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LEVELS OF EXPRESSION OF CD31+, CD3+, CD20+ IMMUNOHISTOCHEMICAL
MARKERS IN ADRENAL GLAND TISSUE IN COVID-19
Najmiddinova Nilufar Nurali kizi
Republic Pathological Anatomy Centre
PhD researcher +998977521525
nilya_92@mail.ru
https://doi.org/10.5281/zenodo.13989532
Abstract.
CD31+, CD3+, CD20+ immunohistochemical markers were studied in the
adrenal gland tissue in order to study the degree of damage to the adrenal gland caused by
COVID-19 infection and the immunological response of the organ against the virus.
Keywords:
Adrenal glands, immunohistochemistry, antidiv, cortex, expression.
The purpose of the study:
To study immunohistochemical changes in cortex of adrenal
glands of patients who died of COVID-19 infection.
Materials and methods:
The object of the study was the examination and performance
of immunohistochemical procedures of isolated adrenal glands during autopsy from 50
patients who died from COVID-19 infection at the Republican Pathological Anatomy Center.
Research results:
CD31+ protein is a molecule that adheres to endothelium and
platelets. To study the expression of CD31+ protein under the influence of monoclonal
antidiv, 3 morphofunctional areas of the adrenal cortex were studied separately. In our
study, it was observed that all morphofunctional areas of the adrenal cortex were expressed
in more than 50% of endothelial cells in the vascular wall. In this case, it was found that it was
expressed at the level of 86.5% in glomerulosa zona, 81.4% in fascicular zona, and 79.2% in
the reticular zona, and the vessel wall was completely painted in dark brown color. The
medium expression level was found to be 11.5% in glomerulosa zona, 14.8% in fascicular
zona, and 15.7% in reticular zona. Low-level expression was found to be 2.0% in glomerulosa
zona, 3.8% in fascicular zona, and 5.1% in the reticular zona.
In almost all the morphofunctional areas of the adrenal gland cortex that we studied, it
was found that the high level of expression of the CD3+ marker was very low in 50% of the
cells, it was observed that it was expressed at the level of 1.5% in glomerulosa zona, 2.7% in
fascicular zona, and 4.5% in the reticular zona. Medium expression of the CD3+ marker was
8.6% in the glomerulosa zona, 9.5% in fascicular zona, and 7.5% in the reticular zona.
It was found that the CD20+ marker was expressed at the level of 1.2%, 3.7%, and 6.5%
accordingly in adrenal cortex. Moderate expression of this marker was observed with 2.6%
expression in glomerulosa zona and 8.5% expression in fascicular and reticular areas.
Conclusions
. Since the CD31+ marker is present on the endothelial cells of the inner
surface of vessels and on almost all white blood cells in the blood, in our study, all
morphofunctional areas of the adrenal cortex were expressed at a level higher than 50% of
the endothelial cells in the wall of the blood vessels, including 86.5% in glomerulosa zona,
81.4% in fascicular zona and 79.2% in the reticular area were expressed, and it was found
that the vessel wall was completely stained dark brown. The percentage of highly expressed
cells of the CD3+ marker was expressed at 1.5% in glomerulosa zona, 2.7% in fascicular zona,
and 4.5% in the reticular zona. In the case of COVID-19 infection, the immune system
decreases, due to the paralysis of B-lymphocytic immunity, it was found that the CD20+
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marker was expressed at the level of 1.2%, 3.7% in fascicular zona, and 6.5% in the reticular
zona of the adrenal cortex.
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