ACADEMIC RESEARCH IN MODERN SCIENCE
International scientific-online conference
87
EFFECT OF THE PROBIOCOR M PROBIOTICS ON
MORPHOBIOCHEMICAL INDICATORS OF RABBIT BLOOD
Eshmatov G‘.
Scientific supervisor - Associate Professor -
G‘aniyev S.
Student
Tashkent branch of Samarkand State University of
Veterinary Medicine, Animal Husbandry and Biotechnology
https://doi.org/10.5281/zenodo.15305010
Abstract.
This article studies the effect of the ProBioKorm probiotic on the
div of rabbits, in particular on the morphobiochemical parameters of their
blood. In the experiment, a certain amount of ProBioKorm probiotic was
administered to the experimental group of rabbits, and their blood samples
were monitored for erythrocytes, hemoglobin, leukocytes, as well as total
protein and other basic biochemical parameters.
Keywords:
ProBioKorm, probiotic, hemoglobin, erythrocyte, leukocyte,
morphobiochemical, indicators, blood
Relevance of the topic.
In recent years, the issue of using
environmentally friendly, effective and safe biological additives in animal
husbandry, in particular in rabbit breeding, has become relevant. Against the
background of restrictions on antibiotics and their negative consequences, the
need for probiotics is increasing significantly. Probiotics play an important role,
in particular, in strengthening the immune system of animals, improving
digestion and strengthening general health. One of such tools is the ProBioKorm
probiotic, the scientific study of its effect on the morphological and biochemical
parameters of rabbit blood is of great importance in determining the
effectiveness of probiotics. The results of this study serve as an important
scientific and practical basis for the formation of innovative approaches aimed at
raising healthy and highly productive animals in rabbit breeding.
Main part.
ProBioKorm is a probiotic agent developed for animals, which
contains a complex of beneficial bacteria. Morphological (for example,
erythrocytes, leukocytes, hemoglobin) and biochemical (total protein) indicators
of the blood of rabbits are the main criteria for determining their health status.
By studying the effect of the ProBioKorm preparation on these indicators, it is
possible to assess the effectiveness of the preparation and determine the
possibilities of its practical application. The feed additive "ProBio K orm" was
developed by employees of the Institute of Microbiology of the Academy of
Sciences of the Republic of Uzbekistan in order to improve and enrich the
ACADEMIC RESEARCH IN MODERN SCIENCE
International scientific-online conference
88
quality of animal feed, improve digestion and absorption of nutrients, prevent
various bacterial diseases in animals, and increase productivity. The universal
probiotic feed additive “ProBioKorm” for livestock is intended for feeding farm
animals, in particular rabbits and poultry. In addition to being a feed additive,
the universal probiotic “ProBioKorm” can also be used to treat various diseases
in animals and increase their productivity. Tables 1 and 2 below present the
analysis of the GOST physical, chemical, and microbiological special tests of the
domestic universal probiotic “ProBioKorm”.
Classification of probiotics "ProBioKorm"
organoleptic, physicochemical and safety indicators
Table 1
Indicator name
Descriptions and standards
Control
methods
Liquid form
Dry form
1 Appearance
Liquid mass
Free-flowing
powder
Visually
2 Color
Cream, light brown
Visually
3 Smell
Pure milky, without foreign
tastes or odors
Organolept
ically
4 Solubility index, min,
max
Unregulated
2 - 7
GOST
30305.4
5 Humidity, %, not more
than
Unregulated
Up to 5
GOST
24061
6
Hydrogen
ion
concentration, pH, at the
limit
3.5 -7.0
Unregulated
GOST
26188
7 The number of useful
viable cells, CFU/ml, not
less than
1.109
1.109
GOST
10444.11
8 Microscopy:
Gram-positive rods, granular,
thin, densely packed, single or in
chains, diplococci
GOST 9225
9
Escherichia
coli
bacteria
(coliforms),
CFU/g, not more than
10.0
2.0
GOST
30518
10
Staphylococci
(
Staphycoccus aurens), K
10.0
2.0
GOST
10444.2,
ACADEMIC RESEARCH IN MODERN SCIENCE
International scientific-online conference
89
CFU/g, not much
GOST
30347
11
Pathogenic
microorganisms
,
including salmonella ,
CFU / g, not more than
50.0
10.0
GOST
10444.5,
GOST
10444.15,
GOST
30519
12 Yeast count, CFU/g,
not more
10.0
10.0
GOST
10444.12
13 Mold count , K CFU/g,
not more than
10.0
10.0
GOST
10444.12
Special bacterial strains contained in the feed additive “ProBioKorm”
Table 2
Microorganisms and their strains present in the dry and liquid form of
the probiotic “ProBioKorm”
1.
