Volume 03 Issue 07-2023
35
International Journal of Medical Sciences And Clinical Research
(ISSN
–
2771-2265)
VOLUME
03
ISSUE
07
P
AGES
:
35-39
SJIF
I
MPACT
FACTOR
(2021:
5.
694
)
(2022:
5.
893
)
(2023:
6.
184
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
ABSTRACT
Histological and immunohistochemical research methods make it possible to reliably judge the degree of dysplastic
changes in the epidermis in AK and its invasive potential. However, in most cases, the sampling of biopsy material
causes certain difficulties, as it can leave cosmetic defects. Considering that the most common localization of AK is
the skin of the face, it seems relevant to use more widely non-invasive research methods in the diagnosis and
determination of the nature of the growth.
KEYWORDS
Medicine. Methodology, methodology, dermatovenereology, Modern methods.
INTRODUCTION
One of the criteria for the level of dysplasia is the
proliferative activity of cells [Chaichamnan K., 2010;
Nazarian R.M., 2009], which largely determines their
growth rate and malignant potential. The most
informative way to visualize and assess the
proliferative
activity
of
cells
is
an
immunohistochemical study, since markers reveal not
only cells in mitosis itself, but also those that are in the
process of preparing for division and, therefore,
indicate a proliferative potential [Kushlinsky N.E. et al.,
2001; Petrov C.V. et al., 2004]. Numerous studies of
tumors from various tissues indicate a relatively low
proliferative activity of benign tumor cells, while
malignant processes have a high level of proliferation
Research Article
MODERN METHODS OF TREATMENT OF FLAMING NEVUS
Submission Date:
July 10, 2023,
Accepted Date:
July 15, 2023,
Published Date:
July 20, 2023
Crossref doi:
https://doi.org/10.37547/ijmscr/Volume03Issue07-07
U.A. Tashkenbayeva
Head Department, Prof., D.M.S. Department Of Dermatovenereology Tashkent Medical Academy, Uzbekistan
M.R. Mukhamedov
Lecturer, Department Of Dermatovenereology Tashkent Medical Academy, Uzbekistan
Journal
Website:
https://theusajournals.
com/index.php/ijmscr
Copyright:
Original
content from this work
may be used under the
terms of the creative
commons
attributes
4.0 licence.
Volume 03 Issue 07-2023
36
International Journal of Medical Sciences And Clinical Research
(ISSN
–
2771-2265)
VOLUME
03
ISSUE
07
P
AGES
:
35-39
SJIF
I
MPACT
FACTOR
(2021:
5.
694
)
(2022:
5.
893
)
(2023:
6.
184
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
[Chaichamnan K. Et al., 2010; Nazarian R.M. et al.,
2009]. Previously, it was shown that AK has a low
proliferative activity of cells compared to cancer in situ
and squamous cell carcinoma [Bordbar. A.D., 2007;
Talghini S., 2009]. In addition, there is a relationship
between the level of proliferation of keratinocytes and
an increase in the amount of elastic material in the
dermis in AK and cancer in situ [Chang Geun Cho, 1999].
Given the isolated nature of the studies, it seems very
important to continue studying the correlation
between the proliferative potential of cells and the
nature of dermal elastosis, not only in benign and
malignant skin tumors, but also in various types of AK.
This study will also allow evaluating the prospects of
using elastosis ,d,derma as an indirect morphological
marker of aggressive AK growth.
The main results and findings
Currently, due to the advent of sensors with a pulse
generation frequency of 50, 75 and 100 MHz, which
allow differentiating the epidermis and dermis, the
total thickness of which does not exceed 5 mm,
ultrasound is beginning to be actively used in
dermatology [Jasaitiene D., 2011]. The expediency of
using ultrasound in the diagnosis of a number of skin
tumors, assessing the condition of the skin in chronic
dermatoses during therapy has been shown [Bakulev
A.L., 2009; Kurdina M.I., 2009]. Given the lack of data
on the study of AK using ultrasound, it seems relevant
to identify its ultrasonic features for the subsequent
use of high-frequency ultrasound in non-invasive
diagnostics.
PURPOSE OF THE STUDY
To develop additional diagnostic criteria for actinic
keratosis based on the study of immunomorphological
and ultrasound features of the tumor.
Research objectives:
1) To study the features of the clinical course of actinic
keratosis.
2) To study the frequency of histological types of
actinic keratosis and the level of epidermal dysplasia in
each of them.
3) Determine the nature of elastin expression in the
dermis in actinic keratosis.
4) To study the level of expression of the proliferation
marker Cl 67 in the epidermis and determine its
correlation with the nature of the distribution of
antibodies to elastin in the dermis.
