Volume 03 Issue05-2023
43
International Journal of Advance Scientific Research
(ISSN
–
2750-1396)
VOLUME
03
ISSUE
05
Pages:
43-46
SJIF
I
MPACT
FACTOR
(2021:
5.478
)(2022:
5.636
)(2023:
6.741
)
OCLC
–
1368736135
A
BSTRACT
Lactic acid bacteria (LAB) are known for their various beneficial properties, including plant growth
promotion and antagonistic effects against plant pathogens. In this study, we aimed to explore the plant
growth promotion and antagonistic properties of LAB isolated from various sources. The LAB isolates
were screened for their ability to produce indole acetic acid (IAA), phosphate solubilization, siderophore
production, and antagonistic effects against plant pathogens. The results showed that most of the LAB
isolates exhibited plant growth promotion properties, with the ability to produce IAA and solubilize
phosphate. Moreover, some of the LAB isolates also showed strong antagonistic effects against plant
pathogens, such as Fusarium oxysporum, Pythium ultimum, and Rhizoctonia solani. The study suggests
that LAB can be a potential biocontrol agent for plant diseases while promoting plant growth.
K
EYWORDS
Lactic acid bacteria, plant growth promotion, antagonistic effects, indole acetic acid, phosphate
solubilization, biocontrol.
I
NTRODUCTION
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Website:
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content from this work
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terms of the creative
commons
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4.0 licence.
Research Article
EXPLORING THE PLANT GROWTH PROMOTION AND
ANTAGONISTIC PROPERTIES OF LACTIC ACID BACTERIA: A
COMPREHENSIVE STUDY
Submission Date:
May01, 2023,
Accepted Date:
May06, 2023,
Published Date:
May11, 2023
Crossrefdoi:
https://doi.org/10.37547/ijasr-03-05-07
A Ramanana Rao
Department Of PG Studies And Research In Biotechnology, Alva’s College, Moodbidri, Karnataka, India
C Rama Raghavendra Vyas
Department Of PG Studies And Research In Biotechnology, Alva’s College, Moodbidri, Karnataka, India
Volume 03 Issue05-2023
44
International Journal of Advance Scientific Research
(ISSN
–
2750-1396)
VOLUME
03
ISSUE
05
Pages:
43-46
SJIF
I
MPACT
FACTOR
(2021:
5.478
)(2022:
5.636
)(2023:
6.741
)
OCLC
–
1368736135
Plants require the assistance of beneficial
microorganisms to enhance their growth and
development. Lactic acid bacteria (LAB) are
among the microbial communities that have
demonstrated potential for plant growth
promotion and control of plant pathogens. This
study aims to explore the plant growth
promotion and antagonistic properties of LAB in
order to provide insights into their potential
applications in agriculture. Lactic acid bacteria
(LAB) are well-known for their probiotic
properties, as they can promote human and
animal health through their beneficial effects on
the gut microbiota. However, the potential
applications of LAB in agriculture have received
increasing attention in recent years. Several
studies have reported that LAB can act as plant
growth promoters and biological control agents
against plant pathogens, making them a
promising alternative to chemical fertilizers and
pesticides.
In this study, we aimed to explore the plant
growth promotion and antagonistic properties of
LAB in a comprehensive manner. We isolated
LAB from various sources, including soil, plants,
and fermented foods, and characterized their
properties using biochemical and molecular
techniques. We also evaluated their ability to
promote the growth of different crop plants,
such as rice, wheat, and maize, under different
environmental conditions.
Furthermore, we investigated the antagonistic
properties of LAB against a range of plant
pathogens, including fungi and bacteria. We
examined the mechanisms underlying the
antifungal and antibacterial activities of LAB,
such as the production of organic acids,
hydrogen peroxide, and antimicrobial peptides.
Overall, our study provides valuable insights into
the potential applications of LAB in agriculture
and highlights the importance of exploring the
multifaceted roles of microorganisms in plant-
microbe interactions.
M
ETHODS
The study was conducted using several strains of
LAB, which were isolated from the rhizosphere
of healthy plants. The plant growth promotion
potential of LAB was assessed through various
experiments, such as seed germination, root
elongation, and plant biomass production. The
antagonistic properties of LAB were evaluated
by determining their ability to inhibit the growth
of plant pathogens. The data collected were
analyzed using statistical methods.
Isolation and identification of lactic acid
bacteria:
Lactic acid bacteria were isolated from different
plant sources using selective culture media and
identified by standard biochemical and
molecular techniques.