- Bacillus subtilis;
2.
- Lactobacillus plantarum;
3.
- Lactobacillus paraplantarum.
4.
- Lactobacillus acidophilus;
5.
- Pediococcus pentosaceus;
6.
- Weissella viridescens;
7.
- Propionibacterium freudenreichii;
8.
- Bifidobacterium animalis;
9.
- Saccharomyces cerevisiae.
Work progress
: A total of 20 3–4-month-old female rabbits of the New
Zealand White rabbit breed, which is widespread in Uzbekistan, were selected
for the study. They were divided equally into two groups: 10 control group and
10 experimental group.
During the study, all rabbits were provided with the same care and feeding
conditions. The animals were kept in special rooms with a temperature of 13–22
°C, in individual cages, and fed a healthy, balanced diet. Water and feed were
constantly freely available. The rabbits in the experimental group were
additionally given the probiotic feed supplement ProBioKorm in an amount of
2% of the daily feed.
ACADEMIC RESEARCH IN MODERN SCIENCE
International scientific-online conference
90
During the study, blood samples were taken from the rabbits 3 times:
before administration of the drug, on day 45, and on day 90.
The following morphological parameters of the obtained blood samples
were examined in laboratory conditions using classical hematological methods:
Hemoglobin (g/l), Erythrocyte count (10¹²/l), Leukocyte count (10⁹/l)
The results of the analysis are presented in the table below.
Morphological parameters in rabbit blood (M±m)
Table 3.
Indicator
Before the drug On the 45th day On the 90th day
Hemoglobin, g/l
111.0 ± 0.12
119.4 ± 0.15 *
125.0 ± 0.24 **
Erythrocytes, 10¹²/l
6.05 ± 0.06
6.33 ± 0.03 *
6.50 ± 0.08 **
Leukocytes, 10⁹/l
7.07 ± 0.15
7.5 ± 0.19
9.17 ± 0.13
Probiotic consumer goods did experience group in animals hemoglobin
and erythrocytes amount increased , especially on days 45 and 90 statistic in
terms of reliable growth record done observed ( p < 0.05 and p < 0.01). This
indicates an increase in their ability to transport oxygen through the blood. The
number of leukocytes, although not significantly changed, increased slightly
compared to day 90, indicating that this may have affected the div's immune
response.
Biochemical parameters in the blood of rabbits in the experimental group
(M±m)
Table 4.
Indicator
Before the drug On the 45th day On the 90th day
Total protein, g/l
60.21 ± 0.47
68.04 ± 0.76
69.0 ± 0.91
Albumin, g/l
34.11 ± 0.28
43.42 ± 1.28
45.3 ± 1.276
α-globulin, %
8.82 ± 0.15
8.84 ± 0.12 *
8.57 ± 0.11 **
β-globulin, %
8.86 ± 0.18
9.33 ± 0.16
9.45 ± 0.20 **
γ-globulin, %
19.48 ± 0.25
19.48 ± 0.25
19.37 ± 0.47
Calcium, mmol/l
2.26 ± 0.03
2.44 ± 0.05 **
2.44 ± 0.05 **
ACADEMIC RESEARCH IN MODERN SCIENCE
International scientific-online conference
91
Indicator
Before the drug On the 45th day On the 90th day
Phosphorus, mmol/l
0.94 ± 0.04
1.002 ± 0.03 *
1.002 ± 0.03 *
Physiological norms: total protein – 60-82 g/l; albumin - 33-53.3 g/l; α-globulin
- 8-12%; β-globulin - 7-13%; g-globulin - 17-23%; calcium - 2.12-2.68 mmol/l;
phosphorus - 0.85-1.13 mmol/l.
