Volume 04 Issue 12-2024
14
American Journal Of Biomedical Science & Pharmaceutical Innovation
(ISSN
–
2771-2753)
VOLUME
04
ISSUE
12
P
AGES
:
14-17
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
ABSTRACT
The aim of this study is to analyze the stages of morphological development of the parotid salivary glands of rabbits
during postnatal ontogenesis. The paper considers the key stages of differentiation and formation of these glands, as
well as their morphofunctional features at different stages of postnatal development. Using histological and
morphometric analysis, changes in the structure of the glands, as well as their relationship with the development of
other organs of the digestive system, were studied. The results demonstrate significant changes in the morphology
of the parotid salivary glands of rabbits, which occur at different stages of their development. Particular attention is
paid to age-related differences in the cellular structure and functional activity of the glands, which is important for
further studies of the physiology and pathology of the salivary glands in animals. This study contributes to the
understanding of the biological mechanisms of salivary gland formation in mammals.
Research Article
STAGES OF MORPHOLOGICAL DEVELOPMENT OF THE PAROTID
SALIVARY GLANDS OF RABBITS IN POSTNATAL ONTOGENESIS
Submission Date:
December 01, 2024,
Accepted Date:
December 06, 2024,
Published Date:
December 11, 2024
Crossref doi:
https://doi.org/10.37547/ajbspi/Volume04Issue12-03
Chinniyeva M.I.
Elder Professor, Department of Histology and Medical Biology, TGSI, Uzbekistan
Melibayev Orif Yunusovich
Professor of the Tashkent State Dental Institute, Uzbekistan
Nabiyeva Z.B.
Elder Professor, Department of Histology and Medical Biology, TGSI, Uzbekistan
Rixsiyeva M.A.
Assistant of the Department of Histology and Medical Biology, TGSI, Uzbekistan
Soatova M.S.
Assistant of the Department of Histology and Medical Biology, TGSI, Uzbekistan
Journal
Website:
https://theusajournals.
com/index.php/ajbspi
Copyright:
Original
content from this work
may be used under the
terms of the creative
commons
attributes
4.0 licence.
Volume 04 Issue 12-2024
15
American Journal Of Biomedical Science & Pharmaceutical Innovation
(ISSN
–
2771-2753)
VOLUME
04
ISSUE
12
P
AGES
:
14-17
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
KEYWORDS
Parotid salivary glands, rabbits, postnatal ontogenesis, morphological development, morphogenesis, salivary glands,
differentiation, histology, morphofunctional features, cell differentiation.
INTRODUCTION
The salivary glands play an important role in the div
of mammals, participating in the processes of
digestion, moistening the oral cavity and protection
from pathogenic microorganisms. The parotid salivary
glands, in particular, are one of the largest paired
glands that secrete a secretion containing enzymes,
amylose and other substances necessary for digestion
of food. These glands, like other exocrine organs,
undergo a complex process of morphological and
functional development during ontogenesis, which is
important for understanding not only their normal
function, but also the pathology associated with
disturbances in their development. In rabbits, as one of
the common objects of zoological research, the
parotid salivary glands play an important role in
digestion, and their morphological development in the
postnatal period is of particular interest for studying
the mechanism of formation and differentiation of
glandular tissues. However, despite the importance of
these studies, issues related to the morphogenesis of
the parotid salivary glands in rabbits in postnatal
ontogenesis remain insufficiently covered in the
scientific literature. The present study is aimed at
studying the morphological features of the
development of the parotid salivary glands in rabbits in
the postnatal period. The main attention is paid to the
various stages of their development, as well as the
analysis of morphofunctional changes during growth
and formation. The data obtained can serve as a basis
for further research in both normal physiology and
pathology of the salivary glands, which, in turn, is
important for veterinary medicine and biology.
The purpose of the study
The aim of this study is to analyze the stages of
morphological development of the parotid salivary
glands in rabbits during postnatal ontogenesis, with an
emphasis on the key stages of cell differentiation, the
development
of
gland
structures
and their
morphofunctional changes at different age stages.
METHODS
The study involved the use of rabbit carcasses of
various age groups, from newborns to adults. The
animals were obtained from a laboratory nursery and
were used in accordance with the ethical standards for
scientific research.
Morphological examination of the parotid salivary
glands was performed using histological methods. For
this purpose, tissue samples were selected, fixed in 10%
Volume 04 Issue 12-2024
16
American Journal Of Biomedical Science & Pharmaceutical Innovation
(ISSN
–
2771-2753)
VOLUME
04
ISSUE
12
P
AGES
:
14-17
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
formalin solution and then processed with paraffin.
Sections 5-
7 μm thick were stained with hematoxylin
and eosin to assess the general morphology of the
glands, as well as using special methods (for example,
staining according to the Verhoeff method) to
highlight the structural features of the cells and ducts.
