MORPHOLOGICAL CHARACTERISTICS OF THE UTERUS OF WHITE OUTBRED RATS

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ABSTRACT

Using light microscopy methods, the histological structure of the uterus of white outbred rats at the level of the div

and cervix was clarified. It has been established that the walls of the right and left uterine horns grow together,

forming a double div and neck. Whose cavities remain separated from each other by a thin septum and open into

the vagina with two separate openings. The area of fusion of the medial walls is characterized by the union of the

perimeter and the supravascular layer of the myometrium. The data obtained can be used to illuminate the relevant

sections of reference and educational literature when writing sections on evolutionary, functional, comparative

species morphology and reproductive biology. Based on the histological study, it was established that in rats, despite

the general type of uterine structure, there are significant differences that mainly affect their lower segment at the

borders.

KEYWORDS

Uterus, cervix, white outbred rats, myometrium, smooth myocyte.

INTRODUCTION

Research Article

MORPHOLOGICAL CHARACTERISTICS OF THE UTERUS OF WHITE
OUTBRED RATS

Submission Date:

January 01, 2024,

Accepted Date:

January 05, 2024,

Published Date:

January 08, 2024

Crossref doi:

https://doi.org/10.37547/ajbspi/Volume04Issue01-04

Ishankulova Dildora Xabibullayevna

Bukhara State Medical Institute, Uzbekistan

Ixtiyarova Gulchehra Akmalovna

Bukhara State Medical Institute, Uzbekistan

Ilyasov Aziz Saidmuratovich

Bukhara State Medical Institute, Uzbekistan

Journal

Website:

https://theusajournals.
com/index.php/ajbspi

Copyright:

Original

content from this work
may be used under the
terms of the creative
commons

attributes

4.0 licence.

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The study of animal templates, the development of

animals and the methods of their management, their

reproduction processes, are some of the pressing

problems of the time. Basic knowledge shows the

structure of laboratory animals, and we can

understand the mechanisms of differentiation,

abnormal histo - and Organogenesis, as well as

contribute to greater depth and purpose. A better

understanding of morphological bases, relationships

between tissues during development, renewal, and

restoration can help study multi-tissue systems. From

laboratory animals, due to the onset of the most

favorable and rapid puberty for a century and a half,

the short duration of embryogenesis and the short

period of postpartum stretching involution are rats and

rabbits. The presence of animal material was achieved

to ensure the purity of the samples. An analysis of the

literature shows that there are many unanswered

questions related to the rest the structure of some

organs and organ systems is new, in particular, it

concerns the genital area; that is, the uterus is the

sections of its caudal parts associated with the

formation of the div and neck at the level of

insertion. There are also many unresolved problems,

related to the structure of its caudal part, and

especially its appearance at the microscopic level. The

smooth muscle is still not well understood. Myometrial

tissue in the caudal part of the uterus, as you know, is

characterized by originality. What functions it

performs and does it to the maximum extent during

endocrine dependence, pregnancy and childbirth. The

practical significance of this information can be useful

for

biologists,

veterinarians

and,

perhaps,

Obstetricians-Gynecologists, I think.

Taking into account the above, the purpose and

comparative analysis of the study, with the

histostructural Organization of the uterine wall of the

rat, its caudal part was taxied in modern histo-

cytological methods.

Research objective. Expanding knowledge of the

morphological structure of the female genital organs,

in particular the uterus in rats.

MATERIAL AND METHODS

The work used 15 bats ranging from 4 months to 1 year.

The uterus was used as an object of study. The Material

was ivitized to a 10% buffered formalin solution. The

placement of the material was carried out on a

histological closed-type vacuum Leica ASP 300

processor. The material used Histomix paraffin from

the BioOpticamiz firm. The cuts were made in a rotor

Microtome 6-7 µm thick. The work used methods of

light microscopy, painting cuts with hematoxylin and

eosin dye. Immunogystochemical testing of caudal

tissue was also performed. The ratio of the uterine

section to actin in smooth muscles was carried out

using a set of monoclonal antitanours. The

establishment of an immunogystochemical reaction

was achieved with a one-step imaging system.

Results and analyzes. The squid uterus contains a pair

of horns, div and neck, and the uterine horns join in a

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caudal direction to form the div and neck, but their

cavities are separated by a barrier and open with two

independent holes in the vagina. It should be noted

that in basic education, veterinary and monographic

applications in development biology in rats, according

to the basic classification of bacadon, the uterus is

defined as bipartite, that is, the Union of bodies to

form a single cervical hole. With complex study, this

question becomes clear that the uterus should be

classified as two-horned the uterus of rats.

A histological examination found that the wall of the

branches, div and cervix has a similar structure and

consists of three walls: endometrium (mucous

membrane), myometrium (muscle) and perimetry

(serosis).

(Figure 1). Horn and glands of the uterus of a white broodless rat. Coloring: with hematoxylin and eosin.

The mucous membrane forms a relief in the form of folds that become more pronounced on the neck (fig. 2). The

formed endometrium forms in two layers (plates): epithelial and specific.

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(fig. 3) the cervix and glands of a white broodless rat. Coloring: with Van Gison.

(fig. 4) the cervix and glands of a white broodless rat. Coloring: with Van Gison.

Thus, in the lower segment, a median border is formed

that separates the uterus from the two div and

cervical cavities, which are vaguely expressed. The

middle border is made up of endometrium and

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myometrium. At the same time, myometrium has

submucosal and single vascular layers in its structure.

The vascular layer is more expressed in the div.

CONCLUSIONS

Histo-morphological studies conducted are considered

important, and its results can be used in reference and

educational literature and reproduction biology when

writing evolutionary, functional, relative species

morphology sections to write relevant sections. This

may allow a comparative study of the structure of the

female genitalia and the observation of their

homology. In the development of various mammals,

people, revealing their evolutionary dynamics, is of

great importance. Thus, in rats, the cervix has more in

common with humans, which reveals in advance what

should be considered when looking for an

experimental model when conducting research.

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