Volume 03 Issue 12-2023
34
International Journal of Medical Sciences And Clinical Research
(ISSN
–
2771-2265)
VOLUME
03
ISSUE
12
P
AGES
:
34-38
SJIF
I
MPACT
FACTOR
(2021:
5.
694
)
(2022:
5.
893
)
(2023:
6.
184
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
ABSTRACT
The thyroid gland of posterity, got from full-grown with chronic intoxication by pesticides vigor and titanium, have
been studied on 1-90 day after birth using electron microscopy methods. It is revealed that intoxication of the maternal
organism vastly slows the rates of postnatal formation of secretory follicles. It is expected that discovered
ultrastructural changes of the thyrocytes are the morphological substratum of the dysfunctions of the thyroid gland.
KEYWORDS
Thyroid of posterity, pesticides, chronic intoxication.
INTRODUCTION
Research Article
THE TOXIC EFFECT PESTICIDES THROUGH MATERNAL ORGANISM
POSTNATAL GENERATION IN THE THYROID GLANDS
Submission Date:
December 07, 2023,
Accepted Date:
December 12, 2023,
Published Date:
December 17, 2023
Crossref doi:
https://doi.org/10.37547/ijmscr/Volume03Issue12-07
Islomova Shokhista Abdikhalilovna
Tashkent medical academy, Uzbekistan
Xusainova Husnobod Jo’rayevna
Tashkent medical academy, Uzbekistan
Olimjonova Go’zal Olimjonovna
Tashkent medical academy, Uzbekistan
Journal
Website:
https://theusajournals.
com/index.php/ijmscr
Copyright:
Original
content from this work
may be used under the
terms of the creative
commons
attributes
4.0 licence.
Volume 03 Issue 12-2023
35
International Journal of Medical Sciences And Clinical Research
(ISSN
–
2771-2265)
VOLUME
03
ISSUE
12
P
AGES
:
34-38
SJIF
I
MPACT
FACTOR
(2021:
5.
694
)
(2022:
5.
893
)
(2023:
6.
184
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
The use of pesticides is typical for modern agricultural
production in all economically developed countries.
because without their production and applications
agriculture carries huge losses. Of the allowed more
than half of the use of pesticides in the republic
belongs to the groups pyrethroid and pyrazole drugs.
Despite the relatively low toxicity of these compounds,
the probability of their negative impact on the nervous,
endocrine and immune systems remains very high. In
this regard, pesticides pose the greatest danger for
pregnant women and their offspring due to the high
sensitivity of the developing nervous, endocrine and
immune systems of the fetus and newborn to toxic
effects. Shown, that the introduction of even small
doses of pesticides into the div of pregnant and
breastfeeding women females adversely affect the
condition the thyroid gland of their offspring [3,4,5].
Exposure to low doses of pesticides can negatively
affect the morbidity of the population in general, and
especially on indicators of health of women and
children [6]. In this regard, it is currently very the
problem of studying more subtle mechanisms of toxic
action is urgent pesticides, search for means and
methods identification of preclinical negative changes
in the div of people or their offspring, occurring
under the influence of small doses of pesticides. The
results of these studies will undoubtedly contribute to
the disclosure of the mechanisms of toxic the action of
pesticides and allow the development of secondary
prevention measures and pathogenetic therapy of
toxic effects. In addition, they can serve the basis for
revising the hygienic regulations of pesticides.
Purpose of work.
Revealing the structural and functional mechanisms of
the toxic effect of pesticides lambda-qi halotrin (LST)
and fipronil (FPN) on the thyroid and thymus glands of
the offspring under the conditions of their influence
through the mother's div.
METHOD
For In the experiments, an insecticide titanium was
used, registered in the republic by the Uzbek-German
LLC “Euro Team”. Titanium active substance is LST.
Another drug registered in our Republic in as an
effective insecticide, is vigor, an active ingredient
which is the FPN. Physicochemical and toxicological
characteristics vigor and titanium are described in
sufficient detail in our publications [1, 2].
