American Journal Of Biomedical Science & Pharmaceutical Innovation
8
https://theusajournals.com/index.php/ajbspi
VOLUME
Vol.05 Issue06 2025
PAGE NO.
8-10
10.37547/ajbspi/Volume05Issue06-02
Technology and Qualitative Analysis of Liquid Extract
Obtained on The Basis of Plant Raw Materials
Polygonum Hydropiper L. And Hypericum Perforatum L.
Yuldasheva Shakhlo Khabibullayevna
Tashkent Pharmaceutical Institute, Uzbekistan
Received:
14 April 2025;
Accepted:
10 May 2025;
Published:
12 June 2025
Abstract:
This research work is devoted to the study of the technology and qualitative analysis of obtaining liquid
extract from Polygonum hydropiper and Hypéricum perforátum plant raw materials. Percolation was used in
liquid extract extraction. Ethyl alcohol of different concentrations was used as an extract. When conducting quality
analysis, a spectrophotometric method was used.
Keywords:
Liquid extract, flavonoids, Polygonum hydropiper and Hypéricum perforátum, percolation,
spectrophotometry.
Introduction:
Drug preparations with a twisting effect,
obtained from plant raw materials, are now widely
used. Agents with a twisting effect are among the drugs
that have a local anti-inflammatory effect. They are
used in inflammatory processes of the skin and mucous
membranes [1]. Therefore, when we carried out our
research work, we selected Herba Polygoni
hydropiperis and Hypéricum perforátum from plant
raw materials with a twisting effect by chemical
composition. The Polygonum hydropiper has been
used since ancient times as a medicinal plant, with
which the Greeks and Romans, al-Khimik, used it.
Polygonum hydropiper contains glycosides, vitamins C,
K, carotene, flavanoids, sugars, organic acids,
astringent bitter compounds and other substances. In
folk medicine, tincture is used in malaria, diarrhea,
hemorrhoids and as a bleeding suppressor and
painkiller and as a medicine for various wounds [5]. The
Hypéricum perforátum product contains 10-12.8
percent additives,0.1-0.4 percent anthracene unums,
flavonoids (hyperoside, rutin, quercitrin, isocversitrin,
quercetin, myricetin, etc.), 0.1 - 03 percent essential oil,
55 mg percent carotene, 1151.8 mg percent vitamin C,
~4 mg percent choline, very small amounts of alkaloids,
and up to 10 percent tar. Hypéricum perforátum
products, on the other hand, have anti-inflammatory,
spasmolytic analgesic, antiseptic and twisting effects.
In medicine, it is used in the treatment of
gastrointestinal (colitis, diarrhea), oral diseases
(gingivitis and stomatitis) and burns of II and III degrees,
as well as for rinsing the mouth. The plant surface of
the plant has a bactericidal effect [1,4].
The purpose of the work
. Obtaining liquid extract from
Polygonum hydropiper and Hypéricum perforátum
plant raw materials and conducting its qualitative
analysis is the goal of our research work.
METHODS
When
obtaining
a
liquid
extract,
on
the
recommendation of pharmacologists from the
following selected plant raw materials, herba Polygoni
hydropiperis and herba Hypérici perforátum in the
same proportion were pulled out and ground at a level
of 3-5 mm spruce. Two different capacities of ethyl
alcohol were selected as an extract (40% and 70%). The
percolation method was used in obtaining liquid
extract [2,3]. Liquid extracts were studied on the basis
of methods given in their appearance, dry residue,
alcohol concentration, density, heavy metal content
O’zR DF, XI DF [4,5]. Quantitative analysis of the
resulting liquid extracts was analyzed in a
spectrophotometric way by the average amount of
flavonoids (in the calculation of the rutin standard
substance) [2,4].
American Journal of Applied Science and Technology
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American Journal of Applied Science and Technology (ISSN: 2771-2745)
As standard, rutin flavanoid is used, in which the
maximum absorption corresponds to the maximum
absorption of the sample being examined with the
aluminum chloride complex. Measurements of optical
density were carried out on the UB-spectrophotometer
"Agilent Technology-8253" (Germany), at a wavelength
of 350-450 nm, in cuvettes 10 mm thick.