To evaluate the morphometric parameters, a light
microscope imaging technique was used with a digital
camera and appropriate software. Cell sizes, duct wall
thicknesses, cell density in different areas of the glands
were measured, and changes in tissue structure were
analyzed depending on the age of the animals.
In addition, to identify functional changes in the
structure of the salivary glands, immunohistochemical
analysis was performed using antibodies to specific
markers of cells involved in the production of saliva.
RESULTS AND DISCUSSION
Morphological examination of the parotid salivary
glands showed that with age, rabbits experience
significant development of the structure of these
glands. In newborn animals, the glands were less
differentiated, with insufficiently developed ducts and
an insignificant number of secretory cells. While in
adult rabbits, clear differentiation of cells, developed
ducts and pronounced secretory activity are observed.
The use of hematoxylin and eosin made it possible to
isolate mature acinar cells in older individuals,
indicating the development of functional maturity of
the glands. While in newborns, immature cells with less
pronounced
secretory
activity
predominated.
Morphometric analysis showed that the size of the
cells and the thickness of the duct walls increase with
age. In newborn rabbits, the cells of the parotid glands
were significantly smaller in size, and the walls of the
ducts were thinner. While in adult animals, the cell sizes
increased and the duct walls thickened, indicating an
increase in the functional activity of the glands. The cell
density also increased with age, which may be due to
the intensity of saliva secretion and the adaptation of
the
glands
to
a
more
active
function.
Immunohistochemical analysis using antibodies to
markers of cells involved in saliva production (e.g.,
amylose and acid mucoprotein) showed that in adult
rabbits the expression level of these markers was
significantly higher than in neonates. This confirms the
hypothesis that with age, the activity of the salivary
glands and their ability to produce saliva increases.
In newborn individuals, markers of saliva production
were poorly expressed, which may be associated with
insufficient maturity of the salivary glands at this age.
CONCLUSION
The obtained data indicate that the parotid salivary
glands of rabbits undergo significant changes during
the process of growth and development. From
newborn to adult individuals, both morphological and
functional changes occur, which correlate with the age
and level of development of the animal.
The increase in cell size and thickening of the duct walls
is associated with an increase in the functional activity
of the glands, which may be due to the div's need for
Volume 04 Issue 12-2024
17
American Journal Of Biomedical Science & Pharmaceutical Innovation
(ISSN
–
2771-2753)
VOLUME
04
ISSUE
12
P
AGES
:
14-17
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
a larger volume of saliva secretion. Increased
expression of saliva production markers in adult
rabbits confirms that with age, the salivary glands
become more active and specialized.
These results can serve as a basis for further research
aimed at studying changes in the salivary glands in
other animal species or humans, as well as for
developing methods to improve the functioning of the
salivary glands in various diseases.
REFERENCES
1.
Chekarova I.A. Comparative morphology of the
parotid salivary gland female male castrate pigs
/I.A. Chekarova agricultural science bulletin-2007-
№1.
- P. 68-71. // Siberian
2.
Chekarova Irina Aleksandrovna Morphology of the
major salivary glands mammals with different
types of nutrition. Abstract Ulan -Ude - 2011.
3.
Stupin Aleksey Viktorovich Morphology of the
parotid, mandibular, sublingual and zygomatic
salivary glands in domestic dogs and fur-bearing
animals animals kept in cages. Abstract. Omsk
–
2010.
4.
Semenova Marina Anatolyevna. Morphometric
features postnatal development of the parotid
salivary gland of rats under conditions nutrition
with dispersed food. Abstract. Orenburg -2011.
5.
Kolipova Yulia Aleksandrovna. Comparative
morphology salivatingly glandular apparatus of
domestic and wild animals (domestic pig, wild wild
boar, brown bear). Abstract. Saransk-2018.
6.
Frolov Evgeny Vyacheslavovich Morphology of the
parotid salivary gland sika deer. Current issues in
veterinary biology No. 2 (14), 2012
7.
Goncharov A.G. Correlation and comparative
analysis of some structural and functional units of
the major salivary glands in various representatives
of the class of mammals. Biological sciences. 2014.
8.
Histology, cytology and embryology / [Afanasyev
Yu. I., Yurina N. A., [Kotovsky E. F. et al. ]; edited by
Yu. I. Afanasyev, N. A. Yurina.
–
[5th ed., [ revised
and enlarged].
–
M.: Medicine.
–
2001.
–
P. 529-538,
597-616.
9.
Histology of the oral cavity organs - S. L. Kuznetsov,
V. I. Torbek , V. G. Derevyanko. - 2012. - 136 p.
10.
Ivanova V.V., Milto I.V., Sukhodolo I.V., Dzyuman
A.N., Modeling hypertrophy of the major salivary
glands in immature rats: morphometric and
histochemical characteristics of epithelial cells.
Bulletin of Siberian Medicine. 2017; 16 (3): 61
–
69.
