Volume 03 Issue 08-2023
52
International Journal of Medical Sciences And Clinical Research
(ISSN
–
2771-2265)
VOLUME
03
ISSUE
08
P
AGES
:
52-57
SJIF
I
MPACT
FACTOR
(2021:
5.
694
)
(2022:
5.
893
)
(2023:
6.
184
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
ABSTRACT
I’m The organism's reactivity to stress, endurance, changing living conditions, extreme situations and other stressful
effects is the main criterion of the organism's adaptation and vitality.
In this study, laboratory rats were divided into groups according to the level of activity, and their various behaviors
were evaluated in the "open field" test. In the test, the rats were released into the central sector of the arena, and the
horizontal and vertical movement activity, the number of looks into the holes, and the number of defecation were
recorded. Behavioral activity of animals was determined during 3-minute observation and the results were compared.
KEYWORDS
"Open space", low active, medium active, high active.
INTRODUCTION
The study of individual typological characteristics of
the response of the central nervous system (CNS) of
humans and animals to the influence of environmental
factors and the mechanisms of disease occurrence is
one of the most urgent problems among medical and
biological sciences today. In recent decades, a special
place in research has been taken by resistance in terms
of the div's resistance to the effects of physical and
emotional stressors, which determine an individual set
of defense mechanisms against stress (Ashmarin, 1999,
2001; Seredenin, 2005). The reactivity of the organism
to stress, resistance to stress, changing living
Research Article
DETERMINATION OF INDIVIDUAL TYPOLOGICAL CHARACTERISTICS OF
THE BEHAVIOR OF RATS USING THE "OPEN FIELD" METHOD
Submission Date:
August 17, 2023,
Accepted Date:
August 22, 2023,
Published Date:
August 27, 2023
Crossref doi:
https://doi.org/10.37547/ijmscr/Volume03Issue08-07
Bafaev Jamshid Turabovich
Tashkent State Institute Of Stomatology, Uzbekistan
Journal
Website:
https://theusajournals.
com/index.php/ijmscr
Copyright:
Original
content from this work
may be used under the
terms of the creative
commons
attributes
4.0 licence.
Volume 03 Issue 08-2023
53
International Journal of Medical Sciences And Clinical Research
(ISSN
–
2771-2265)
VOLUME
03
ISSUE
08
P
AGES
:
52-57
SJIF
I
MPACT
FACTOR
(2021:
5.
694
)
(2022:
5.
893
)
(2023:
6.
184
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
conditions, extreme situations and other stressful
effects is the main criterion of adaptation and vitality
of the organism. At the same time, it has been shown
that the typological characteristics of humans and
animals are closely related to various physiological
indicators [1,2,6,7]. In the literature, there is a
description of the influence of the individual -
typological characteristics of the nervous system,
which have a genetic basis and determine the different
reactions of the organism to various stress factors and
the nature of adaptation. It can be seen that the
typological division is promising for an adequate
assessment of the state of physiological systems in
normal and severe conditions, which is confirmed by
many studies conducted in various fields of physiology
and experimental medicine [3,4,5,6].,7]. The study of
the disease through the approach to individual
typological characteristics of behavior is adaptive
reactions allows for a deeper understanding of the
mechanisms and to identify new differential
approaches to the prevention and treatment of various
diseases, in particular, diabetes and its complications .
The purpose of the study: to evaluate the behavior of
laboratory rats with different behaviors in the "Open
Field" test.
MATERIALS AND METHODS
The experiment was conducted on 56 adult white male
rats weighing 250-300 g, which were kept in standard
vivarium conditions. All experiments were performed
in accordance with the requirements of the European
Convention for the Protection of Vertebrate Animals
Used for Experimental or Other Scientific Purposes
(Strasbourg, 1986). Behavioral actions were studied
according to the methodology of Buresh Ya.I (1991) [9].
The test consists in quantifying the components of the
behavior of an animal placed in a new open field, which
is prevented from exiting by a wall surrounding the
field . Regrouping, feeding, collection and other active
manipulations of the animals were excluded at least 1
h before the test. The experiment was conducted in
complete silence without extraneous sounds. The
"open field" is a well-lit square arena 1 m by 1 m and 45
cm high, the floor of which is marked by square lines.
The rat was released into the central sector of the
arena and the horizontal, vertical movement activity,
the number of looks into the holes, and the number of
defecation were recorded. Behavioral activity of the
animals was determined during a 3-min observation in
voluntary units. According to the results of the "open
field" test, all animals were divided into three groups
using cluster analysis: high active, low active and
medium active. Even in the literature, a highly active
type of behavior is called active search, and a low active
type is called passive-defense [10].
The results of experimental studies were statistically
processed using Excel Microsoft software. Data are
presented as mean (M) and standard error of the mean
Volume 03 Issue 08-2023
54
International Journal of Medical Sciences And Clinical Research
(ISSN
–
2771-2265)
VOLUME
03
ISSUE
08
P
AGES
:
52-57
SJIF
I
MPACT
FACTOR
(2021:
5.
694
)
(2022:
5.
893
)
(2023:
6.
184
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
(m). Student's t test was used to test statistical
hypotheses about differences between study groups.
A difference at a probability level of 95% or more was
considered a statistically significant change (p < 0.05).
