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STUDY OF THE STRUCTURAL INFLUENCE ON THE COMPARATIVE
SOLUBILITY OF WATER-SOLUBLE POLYSACCHARIDES ISOLATED
FROM THE FUNGUS INNONOTUS HISPIDUS GROWING IN THE
TERRITORY OF UZBEKISTAN.
Kimsanova G.S.
Tashkent Pharmaceutical Institute
Normahamatov N.S.
Tashkent Pharmaceutical Institute
nodirali@gmail.com
https://doi.org/10.5281/zenodo.13670240
ABCTRACT
: A study was conducted on the influence of monosaccharide
structure on the specific solubility of water-soluble polysaccharides obtained
from Innonotus hispidus basidiomycetes, a fungus belonging to the
Hymenochetaceae family growing on mulberry trees in the Republic of
Uzbekistan. The raw materials were deresinated, purified, and re-extracted with
water. The ratio of solubility in various solvents was determined, with the
highest solubility observed in the ratio of Arabinose and Xylose compounds,
reaching 12.5%. These studies will serve as a basis for further investigation of
the biological activity of this object and its application in medicine and
pharmaceuticals.
Keywords
: Innonotus hispidus, extraction, monosaccharides, solubility,
polysaccharides
These studies will serve as a basis for further study of the biological activity
of this object and its application in medicine and pharmaceuticals.
Water-soluble polysaccharide extraction was carried out in the following
stages:
To extract water-soluble polysaccharides, the defatted raw material was dried to
remove solvent odors, weighed, and extracted three times in a hot water bath
with a reflux condenser (total ratio of raw material to extractor 1:4, 1:3, 1:2).
The total duration of the three extractions was 6 hours. The obtained aqueous
extracts were combined and re-evaporated in a film evaporator at a temperature
ranging from 50°C to 1/5 of the initial volume, then filtered. Water-soluble
polysaccharides were isolated from the obtained concentrate by adding a four-
fold volume of 95% ethyl alcohol and storing at -5°C for 12 hours. After
centrifugation, the precipitate was separated, washed with ethyl alcohol in a
glass filter, and dried at 30°C [1].
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To extract pectin substances from basidiomycetes, after water extraction,
an extract was obtained using a 1.1% hydrochloric acid solution (1:10) at 95°C
(water bath) for 2 hours. The extract was filtered through a nylon cloth,
evaporated, dialyzed in water, and centrifuged (10 min, 6000 rpm). It was then
evaporated again and precipitated with alcohol. The resulting precipitate was
separated by centrifugation (6000 rpm, 10 min) and dried with alcohol. The
product was washed in running water until the pH was neutralized.
The alkaline extraction of basidiomycetes was performed with a 1% sodium
hydroxide solution (1:10) at a temperature of 30°C (oil bath) for 3-4 hours. The
extract was filtered through a nylon cloth, centrifuged, and neutralized with
acetic acid to a pH of 7.5. The extract was evaporated in a rotating film
evaporator at 50°C. It was then dialyzed, frozen, and dried [2]. The food is
washed in running water until the pH is neutralized
The alkaline portion of basidiomycetes was treated with a 1% sodium
hydroxide solution (1:10) at a temperature of 30°C (oil bath) for 3-4 hours. The
extract was filtered through a nylon cloth, centrifuged, and neutralized with
acetic acid to a pH of 7.5. The extract was evaporated in a rotating film
evaporator at 50°C. It was dialyzed, frozen, and dried.[2]
Determining the solubility of the sample. The weighted portion of the
samples weighing 0.5 g was placed in a 100 ml conical flask and filled with 50 ml
of distilled water. The flask is shaken for 2 hours, then the solution is filtered
through a Schott filter (the pore size is 160 μm, previously adjusted to constant
weight). After immersion, the filter cake was dried and pulled together with the
Shota filter. The solubility is calculated by the following formula:[3]
%;
100
)
(
(%)
lub
1
2
3
1
m
m
m
m
ility
so
where m1- is the mass of the sample, g; m2 -is the mass of the empty filter,
g; m3- is the mass of the filter with the sediment, g.
Table 1.
Methods for isolating beta-glucan polysaccharides and their influence
on the
monomeric composition
Overal
Ara
Xyl
Glc
Ara/xyl
Glc/Ara
Glc/Xyl
Glc/AX
GAX/GA
Salt wat.
54,4
18,2 28,4 7,8
0,64
0,43
0,27
12,17 28,4
Dist.wat.
49,8
4,8 7,3 37,7 0,66
7,85
5,16
57,34 7,3
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KOH
31,6
5,8 12,3 13,5 0,47
2,33
1,10
28,63 12,3
NaOH
15,1
2
4
9,1
0,50
4,55
2,28
18,20 4
SEF
9,8
2,30 2,20 5,30 1,05
2,30
2,41
5,07 2,2
Table shows that the solubility of isolated samples is significantly
influenced by the type of monomeric glucoside units and their ratio. It is evident
that among these units, the ratio of Arabinose and Xylose units to glucose units
is the main factor influencing the water solubility factor. When this value was
57.34, the solubility of the samples in distilled water was the highest, reaching
12.5%.
Figure 1. Diagram of the influence of beta-glucan polysaccharide monomer
composition on water solubility
References:
1.
Alves M. J.; Ferreira, I. C.; Dias, J.; Teixeira, V.; Martins, A.; Pintado, M. A.
(2012). Review on Antimicrobial Activity of Mushroom (Basidiomycetes)
Extracts and Isolated Compounds // Planta Medica 78(16):1707-1718. doi:
10.1055/s-0032-1315370
2.
Xalilova G.A., Muxitdinov B.I., Xaytmetova S.B., Azimova L.B.,
Normaxamatov N.S., Turaev A.S. Vыdelenie vodorastvorimыx polisaxaridov iz
bazidialnыx gribov, izuchenie ix sostava i usloviy ekstraksii//
Farmasevticheskiy jurnal. 2019. No4. S.72–83.
3.
Ye T.et al. The pathway by which the yeast protein kinase Snf1p controls
acquisition of sodium tolerance is different from that mediating glucose
0,00
10,00
20,00
30,00
40,00
50,00
60,00
70,00
0
1
2
3
4
5
6
Ara/xyl
Glc/Ara
Glc/Xyl
Glc/AX
GAX/GA
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regulation // Microbiology. 2008. Vol. 154.Pp.2814–2826. DOI:
10.1099/mic.0.2008/020149-0.
4.
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