Experiments were carried out on whites adult
virgin female rats Wistar rats weighing 150-170 g, and
sexually mature male rats were used only for
fertilization. Then female rats were divided into three
groups of 45 rats in each. The first (experiment 1) group
of rats was injected through the mouth using a probe
LCT at the rate of 8 mg / kg / daily. The second (control)
group received in the same way. The second
(experiment 2) group of rats FPN was introduced in a
similar way from calculation 3.6 mg / kg / daily, which
Volume 03 Issue 12-2023
36
International Journal of Medical Sciences And Clinical Research
(ISSN
–
2771-2265)
VOLUME
03
ISSUE
12
P
AGES
:
34-38
SJIF
I
MPACT
FACTOR
(2021:
5.
694
)
(2022:
5.
893
)
(2023:
6.
184
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
corresponded to 1/100 of the drug LD50. The third
group of rats that received one the same volume of
sterile physiological solution served as a control.
Administration of both pesticides to experimental
groups rats were carried out daily for 75 days until the
end of experiments. On the 31st day of experiments,
females of all groups mated with males for fertilization.
The onset of pregnancy was monitored by the
presence of sperm during vaginal smears. After the
onset during pregnancy, the females are separated
from the males and placed in separate cages for
further research. Offspring from all groups of animals
were euthanized on 7, 14, 21 and 30 days after birth,
under light anesthesia with ether. After sacrifice, the
blood serum was determined concentration of
thyroxine (T4), triiodothyronine (T3) and thyrotropic
hormone (TTH) by enzyme immunoassay using special
kits the company "Human" (Germany) and the
spectrophotometer "Singl" (Germany). Thyroid gland
(Thyroid gland) and thymus gland (thymus, Tm) was
studied using morphometric, immunohistochemical,
and electron microscopic methods. All digital data are
statistically processed using a package of computer of
programs, differences satisfying P <0.05 were
considered significant.
RESULT
Maternal exposure to pesticides significantly reduced
the rate of growth and formation of thyroid gland in
the offspring. There was a significant decrease in the
mean area of thyroid sections compared with the
corresponding age of the control group, which
indicated a decrease in the volume of the organ as a
whole Average the total area of follicles of all classes,
and, accordingly, the number and height of thyrocytes
in them were significantly lower than the control. A
pronounced lag in the rates of neoplasm and follicle
formation was revealed in the experimental groups.
animals. A decrease in the size of mitochondria, as well
as the components of the endoplasmic reticulum and
the
Golgi
complex
of
thyrocytes,
was
found.Morphological the data fully corresponded to
the indicators of hormones, indicating a significant
decrease in concentration T4 and T3 ... The TSH level is
moderate. decreased, indicating a violation
thyrotropic function of the pituitary gland. Along with
with these, certain disorders of postnatal growth and
development of microenvironmental cells Tm of
offspring Starting from the moment of birth, a
tendency towards a decrease in the number of
epithelio-reticular cells (ERC) per unit area
lobules. Moreover, the average number of ERK
decreased more clearly in the cortical the thymus zone.
Electron microscopy in the ERC of experimental
animals revealed hypoplasia of the endoplasmic
reticulum and the Golgi complex, as well as the
heterogeneity of secretory vacuoles, indicating a
violation of the secretory activity of cells All this
testified about slowing down the formation process
Volume 03 Issue 12-2023
37
International Journal of Medical Sciences And Clinical Research
(ISSN
–
2771-2265)
VOLUME
03
ISSUE
12
P
AGES
:
34-38
SJIF
I
MPACT
FACTOR
(2021:
5.
694
)
(2022:
5.
893
)
(2023:
6.
184
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
cellular microenvironment in the thymus and violation
of the secretory function of the ERK, which
contributed to the disruption of regulatory thymus
activity.
CONCLUSIONS
Chronic intoxication of the div mothers with
pesticides leads to a significant slowdown in the rates
of postnatal growth and formation of thyroid
secretory follicles in the offspring. Ultrastructural
changes in the form disorganization of the granular
endoplasmic reticulum and destruction of other
cytoplasmic organelles of thyrocytes represent a
morphological substrate dysfunction of the thyroid
gland arising in the postnatal ontogenesis of the organ
under conditions of chronic intoxication.
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International Journal of Medical Sciences And Clinical Research
(ISSN
–
2771-2265)
VOLUME
03
ISSUE
12
P
AGES
:
34-38
SJIF
I
MPACT
FACTOR
(2021:
5.
694
)
(2022:
5.
893
)
(2023:
6.
184
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
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