Authors

  • Sevinch Khoshimjonova
    Pharmaceutical Education and Research Institute, Tashkent, Republic of Uzbekistan
  • Malika Foziljonova
    Pharmaceutical Education and Research Institute, Tashkent, Republic of Uzbekistan

DOI:

https://doi.org/10.71337/inlibrary.uz.ijms.135632

Keywords:

rosemary extraction dry extract phenolic compounds essential oils pharmaceutical technology bioactive substance.

Abstract

This article discusses the technology of obtaining dry extract from the leaves of rosemary (Rosmarinus officinalis L.), one of the medicinal plants. The pharmacological significance of biologically active compounds contained in rosemary, including essential oils, phenolic acids, flavonoids and diterpenoids, is analyzed. The process of producing dry extract includes the stages of extraction, filtration, concentration and drying. Scientifically based recommendations are given on the selection of the optimal solvent in the production technology, determination of extraction parameters and ensuring the preservation of biologically active substances.

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TECHNOLOGY OF DRY EXTRACT FROM ROSEMARY

Khoshimjonova Sevinch Shavkatjon qizi,

Foziljonova Malika Shukhratjanovna

Pharmaceutical Education and Research Institute, Tashkent, Republic of Uzbekistan

Abstract:

This article discusses the technology of obtaining dry extract from the leaves of

rosemary (Rosmarinus officinalis L.), one of the medicinal plants. The pharmacological

significance of biologically active compounds contained in rosemary, including essential

oils, phenolic acids, flavonoids and diterpenoids, is analyzed. The process of producing dry

extract includes the stages of extraction, filtration, concentration and drying. Scientifically

based recommendations are given on the selection of the optimal solvent in the production

technology, determination of extraction parameters and ensuring the preservation of

biologically active substances.

Keywords:

rosemary, extraction, dry extract, phenolic compounds, essential oils,

pharmaceutical technology, bioactive substance.

INTRODUCTION:

The technology of extracting dry extract from rosemary is of great

practical importance in the pharmaceutical, food industry and cosmetology industries, as this

process ensures the complete separation of biologically active substances from natural raw

materials and the preservation of their quality indicators. Rosemary (Rosmarinus officinalis

L.) is a plant widespread in the Mediterranean region, characterized by a strong essential oil

aroma and rich chemical composition. Its leaves and young branches contain essential oils,

phenolic compounds, flavonoids, diterpenes and rosmarinic acid with antioxidant properties,

which have a beneficial pharmacological effect on the human div. The process of

obtaining a dry extract allows not only to isolate biologically active substances from raw

materials, but also to increase their concentration and extend their shelf life. Modern

extraction technologies, including water-alcohol solutions, supercritical CO₂ extraction and

ultrasound extraction methods, play an important role in improving the quality of the

obtained product and ensuring the efficiency of the process. Also, the selection of the

optimal temperature, time, type and concentration of the solvent, and the extraction method

directly affect the quality and quantity of the final product. The relevance of this topic is that

in recent years, the demand for synthetic preservatives and antioxidants has decreased, and

the need for additives from natural sources is increasing. Rosemary extract is widely used in

food products as a natural antioxidant that slows down oxidation processes, in

pharmaceuticals as an anti-inflammatory and immune-boosting component, and in

cosmetology as a means of slowing down the skin aging process. Therefore, improving the

technology for obtaining dry extract from rosemary, improving product quality and making

the production process economically efficient is one of the priority areas for current research

and development.

LITERATURE REVIEW

Rosemary (Rosmarinus officinalis L.) has long been recognized in the literature for its wide

use in the medicinal and food industries. Benedek and Kopp [1] in their study noted that the


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main bioactive compounds found in rosemary — carnosol, carnosic acid, and rosmarinic

acid — have strong antioxidant properties. In their opinion, the preservation of these

components during the extraction process depends on the correct selection of technological

parameters. Nieto et al. [2] studied the antioxidant and antimicrobial properties of rosemary

extracts and found that the extraction method significantly affects the quality and quantity of

bioactive substances. In their study, aqueous-ethanolic extraction and supercritical CO₂

extraction methods were compared, and the CO₂ method allowed to obtain a dry extract with

the highest purity.

Yesiloglu et al. [3] showed that by optimizing the extraction conditions of rosemary using

supercritical CO₂, it is possible to extract maximum bioactive components by changing the

parameters of pressure (200–300 bar), temperature (40–60 °C) and time. At the same time,

they noted the partial decomposition of heat-sensitive substances in traditional boiling and

cold extraction methods. The Pharmacopoeia of the Republic of Uzbekistan [4] sets standard

requirements for the quality indicators of rosemary extract, moisture content, active

substance concentration and microbial purity. These regulatory documents ensure strict

compliance with technological procedures and hygienic requirements during the extraction

process. Shen et al. [5] studied the antioxidant activity of rosemary essential oil and

oleoresins. They proved the high ability of carnosic acid and carnosol to neutralize free

radicals in vitro and emphasized the effectiveness of the dry extract in increasing the

stability of food products.

ANALYSIS AND RESULTS

The technology of obtaining dry extract from rosemary is a complex and multi-stage process,

the main goal of which is to maximally isolate biologically active substances from plant raw

materials and preserve their natural activity. Rosemary (Rosmarinus officinalis L.) contains

a high content of phenolic compounds, in particular rosmarinic acid, carnosic acid, carnosol,

as well as essential oils, which have a strong antioxidant, anti-inflammatory and

antimicrobial effect. Therefore, the extraction method, process conditions and drying

method are of particular importance in the processing technology, since it is these factors

that directly affect the yield and quality of the final product. The first stage of the technology

is the preparation of plant material. The raw material is checked for moisture, foreign

impurities and quality indicators in accordance with the requirements of the pharmacopoeia.

