Mualliflar

  • Z.R. Askarova
  • Tagaibaeva Asem Kairat kizi

Muallif tarjimai holi

  • Z.R. Askarova

    Chirchik State Pedagogical University (CSPU),

     

  • Tagaibaeva Asem Kairat kizi

    Chirchik State Pedagogical University (CSPU),

DOI:

https://doi.org/10.71337/inlibrary.uz.yottoro.90458

Kalit so‘zlar:

Key words: Fusarium identification fungi mycotoxins fusarium phytopathogen morphological method molecular methods PCR DNA sequencing microscopy plant pathogens food safety fusarium diseases fumonisins.

Annotasiya

Abstract: Fungi of the genus Fusarium are an important group of microscopic fungi that play a significant role as plant pathogens and sources of toxins hazardous to human and animal health. These fungi are widespread in soil and plant debris, causing diseases of various agricultural crops, such as fusarium, and causing food spoilage. Some Fusarium species can produce dangerous mycotoxins such as fumonisins and trichothecenes, making them important targets for study in the field of food safety.


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IDENTIFICATION OF FUNGI OF THE GENUS FUSARIUM

1

Z.R. Askarova,

1

Tagaibaeva Asem Kairat kizi

1

Chirchik State Pedagogical University (CSPU),

Abstract: Fungi of the genus Fusarium are an important group of

microscopic fungi that play a significant role as plant pathogens and sources of

toxins hazardous to human and animal health. These fungi are widespread in soil

and plant debris, causing diseases of various agricultural crops, such as fusarium,

and causing food spoilage. Some Fusarium species can produce dangerous

mycotoxins such as fumonisins and trichothecenes, making them important targets

for study in the field of food safety.

Some Fusarium species can produce dangerous mycotoxins such as

fumonisins and trichothecenes, making them important targets for study in the field

of food safety.

Identification of Fusarium fungi is of key importance for plant disease

diagnosis and food and feed contamination monitoring.

Key words: Fusarium, identification, fungi, mycotoxins, fusarium,

phytopathogen, morphological method, molecular methods, PCR, DNA

sequencing, microscopy, plant pathogens, food safety, fusarium diseases,

fumonisins.

Introduction:

In 1809, the German scientist H.F. Link first united many

different species of fungi into the genus

Fusarium.

At the first stages, the

classification of this genus was carried out only on the basis of superficial

observations, while the cultural characteristics of the studied samples were

practically not taken into account. Based only on such observations, more than

1,000 species, varieties and forms of

Fusarium

were classified. Most species of the


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genus

Fusarium

are primarily plant pathogens that cause wilt, developing in the

vascular system of plants; damage to the ear, fruit and seeds; rot of vegetative and

generative parts of plants; root rot.

Fusariums

damage various important

agricultural crops: cereals (especially wheat, barley, corn), fruits, vegetables,

ornamental crops and others [2].

Fungi of the genus

Fusarium

can remain in the soil for a long time, feed on

organic matter in the soil and plant residues, and after a certain period penetrate

healthy plants. In addition, they have the ability to infect plants belonging to

different families, but growing in the same conditions [3].

Fungi of the genus

Fusarium

in the conditions of the Tashkent region affect

annual, biennial, perennial, herbaceous, woody, cultivated and wild plants, a total

of 70 species belonging to 20 families. Pathogenic species of the genus

Fusarium

affect the above-ground and underground parts of plants, and lead to rotting of

stems, wilting of leaves, rotting of seeds, root crops, tubers, fruits [3].

Diseases of agricultural plants caused by fungi cause significant damage to

crops and lead to economic losses. For the timely application of plant protection

products against diseases and control of grain contamination by phytopathogenic

fungi at different stages of its production and processing, their detection and

accurate identification are necessary [4].

Fungi of the genus

Fusarium

are capable of infecting plants and producing

mycotoxins in a wide range of temperatures. One of the leading factors in the

development of the disease is excess humidity of more than 71%, especially during

the flowering period. The negative impact of fusarium on plants is expressed in the

suppression of photosynthesis, immunity, enzymes, which leads to the death of the

plant. [5].

Research methods:

The danger posed by fungi of the genus

Fusarium

and

the mycotoxins they produce makes it necessary to develop methods for rapid and

reliable determination of the species affiliation of strains, which will make it

possible to determine the spectrum of compounds accumulating in a crop or batch


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of grain. Today, methods based on DNA polymorphism analysis play an important

role in taxonomic studies of the genus

Fusarium

and identification of its

representatives. The use of a molecular genetic approach has made it possible to

more clearly establish standards and boundaries of species, as well as to

characterize a number of new taxa. In addition, the study of inter- and intraspecific

DNA polymorphism has made it possible to develop a number of highly specific

systems for the diagnosis and identification of the main pathogens of

fusarium

,

primarily based on PCR and its modifications. The use of modern molecular

biological and bioinformatics methods, including whole-genome sequencing, has

significantly accelerated the study of the genetic diversity of the genus

Fusarium

and the functional characterization of genomic elements, but the search for

effective methods of molecular labeling and informative DNA barcodes remains

relevant [1].

Conclusion:

The combination of morphological, molecular and

biochemical methods enables accurate identification of

Fusarium

fungi, which is

important for understanding their ecology, role in plant diseases and food safety.

In-depth studies will help improve pathogen control, reduce mycotoxin risks and

increase agricultural productivity.

Literature

1.

A. A. Stakheev, L. V. Samokhvalova, O. D. Mikityuk, S. K. Zavriev.

Phylogenetic analysis and molecular typing of trichothecene-producing

fungi of the genus

Fusarium

from Russian collections. VOL. 10 No. 2 (37)

2018 | ACTA NATURAE | 99.

2.

2. A. A. Shvartsev, M. L. Koroleva, S. A. Blinova, V. S. Rubets, V. V.

Pylnev, Molecular genetic analysis for

Fusarium

species diversity in the

winter triticale (×Triticosecale Wittm. ex A. Camus), Bulletin of Perm

University. Biology. 2023. Iss. 1. P. 65‒75.


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3.

A.G.Sherimbetov. IDENTIFICATION OF THE SPECIES OF FUNGI OF

THE GENUS

FUSARIUM

AFFECTING SWEET PEPPER PLANTS

(CAPSICUM

ANNUUM

L.),

INTERNATIONAL

SCIENTIFIC

JOURNAL "MODERN BIOLOGY AND GENETICS" 2024 No. 1 (7)

4.

S. L. Abramova, Identification of wheat rust pathogens by polymerase chain

reaction / S. L. Abramova, A. I. Zhemchuzhina, N. S. Zhemchuzhina, D. Yu.

Ryazantsev, S. K. Zavriev // Bulletin of the Russian Academy of

Agricultural Sciences. - 2011. - No. 5. - P. 15-17.

5.

T.A. Nugmanova, M.V. Kabargina. FUSHI GENERUS

F U S A R I U M

MULTIPLE PROPERTIES, IMPORTANCE OF AS A OBJECT IN

INDUSTRIAL BIOTECHNOLOGY. LLC "BIOIN-NOVO", Moscow,

Russian Federation. 2024.

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