5) Determine the ultrasound signs of actinic keratosis
and confirm them with morphological research
methods.
Scientific novelty
1) For the first time, the frequency of histological types
of actinic keratosis and the level of epidermal dysplasia
in each of them were determined;
Volume 03 Issue 07-2023
37
International Journal of Medical Sciences And Clinical Research
(ISSN
–
2771-2265)
VOLUME
03
ISSUE
07
P
AGES
:
35-39
SJIF
I
MPACT
FACTOR
(2021:
5.
694
)
(2022:
5.
893
)
(2023:
6.
184
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
2) For the first time, the variable nature of the
proliferative activity of cells was established in various
histological types of actinic keratosis
3) For the first time, the features of the expression of
antibodies to elastin and variants of its distribution in
the dermis in actinic keratosis have been established.
4) For the first time, based on an immunohistochemical
study, a direct relationship was revealed between the
level of proliferative activity of cells in the epidermis
and the nature of elastin expression in the dermis.
5) For the first time, ultrasonic signs of actinic keratosis
were described and compared with the expression of
elastin in the dermis during an immunohistochemical
study..
Practical value
1) A direct relationship has been established between
the level of proliferative activity of epidermal cells and
the nature of elastin expression in the dermis, which
makes it possible to use the level of elastosis as an
indirect marker of aggressive tumor growth
2) An ultrasonic method for diagnosing actinic
keratosis has been developed and put into practice.
The main ultrasound sign of actinic keratosis is the
presence in various parts of the dermis of a strip-like
hypoechoic zone (narrow, wide or total), which
coincides with the distribution of elastin in the dermis.
3) The width of the hypoechoic zone, detected by a
non-invasive ultrasound method for diagnosing actinic
keratosis, makes it possible to indirectly judge the
proliferative activity of epidermal cells.
An increased incidence of malignant neoplasms of the
skin in the population in regions with excessive
insolation has been observed for many years [1].
Numerous studies show that ultraviolet radiation in
certain spectra plays a leading role in the development
of skin cancer. It is known that the stratum corneum of
the epidermis retains about 90% of the incident light,
and the presence of melanin pigment is a filter for
carcinogenic wavelengths [2]. However, with
excessive UV radiation, the protective properties of
the epidermis are not enough, as a result of which it
penetrates to the deep layers of the dermis, causing
precancerous dermatoses, which can subsequently
lead to malignant neoplasms.
Patients with certain genetic syndromes are also at
increased risk of solar keratosis. These syndromes are
characterized by chromosomal cell damage in
response to the mutagenic action of UV radiation.
These include albinism, xeroderma pigmentosum,
Rothmund-Thompson,
Bloom,
and
Cockayne
syndromes [1]. The most well-known disease is
xeroderma pigmentosum, which is based on a defect
in DNA repair, leading to increased cell sensitivity to UV
rays. The earliest clinical sign of xeroderma pigmentosa
is photodermatitis of open skin areas, which occurs
Volume 03 Issue 07-2023
38
International Journal of Medical Sciences And Clinical Research
(ISSN
–
2771-2265)
VOLUME
03
ISSUE
07
P
AGES
:
35-39
SJIF
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MPACT
FACTOR
(2021:
5.
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)
(2022:
5.
893
)
(2023:
6.
184
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
even with minimal insolation. In particular, cases of
development of solar keratosis in children suffering
from pigment xeroderma are described. [2].
CONCLUSION
The main factors involved in the pathogenesis of AK
are UV rays of the spectra A and B. They cause damage
to the DNA of keratinocytes and the occurrence of
mutations in the p53 gene responsible for suppressing
the uncontrolled growth of genetically defective and,
therefore, potentially tumor cells. Also, UV radiation of
these spectra has a pronounced immunosuppressive
effect, which limits the ability of Langerhans cells to
recognize and destroy atypical, proliferating cells [2].
To date, a number of researchers are trying to prove
the role of human papillomavirus (HPV) in the
pathogenesis of AK. So, according to some authors, in
pathological foci, by quantitative real-time PCR, viral
DNA 5, 8, 15, 20, 24, 36 HRU types are detected.
Moreover, the amount of NRU DNA detected in AK is
significantly higher than in the foci of squamous cell
carcinoma taken as a control group [5]. According to
the results of other studies, there is no direct
relationship between human papillomavirus infection
and the development of AK, however, in combination
with key risk factors
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International Journal of Medical Sciences And Clinical Research
(ISSN
–
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VOLUME
03
ISSUE
07
P
AGES
:
35-39
SJIF
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FACTOR
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OCLC
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1121105677
Publisher:
Oscar Publishing Services
Servi
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