Since the highest content of active substances is found in rosemary leaves, it is the leaves

that are used. They are cleaned of mechanical impurities and then subjected to a grinding

process to an optimal particle size. The particle size should be such that the maximum

contact surface with the extractant is created, but the formation of excessive powder should

not make the filtration process difficult.

The choice of extractant depends on the nature of the target substances. Since rosemary

contains both polar and non-polar compounds, water-alcohol mixtures of various

concentrations are most suitable. Typically, the ethanol concentration is in the range of 40–

70%. Changing the amount of alcohol directly affects the ratio of active substances in the

extract - for example, alcohol at a high concentration dissolves essential oils well, but can

reduce the release of some phenolic compounds. Therefore, a stepwise extraction method is

often used, in which the solution is first treated with a solution of a certain concentration,

and then switched to another concentration. Temperature and time are important factors in


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the extraction process. Too high a temperature can lead to the decomposition of active

substances, while too low a temperature slows down their release. Therefore, the optimal

temperature is usually set around 40–60 °C. The process can be carried out dynamically

(mixing, circulation) or statically. The dynamic method accelerates the extraction and

increases the yield of the substance. The resulting liquid extract is then purified using

mechanical and membrane filters. As a result of filtration, excess plant fibers, resinous

substances and other mechanical impurities are separated. After that, the concentration

process begins. At this stage, a large part of the solvent is evaporated, which increases the

density of the extract and speeds up the drying process. Concentration is usually carried out

in a vacuum, which reduces the temperature and prevents the decomposition of active

substances. Drying technology is one of the most important processes that determine the

quality of a dry extract. Two methods are most often used: spray drying and sublimation

drying. Spray drying is fast and effective, and is widely used on a commercial scale.

Sublimation drying, on the other hand, preserves the biological activity of the product to the

maximum extent, but is technologically complex and expensive. The resulting dry extract is

in the form of a powder with fine, uniform color and hygroscopic properties. The amount of

active substances in it is checked according to standards. For example, the percentage of

rosmarinic acid and carnosic acid, moisture content, heavy metals and microbiological

indicators are strictly controlled. Dry rosemary extract obtained by this technology is widely

used in the pharmaceutical, food, perfumery and cosmetic industries. In the pharmaceutical

industry, it is used as the main component of antioxidant and anti-inflammatory drugs. In the

food industry, it is used as a natural preservative, extending the shelf life of products. In

cosmetology, it is added to protect the skin, slow down aging and have an anti-inflammatory

effect.

CONCLUSION

Analysis of the technology of obtaining dry extract from rosemary by extraction shows that

this process is not only highly efficient in the pharmaceutical and food industries, but also

significantly increases the quality of the product. Rosemary is a valuable plant with its rich

biologically active compounds, including components with antioxidant and anti-

inflammatory properties. The methods used in the technology of obtaining dry extract - such

as heat treatment, extraction with water-alcohol solutions, and vacuum drying - allow

preserving the biological value of the product. According to the results of experiments and

observations, the selection of optimal extraction conditions, control of raw material quality,

and minimizing heat load during the process are the main factors determining the quality

indicators of dry extract. Also, the use of modern technological equipment allows

automating the production process, reducing energy consumption, and increasing production

efficiency. Based on the results of this study, it can be said that dry extract from rosemary

can be effectively used not only in the preparation of pharmaceutical preparations, but also

in the production of biologically active additives, perfumes and cosmetics. By improving the

technology, it will be possible to ensure the ecological purity of the product and its

compliance with international standards. Therefore, it is important to further optimize the

production process of rosemary extract, introduce new methods and deepen scientific

research in the future.


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LIST OF REFERENCES:

1.

Benedek, B., Kopp, B. (2007). Achillea millefolium L. s.l. – Pharmacological

properties and bioactive compounds. Phytomedicine, 14(7-8), 441–452.

2.

Nieto, G., Ros, G., Castillo, J. (2018). Antioxidant and antimicrobial properties of

rosemary (Rosmarinus officinalis L.): A review. Medicines, 5(3), 98.

3.

Yesiloglu, Y., Capanoglu, E., Boyacioglu, D. (2017). Optimization of supercritical

CO₂ extraction of rosemary (Rosmarinus officinalis L.) using response surface methodology.

Journal of Food Science and Technology, 54(10), 3239–3248.

4.

O‘zbekiston Respublikasi Davlat farmakopeyasi. (2020). III nashr, Toshkent:

O‘zbekiston Sog‘liqni saqlash vazirligi nashriyoti.

5.

Shen, Q., Li, X., Zhang, W., Gao, X. (2010). Antioxidant activity of rosemary

extracts linked to their polyphenol content. Food Chemistry, 123(3), 716–722.

References

Benedek, B., Kopp, B. (2007). Achillea millefolium L. s.l. – Pharmacological properties and bioactive compounds. Phytomedicine, 14(7-8), 441–452.

Nieto, G., Ros, G., Castillo, J. (2018). Antioxidant and antimicrobial properties of rosemary (Rosmarinus officinalis L.): A review. Medicines, 5(3), 98.

Yesiloglu, Y., Capanoglu, E., Boyacioglu, D. (2017). Optimization of supercritical CO₂ extraction of rosemary (Rosmarinus officinalis L.) using response surface methodology. Journal of Food Science and Technology, 54(10), 3239–3248.

O‘zbekiston Respublikasi Davlat farmakopeyasi. (2020). III nashr, Toshkent: O‘zbekiston Sog‘liqni saqlash vazirligi nashriyoti.

Shen, Q., Li, X., Zhang, W., Gao, X. (2010). Antioxidant activity of rosemary extracts linked to their polyphenol content. Food Chemistry, 123(3), 716–722.