Volume 04 Issue 01-2024
113
American Journal Of Biomedical Science & Pharmaceutical Innovation
(ISSN
–
2771-2753)
VOLUME
04
ISSUE
01
P
AGES
:
113-117
SJIF
I
MPACT
FACTOR
(2021:
5.
705
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(2022:
5.
705
)
(2023:
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OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
ABSTRACT
The article presents the results of a study of biologically active substances in a dry extract obtained from the flower
buds of the medicinal plant saffron Crocus sativus L. The GSX method was developed to determine the amount of
biologically active substances in the dry extract.
KEYWORDS
Dry extract, saffron, chromatography, standard samples.
INTRODUCTION
Research Article
QUANTITATIVE
DETERMINATION
OF
BIOLOGICALLY
ACTIVE
COMPOUNDS FROM DRY EXTRACT OF SAFFRON (CROCUS SATIVUS L.)
BY GAS CHROMATOGRAPHY
Submission Date:
January 21, 2024,
Accepted Date:
January 26, 2024,
Published Date:
January 31, 2024
Crossref doi:
https://doi.org/10.37547/ajbspi/Volume04Issue01-17
M.O.Shomaksudova
Tashkent Pharmaceutical Institute, Uzkfiti, Institute Of Bioorganic Chemistry Of The Academy Of Sciences Of
The Republic Of Uzbekistan
A.A.Tulaganov
Tashkent Pharmaceutical Institute, Uzkfiti, Institute Of Bioorganic Chemistry Of The Academy Of Sciences Of
The Republic Of Uzbekistan
A.D.Matchanov
Tashkent Pharmaceutical Institute, Uzkfiti, Institute Of Bioorganic Chemistry Of The Academy Of Sciences Of
The Republic Of Uzbekistan
D.M.Sarvarova
Tashkent Pharmaceutical Institute, Uzkfiti, Institute Of Bioorganic Chemistry Of The Academy Of Sciences Of
The Republic Of Uzbekistan
Journal
Website:
https://theusajournals.
com/index.php/ajbspi
Copyright:
Original
content from this work
may be used under the
terms of the creative
commons
attributes
4.0 licence.
Volume 04 Issue 01-2024
114
American Journal Of Biomedical Science & Pharmaceutical Innovation
(ISSN
–
2771-2753)
VOLUME
04
ISSUE
01
P
AGES
:
113-117
SJIF
I
MPACT
FACTOR
(2021:
5.
705
)
(2022:
5.
705
)
(2023:
6.534
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
Chromatography makes it possible to carry out
qualitative and quantitative analysis of the objects
under study, study the physical and chemical
properties of substances, and monitor and
automatically regulate technological processes. Gas
chromatography is a method for separating volatile
compounds.
This method can analyze gaseous, liquid and solid
substances with a molecular weight of less than 400
that satisfy certain requirements, the main ones being
volatility, thermal stability, inertness and ease of
preparation. Quantitative analysis can only be carried
out if the substance is heat-resistant, i.e. evaporates
reproducibly in the dispenser and elutes from the
column without decomposition (1,7).
In pharmaceutical analysis, the GLC method is
widely used to determine residual organic solvents in
pharmaceutical
substances,
biological
active
substances in liquid and dry extracts of medicinal plant
materials, as well as in assessing the quality of essential
oils, volatile medicinal substances, and their
preparations (2,5,6).
The purpose of our work is to study the
quantitative content of biological active substances in
a dry extract obtained from plant raw materials of
saffron using gas-liquid chromatography. Previously,
we studied the qualitative content of biologically active
substances in plant raw materials of saffron using gas
chromatography-mass spectroscopy (3).
For the quantitative determination of
biologically active substances in the dry extract of
saffron, we used the dry extract we had previously
obtained by the percolation method (4).
Experimental parts.
Quantification method:
Gas chromatograph
–
Khromatek-Kristall
–
5000 (Russia)
Detector-FID
Detector temperature
–
250 0С
Thermostat temperature (gradient) -
70 0С (6min), 1500С (6
-
15min), 200 0С (15
-
33min), 220 0С (33
-38min)
Injector temperature -
200 0С
Carrier gas
–
nitrogen
Split flow
–
1/20
Injection
–
1 µl
Volume 04 Issue 01-2024
115
American Journal Of Biomedical Science & Pharmaceutical Innovation
(ISSN
–
2771-2753)
VOLUME
04
ISSUE
01
P
AGES
:
113-117
SJIF
I
MPACT
FACTOR
(2021:
5.
