74
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
DEVELOPMENT OF A TECHNOLOGY FOR OBTAINING DRY EXTRACT AND
ESSENTIAL OIL FROM WORMWOOD HERB
Sadenova A.A.
1
Daurenbekov K.N.
2
JSC South Kazakhstan Medical Academy, Shymkent, Republic of Kazakhstan
e-mail: nuri_2011_1983@mail.ru
https://doi.org/10.5281/zenodo.17318925
Relevance:
Wormwood (Artemisia (spp.) is widespread in the flora of Kazakhstan and has long
been used in folk medicine as a medicinal plant for gastrointestinal diseases, as an anti-inflammatory,
antimicrobial, and restorative. Currently, the demand for natural plant materials in the
pharmaceutical, food, and cosmetic industries is growing.
Target:
Development of a technology for obtaining dry extract and essential oil, rich in
biologically active compounds, from the plant wormwood (Artemisia spp.), determination of their
qualitative composition and substantiation of the possibility of their use in the pharmaceutical, food
and cosmetic industries.
Research methods.
Methods of raw material preparation: Collection of wormwood herb and drying in the shade at
a temperature of 40–45 °C. Grinding and fractionation (particle size 3–8 mm).
Methods for obtaining dry extract: Extraction; Maceration or percolation with 70% ethyl
alcohol. Filtration: Vacuum filter. Concentration: Rotary evaporator under vacuum (≤50 °C). Drying:
Spray or vacuum dryer.
Methods for obtaining essential oil: Hydrodistillation (Clevenger apparatus) or steam
distillation. Essential oil is isolated and dried with sodium sulfate.
Physicochemical analysis methods: Organoleptic analysis (color, odor, taste, consistency).
Determination of moisture, ash content, and yield. Determination of dry extract solubility.
Chemical analysis methods: HPLC – determination of flavonoids and lactones. GC-MS ( GC -
MS ) – determination of essential oil content.
Methods for assessing biological activity: Determination of antioxidant activity (DPPH
method). Study of antimicrobial activity (using antimicrobial test strains, agar diffusion method).
Results:
A scientifically based technology for obtaining dry extract and essential oil from the
herb of wormwood ( Artemisia) is proposed. spp .).
* Physicochemical parameters of dry extract (moisture, ash content, solubility) were determined
and standardized.
* The chemical composition was analyzed and the main biologically active substances (
flavonoids , sesquiterpene lactones, essential oil components) were characterized.
* The qualitative and quantitative composition of the essential oil was determined using gas
chromatography-mass spectrometry.
* The yield of the obtained products is calculated.
* The antioxidant and antimicrobial activity of the extract and essential oil of wormwood has
been experimentally proven.
* The production of natural products suitable for use in the pharmaceutical, food and cosmetic
industries has been substantiated.
The use of local plant materials promotes import substitution and the development of domestic
production.
75
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Conclusions:
Dry extract and essential oil of wormwood are biologically active natural
products. The developed technology allows for the effective use of local plant materials, import
substitution, and the creation of new herbal remedies.