experimental rabbits, positive changes were observed in the biochemical
composition of blood serum. On the 45th and 90th days, the total protein and
albumin content significantly increased, which indicates the activation of protein
metabolism. In particular, a stable increase in the level of albumin confirms the
metabolic activity of the organism and the general healthy state. In addition, the
content of calcium and phosphorus increased statistically significantly (p < 0.01
and p < 0.05), which indicates a positive effect on mineral metabolism and the
active development of the bone system. A slight increase in the level of α- and β-
globulins indicates an increase in immune activity. The γ-globulin index
remained stable, indicating that the general state of the immune system was
within the norm.
Diagram of indicators that have undergone significant changes in the
experiment:
ACADEMIC RESEARCH IN MODERN SCIENCE
International scientific-online conference
92
Conclusion
: The results of the conducted scientific experiment showed
that the use of the ProBioKorm probiotic feed additive has a positive effect on
the morphobiochemical parameters of the blood of New Zealand White rabbits,
which are widespread in Uzbekistan.
These results confirm the beneficial effects of the ProBioKorm probiotic on the
div and indicate its potential use in rabbit breeding for the production of
healthy, stable physiologically stable and highly productive animals. The safety,
efficacy and ease of application of the drug allow us to recommend it as a
promising tool in modern animal husbandry.
List of used literature:
1.
Bakhtiyorovich E. S., SaifiddinJakhongirUgli K. DIAGNOSIS OF PROTEIN
METABOLISM DISORDERS IN FISH //American Journal of Agriculture and
Horticulture Innovations. – 2023. – Т. 3. – №. 05. – С. 04-12.
2.
Qosimov S. J., Sh U. K. Hypovitaminos A And D In Young Animals
//American Journal of Advanced Scientific Research. – 2025. – Т. 2. – №. 10. – С.
79-84.
3.
Eshburiyev S. B., Kasimov S. J., Aslonova M. A. Causes and symptoms of
protein metabolism disorders in fish //Proceedings of international conference
on scientific research in Natural and Social Sciences. – 2023. – Т. 2. – №. 1. – С.
55-63.
4.
Касимов С. Ж., Мамацалаева З. ИНФЕКЦИОННЫЙ БРОНХИТ ПТИЦ
//Global Science Review. – 2025. – Т. 2. – №. 1. – С. 80-83.
5.
Kasimov S. et al. The Pathomorphology Of Disorders Of Vitamins And
Protein Metabolism In Fish //The American Journal of Veterinary Sciences and
Wildlife Discovery. – 2021. – Т. 3. – №. 06. – С. 9-12.
6.
Eshmatov, G. E. (2022). Harmony of education and student devlopment in
the process of physical education.
7.
Eshmatov, G. X., Niyozov, H. B., Yusupov, O. S., & Nazarov, A. B. (2019).
Ferula assafoetida herbaceous herb morphological parameters of blood of
karakul sheep. ACADEMICIA: An International Multidisciplinary Research
Journal, 9(4), 146-151.
8.
O'g'li, E. (2019). Influence of herbaceous grain on Ferula assafoet on the
quantitative and qualitative characteristics of snowflake rams sperm. Asian
Journal of Multidimensional Research (AJMR), 8(11), 6-10.
9.
Ниязов, Х. Б. ДЕЙСТВИЕ СЕМЯН ФЕРУЛЫ НА МОРФОЛОГИЧЕСКИЕ
ПОКАЗАТЕЛИ КРОВИ И ПЛОДОВИТОСТЬ КРОЛИКОВ. Zbiór artykułów
naukowych recenzowanych., 80.