705
)
(2022:
5.
705
)
(2023:
6.534
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
Fig. 1 Chromatogram of standard samples
Figure 1 shows chromatogram data for the three main standard substances isophorone with a retention time of 26.777
min, 2,6,6-trimethylcyclohexa-1,3-diene-1-carbaldehyde with a column retention time of 28.783 min, and 4-
ochophorone with a retention time of 30.916 min . For comparison, the concentration of standard samples was 3
mg/ml.
Fig 2 Chromatogram of hexane extract of dry extract of Saffron
Volume 04 Issue 01-2024
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American Journal Of Biomedical Science & Pharmaceutical Innovation
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VOLUME
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Publisher:
Oscar Publishing Services
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From the data presented in Fig. 2, it is clear that in the hexane extract of the Saffron plant all three main components
are observed: isophorone with a retention time of 26.766 minutes, 2,6,6-trimethylcyclohexa-1,3-dien-1-carbaldehyde
with a retention time of column 28.751 min and 4-ochophorone with a retention time of 30.875 min. The quantitative
content of isophorone is 0.76 mg/ml, 2,6,6-trimethylcyclohexa-1,3-diene-1-carbaldehyde is 5.71 mg/ml and 4-
ochophorone is 0.68 mg/ml.
Fig 3 Chromatogram of acetonitrile extract of dry extract of Saffron
From the data presented in Fig. 3, it is clear that in the
acetonite extract of the Saffron plant two main
components are observed: 2,6,6-trimethylcyclohexa-
1,3-diene-1-carbaldehyde with a retention time on the
column of 28.745 minutes and 4-ochophorone with
retention time 30.877 min. The quantitative content of
2,6,6-trimethylcyclohexa-1,3-diene-1-carbaldehyde
is
0.44 mg/ml and 4-ochophorone is 0.42 mg/ml.
Conclusion. Thus, studies have shown that for the
extraction of Saffron it is relatively more effective to
use hexane as a solvent.
REFERENCES
1.
Е.А. Кривошеев, Р.И. Саяхов. «Газовая
хроматография» Методические указания к
лабораторной работе Казань, 2021. 23 с. ВВ.
2.
В.
Тыжигирова
«Газовая
хроматография»Краткая
характеристика
метода
и
его
применение
в
фармацевтическом
анализе
Учебное
пособие Иркутск ИГМУ 2016
3.
М.О.
Шомахсудова,
А.А.
Тулаганов,
Я.К.Назирова, С.З. Нишанбаев. «Разработка
Volume 04 Issue 01-2024
117
American Journal Of Biomedical Science & Pharmaceutical Innovation
(ISSN
–
2771-2753)
VOLUME
04
ISSUE
01
P
AGES
:
113-117
SJIF
I
MPACT
FACTOR
(2021:
5.
705
)
(2022:
5.
705
)
(2023:
6.534
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
методов анализа биологически актывних
веществ в растительном сыре шафрана
посевного(Crocus sativus L) с помощью
хромато
-
спектрометрии»,
Pharmaceutical
journal, №2, том.29, 2020 Тошкент.
4.
Шомаксудова М.О., Тулаганов А.А. .XI
Международной
научно
-
практической
конференции. “Современная фармация
новые падходы в образовании актуальные
исследования ”,приуроченной к 20
-
летию
факультета Фармация. 5май 2022й Нур
-
Султан.
5.
Zuparova, Z. A., Olimov, N. K., Ismoilova, G. M.,
& Khasanova, B. J. (2020). Determination of
high quality of echinaceae purpurae herba
grown in Uzbekistan and the prospect of
creating
immunomodulatory
medicinal
products on its base. International Journal of
Psychosocial Rehabilitation, 24(4), 2355-2366.
6.
Zuparova, Z. A., and G. M. Ismoilova.
"Determining The Authenticity Of Immunacea
Bio Tablets With Immunomodulatory Action."
Journal of Pharmaceutical Negative Results
(2022): 2637-2641.
7.
Sarvarova, D. M., et al. "Study Of The
Assortment Of Antioxidant And Hemostatic
Medicines Registered In The Republic Of
Uzbekistan."
Journal of
Pharmaceutical
Negative Results (2023): 1028-1033.
