions to external
constituents.

The last observation from the experiment was made
lifeless by animals on the 15th day against the
background of mild narcosis, and their internal vital
organinig morphological cases were considered. The
tests carried out showed that the internal organs of the
animals in the experiment did not have any changes.

So, the plant collection under study does not cause any
negative and side effects even when the herbal
collection tincture is administered to animals in the
experiment with a therapeutic dose of more than 15
times its therapeutic dose for 20 days.

Based on the above results, it can be said that herbal
decoction is free from cumulative effects.

5.

Analysis of antimicrobial and pathogenic

fungal activity of herbal tincture.

In this section, based on the data of the studied and
analyzed literature, preliminary results of the use of
this extract of tincture for pathologies occurring on the
skin and mucous membranes are presented [4,5, 28,
29]. Preliminary analysis of extracts of various parts of
of herbal tincture in various solvents for antimicrobial
activity showed that only the root and leaves showed
antibacterial

activity.

Antifungal

activity

was

demonstrated by the root, fruits and seeds. These
parts of the plant were taken for further study. The
overall safety or non-toxicity of each biologically active
substance is important for this reason, in this article we
did not dwell in detail on its main activity.

Conclusions. Medicinal plant tincture experimental
animals were found to have no tickling effect on the
skin and mucous membranes. Also, in the studies
carried out, it did not show an allergic and cumulative
nature, and it can be concluded that there is also no
negative effect on the cognitive indicators of
experimental animals, while in the same period of
movement and search.

Thus, a fresh decoction, developed on the basis of local
medicinal plants, is less poisonous, it is from local
tickling, cumulative and allergic effects.

Preliminary analysis of extracts of various parts of of
herbal tincture in various solvents for antimicrobial

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Servi

activity showed that only the root and leaves showed
antibacterial

activity.

Antifungal

activity

was

demonstrated by the root, fruits and seeds. These
parts of the plant were taken for further study.

The overall safety or non-toxicity of each biologically
active substance is important for this reason, in this
article we did not dwell in detail on its main activity.

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