Preparation of the probing solution. 1ml of the probing
liquid extract was placed in a 25ml volume measuring
flask, 5ml of 96% ethyl alcohol, 5ml of 5% aluminium
chloride was released from a 70% solution of ethyl
alcohol. After 10 minutes, a solution of 2 ml of 5% acetic
acid in 70% ethyl alcohol was added. The volume of the
solution was reached and mixed with 70% ethyl alcohol
to the line of the measuring flask. The optical density of
the solution obtained after 30 minutes was measured
in a 10 mm thick cuvette at a wavelength of 408nm on
a spectrophotometer. A sample of a compensatory
solution was prepared-this is a solution of 1 ml of the
liquid extract, 5 ml of 96% ethyl alcohol and 2 ml of 5%
acetic acid in 70% ethyl alcohol, put in a 25 ml flask and
brought to the line of the measuring flask with 70%
ethyl alcohol.
Working standard sample solution preparation (WSS).
Approximately 25 mg (exact drawer) of WSS rutin is
obtained, dried to a constant weight at 135°C, placed in
a 100 ml volume flask, dissolved in heated 80 ml of 96%
ethyl alcohol, then cooled and transported to the line
of the measuring flask with that 96% ethyl alcohol and
mixed.
At the same time, after 30 minutes, the optical density
of 1 ml of a comparable solution (isn rutin) is measured
using a sample of a compensatory solution (1 ml of
liquid extract, 5 ml of 96% ethyl alcohol and 2 ml of 5%
acetic acid in 70% ethyl alcohol, put in a 25 ml flask and
brought to the line of the measuring solution flask with
70% ethyl alcohol).
The sum of flavanoids contained in the liquid extract is
found through the following formula:
10000
1
25
100
100
25
1
0
0
0
0
=
=
A
P
m
A
A
P
m
A
X
in this:
А —
is the optical density of a probing solution;
А0 —
is the optical density of a comparable solution;
m0
—
is the mass of the routine standard sample, mg;
Р —
The amount of rutin on the WSS, indicated in the
quality certificate, is %.
RESULTS AND DISCUSSION
Qualitative analysis of the obtained liquid extracts was
studied on the basis of
the methods given in O’zR DF, XI
DF. In this case, a liquid extract obtained using 40%
ethyl alcohol looked like this in appearance: a clear
liquid of dark green, brown color; a dry residue of
2.76%, an alcohol concentration of 35%, a density of
0.94 g/ml, the amount of heavy metals did not exceed
0.001%. The liquid extract obtained using 70% ethyl
alcohol looked the following in appearance: a clear
liquid of dark green, brown color; a dry residue of
2.22%, an alcohol concentration of 67.5%, a density of
0.92 g/ml, the amount of heavy metals did not exceed
0.001%. The results are shown in Table 1.
Liquid extract quality indicators
Table 1
№
Appearance
Alcohol
concentrate
Amount of dry
residue
Density
Total amount
sum of
flavonoids
Liquid extract
(40% ethyl
alcohol)
clear liquid of
dark green,
brown color
35%
2,76
0,94
2,3085%
Liquid extract
(70% ethyl
alcohol)
clear liquid of
dark green,
brown color
67,5%
2,22
0,92
2,5796%
Quantitative analysis of the resulting liquid extracts
was analyzed by spectrophotometry method in terms
of the average amount of flavonoids (in the standard
rutin calculation). Based on the results of a quantitative
analysis, the average amount of flavonoids in liquid
extract obtained using 40% ethyl alcohol (in standard
rutin accounting) was 2.3085%; the average amount of
flavonoids in liquid extract obtained using 70% ethyl
alcohol (in standard rutin accounting) was 2.5796%.
CONCLUSION
Liquid extracts from Polygonum hydropiper and
Hypéricum perforátum plant raw materials with the
participation
of
an
extragent
of
different
concentrations were obtained by percolation and
American Journal of Applied Science and Technology
10
https://theusajournals.com/index.php/ajast
American Journal of Applied Science and Technology (ISSN: 2771-2745)
qualitative analysis of it was carried out. According to
the results, a liquid extract obtained using 70% ethyl
alcohol was found to be optimal.
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