Results In order to determine the individual typological
characteristics of the animals' behavior, the analysis of
the parameters of the rats' behavior in the "open field"
(Table 1) showed that the average div weight of all
animals did not differ. The main manifestations of
individual typological characteristics of the behavior of
rats were movement along the surface of the "open
field", calculated by the number of crossed squares.
The horizontal activity of the animals was assessed by
clicking on the peripheral and central squares. The
number of intersections of peripheral squares, that is,
in animals with an active type, this indicator was higher
than in other groups of animals; rats crossed an
average of 28.4 ± 0.9 peripheral squares, while passive
animals had a statistically significant 7.4 times less.
Average value was obtained in moderately active rats
compared to other groups of animals. A significant
change was also found in the value of the intersection
of the central squares, but this change was not as sharp
as the intersection of the peripheral squares, that is,
this indicator in the active type of animals was
statistically significantly 1.86 times more than in the
animals of the passive type.
When comparing the data with the moderately active
group, the number of intersections was 1.4 times
higher. When analyzing the total number of square
crossings, rats in the active group had 5 times more
than passive rats and 2.3 times more than rats in the
medium active group. In passive rats, this indicator was
2.2 times lower than in the group of animals with
moderately active behavior. According to the data, the
animals with high activity moved mainly along the
periphery of the "open field" and rarely went out to its
central part. Animals of the active type made 84.3% of
the total physical activity in the periphery of the "open
field" and 15.7% in the center, and in the group of
animals of the passive type, 57.3% in the periphery and
42.7% in the center. In animals in the moderately active
group, the ratio was 75.2% and 24.6%, respectively.
When comparing the values of the groups, the vertical
activity index did not differ significantly. In all groups
of animals, the maintenance period was very low and
short and did not differ significantly from each other.
Most of the animals in the entire sample left a mean
number of boluses in the arena, which did not differ
significantly between groups, indicating that the
animals had a relatively low level of anxiety, which may
be due to the fact that all animals were well fed before
the start of the experiment.
A group of animals with low-active and medium-active
types of behavioral reactions showed a decrease in
horizontal movement activity in the central and
Volume 03 Issue 08-2023
55
International Journal of Medical Sciences And Clinical Research
(ISSN
–
2771-2265)
VOLUME
03
ISSUE
08
P
AGES
:
52-57
SJIF
I
MPACT
FACTOR
(2021:
5.
694
)
(2022:
5.
893
)
(2023:
6.
184
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
peripheral parts of the "open field". It is known from
the literature that the decrease in movement activity
indicators is an indicator of the manifestation of the
protective indicator that occurs in response to stress
[10]. An increase in this indicator indicates the
occurrence of strong emotional stress of a negative
nature, for example, anxiety, tension, restlessness [10,
11].
Table 1
Indicators of behavioral activity of laboratory rats with different types of behavior in the "open field" test
Groups
Indicators
Body
weight, gr
Number of
intersecting
peripheral
squares
Number of
intersecting
central
squares
Total
number of
squares
cut
Vertical
activity
Chislo
grooming,
sht
The
number of
defecation
Medium active 288.7±1.5
11.2±0.3
3.67±0.15
14.9±0.3
3.18±0.20 4.80±0.22 1.65±0.16
Passive
298.3±7.3
P
2
>0.05
3.83±0.37
P
2
<0.001
2.85±0.13
P
2
<0.01
6.68±0.32
P
2
<0.001
2.73±0.31
P
2
>0.05
4.24±0.21
P
2
>0.05
1.41±0.21
P
2
>0.05
Active
288.8±1.4
P
1
>0.05
P
2
>0.05
28.4±0.9
P
1
< 0.001
P
2
<0.001
5.31±0.24
P
1
< 0.001
P
2
<0.01
33.7±1.0
P
1
<
0.001
P
2
<0.001
2.76±0.24
P
1
>0.05
P
2
>0.05
4.76±0.20
P
1
>0.05
P
2
>0.05
1.92±0.17
P
1
>0.05
P
2
>0.05
Note: P 1 - in relation to the group of animals of the passive type;
P 2
–
indicator for the group of moderately active animals;
Thus, the results obtained from the experiment
showed that according to the characteristics of the
horizontal movement activity of the animals evaluated
using the "open field" test , animals can be
conditionally divided into three groups: animals with
high activity. - active-search type, animals with
moderate activity - intermediate type and animals with
low activity - passive-defense type. According to the
scientific literature, it can be assumed that the
typological characteristics of rats divided into groups
reflect physiological and biochemical differences
based on the assessment of the stress behavior of rats
exposed to an unfamiliar environment in the "open
field" test. Therefore, in experimental animals, since
they belong to different individual typological groups,
Volume 03 Issue 08-2023
56
International Journal of Medical Sciences And Clinical Research
(ISSN
–
2771-2265)
VOLUME
03
ISSUE
08
P
AGES
:
52-57
SJIF
I
MPACT
FACTOR
(2021:
5.
694
)
(2022:
5.
893
)
(2023:
6.
184
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
there may be differences in the course of pathological
conditions and the div's reaction to pathologies.
CONCLUSION
1. Based on the assessment of stress behavior of rats in
the "open field" test, it can be assumed that the
typological characteristics of rats reflect physiological
and biochemical differences.
2. Since experimental animals belong to different
individual typological groups, there may be differences
in the course of pathological conditions and the div's
reaction to pathologies.
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