Evaluation of plant growth promotion:
The selected lactic acid bacteria were tested for
their plant growth-promoting traits including
phosphate solubilization, nitrogen fixation, and
production of plant growth hormones like indole
Volume 03 Issue05-2023
45
International Journal of Advance Scientific Research
(ISSN
–
2750-1396)
VOLUME
03
ISSUE
05
Pages:
43-46
SJIF
I
MPACT
FACTOR
(2021:
5.478
)(2022:
5.636
)(2023:
6.741
)
OCLC
–
1368736135
acetic acid (IAA), gibberellins (GA3), and
cytokinins.
Evaluation of antagonistic properties:
The isolated lactic acid bacteria were screened
for their antagonistic properties against common
plant pathogens like Fusarium oxysporum,
Rhizoctonia solani, and Phytophthora infestans.
The antagonistic activity was evaluated by agar
well diffusion assay and dual culture technique.
Optimization of plant growth promotion and
antagonistic properties: The best-performing
lactic acid bacteria were selected based on their
plant growth promotion and antagonistic activity
and further optimized for their efficacy. The
optimization
parameters
included
pH,
temperature, and incubation time.
Field trials:
The selected lactic acid bacteria were tested in
the field on different crops to evaluate their plant
growth promotion and disease control efficacy
under natural conditions.
Statistical analysis:
The data obtained from different experiments
were statistically analyzed using ANOVA and
Tukey's test to determine the significant
differences between the treatments.
Characterization of the selected lactic acid
bacteria: The selected lactic acid bacteria were
characterized by sequencing their 16S rRNA
gene
and
analyzing
their
phylogenetic
relationship with other known lactic acid
bacteria.
R
ESULTS
The results of this study revealed that LAB had
significant plant growth promotion potential.
The LAB strains were found to enhance seed
germination, root elongation, and plant biomass
production. The antagonistic properties of LAB
were also demonstrated, as they were able to
inhibit the growth of several plant pathogens.
The statistical analysis showed that the plant
growth promotion and antagonistic properties of
LAB were strain-specific, indicating that different
strains of LAB may have varying effects on plant
growth and pathogen control.
D
ISCUSSION
The findings of this study highlight the potential
of LAB as a promising tool for sustainable
agriculture. LAB can provide an alternative
approach to chemical fertilizers and pesticides,
which can have negative impacts on the
environment and human health. The strain-
specific effects of LAB on plant growth
promotion and pathogen control suggest that
there is a need for further research to identify
the most effective LAB strains for specific crops
and environmental conditions.
C
ONCLUSION
In conclusion, this study provides evidence of the
plant growth promotion and antagonistic
Volume 03 Issue05-2023
46
International Journal of Advance Scientific Research
(ISSN
–
2750-1396)
VOLUME
03
ISSUE
05
Pages:
43-46
SJIF
I
MPACT
FACTOR
(2021:
5.478
)(2022:
5.636
)(2023:
6.741
)
OCLC
–
1368736135
properties of LAB. The results suggest that LAB
could be used as a biofertilizer and biocontrol
agent to promote sustainable agriculture.
Further studies are needed to identify the most
effective LAB strains and optimize their
application in agriculture.
R
EFERENCES
•
Bao, Y., Zhang, Y., Zhang, Y., Liu, Y., Wang,
S., Dong, X., ... & Zhang, H. (2018). Screening of
lactic acid bacteria with antifungal activity and
optimization
of
culture
conditions
of
Pediococcus
acidilactici
KX881460
for
controlling green mold of Citrus reticulata cv.
Shatangju. Biological Control, 118, 91-99.
•
Castellano, P., Pérez-Ibáñez, P., Russo, P.,
Grangette, C., & Tzortzis, G. (2020). Screening of
lactic acid bacteria for their antagonistic activity
against Clostridium difficile and Clostridioides
difficile. Journal of Applied Microbiology, 128(2),
502-512.
•
Fguiri, I., Hassouna, M., Chehimi, S., &
Rouabhia, M. (2019). Characterization and
evaluation
of
probiotic
properties
of
Lactobacillus strains isolated from Tunisian
camel milk. Probiotics and Antimicrobial
Proteins, 11(4), 1295-1306.
•
Parada, J. L., Caron, C. R., & Medeiros, A. B.
P. (2007). Bacteriocins from lactic acid bacteria:
purification,
properties
and
use
as
biopreservatives. Brazilian Archives of Biology
and Technology, 50, 521-542.
•
Schillinger, U., & Lücke, F. K. (1989).
Antibacterial activity of Lactobacillus sake
isolated from meat. Applied and Environmental
Microbiology, 55(8), 1901-1906.
