MODELS AND METHODS IN MODERN SCIENCE
International scientific-online conference
133
DIFFERENCES IN IMMUNOHISTOCHEMICAL MARKER EXPRESSION
ACROSS AGE GROUPS IN POST-TRAUMATIC NASAL POLYPOSIS
Yuldashev Kahramonjon Shukurjon ugli
Fergana Public Health Medical Institute., assistant.
Mahkamov Nosirjon Juraevich
Andijan State Medical Institute, DSc, Professor
https://doi.org/10.5281/zenodo.17008023
Introduction
Nasal polyposis (NP) is a chronic inflammatory disease of the sinonasal
mucosa, often associated with structural abnormalities such as post-traumatic
nasal septum deviation. Persistent inflammation following trauma induces
repeated immune activation, leading to polyp formation and remodeling of the
mucosal architecture. The immune response, however, is strongly influenced by
patient age, resulting in distinct patterns of immunohistochemical marker
expression. Understanding these differences is crucial for clarifying disease
pathogenesis and developing age-specific therapeutic approaches.
Objective
To investigate age-related differences in the expression of
immunohistochemical markers (CD3, CD20, CD68, IFN-γ, IL-4, IL-5, IgE, and
TGF-β) in patients with post-traumatic nasal polyposis.
Materials and Methods
1. The study included
40 patients
aged 18–60 years diagnosed with post-
traumatic nasal polyposis.
2. Patients were divided into two age groups:
-
Group I: 18–35 years (20 patients)
-
Group II: 36–60 years (20 patients)
3. Polyp tissue samples were collected during endoscopic surgery.
4. Immunohistochemical staining was performed to assess the expression
of CD3, CD20, CD68, IFN-γ, IL-4, IL-5, IgE, and TGF-β.
5. Quantitative analysis was carried out using morphometric evaluation and
comparative statistics between the two groups.
Results
1. Younger patients (18–35 years):
- Higher infiltration of CD3+ T-lymphocytes and CD20+ B-lymphocytes was
detected.
- Expression of IL-4 and IL-5 was significantly increased, indicating a
dominant Th2 immune response.
MODELS AND METHODS IN MODERN SCIENCE
International scientific-online conference
134
- Local IgE accumulation was frequently observed, accompanied by
eosinophil-rich infiltration.
- Clinically, these patients demonstrated recurrent disease episodes and a
more active inflammatory course.
2. Older patients (36–60 years):
- Predominant infiltration of CD68+ macrophages and increased fibroblast
activity were observed.
- Expression of IFN-γ was elevated, suggesting a dominant Th1 immune
response.
- TGF-β expression was markedly higher, consistent with fibrotic
remodeling processes.
- Clinically, patients demonstrated a more chronic, stable disease course,
with less frequent acute recurrences compared to younger patients.
Conclusion
Post-traumatic nasal polyposis demonstrates distinct age-related
differences in immunohistochemical marker expression:
1. Younger patients exhibit a Th2-skewed immune profile with increased
eosinophilic infiltration and IgE expression, correlating with an active and
recurrent clinical course.
2. Older patients demonstrate a Th1-predominant immune profile with
macrophage infiltration and fibrotic remodeling mediated by TGF-β, correlating
with a more stable but chronic disease progression.
These
findings
underscore
the
importance
of
age-specific
immunopathological evaluation in guiding personalized treatment strategies for
post-traumatic nasal polyposis.
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