167
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
FORMULATION AND EVALUATION OF NOVEL DOSAGE FORMS OF
GLYCYRRHIZIC ACID
Zholayeva Zhuldyz
Bazhykova K.B.
Al-Farabi Kazakh National University, Almaty, Republic of Kazakhstan
e-mail: zholaevaa@gmail.com, tel. 87082456181
https://doi.org/10.5281/zenodo.17328731
Actuality:
Glycyrrhizinic acid, a triterpene saponin, is a biologically active compound known
for its pronounced antioxidant and anti-inflammatory properties. These pharmacological effects
highlight its potential as a valuable component in the development of ophthalmic formulations.
Despite the wide range of research on glycyrrhizinic acid, its therapeutic potential in ophthalmology
remains underexplored, which emphasizes the relevance of further studies in this direction.
Purpose of research:
To explore the potential of glycyrrhizinic acid, a triterpene saponin
derived from licorice root, through the development of innovative dosage forms.
Materials and methods:
Substance: root of the licorice -250 gr;
Reagents: 5% H2SO4, 28 ml; Acetone – 300 ml; Alcohol – 90%, 300 ml; КОН – 2 g; Glacial
acetic acid – 50 ml;
Devices: analytical scales, flasks, reverse chiller, filter paper, porcelain glass, pipette, Buchner
funnel;
Methods: Extraction method, Laboratory grinder ML 1, IR spectra, Pass online;
Result:
The biologically active compound glycyrrhizic acid was obtained by aqueous
extraction. The crude extract was further purified using acetone precipitation followed by repeated
washing with alcohol. Upon triple isolation, the yield of glycyrrhizic acid increased progressively:
1.44% in the first extraction, 1.68% in the second, and 1.88% in the third.
2. The isolated compound was characterized using IR spectroscopy and confirmed as
glycyrrhizic acid. Its properties were compared with industrial and pure reference samples,
demonstrating consistency in quality.
3. Based on the obtained substance, two ophthalmic dosage forms were developed in
accordance with pharmaceutical requirements. Six ointment prototypes were prepared, from which
formulation No. 5 (lanolin 2.5 g; vaseline 15 g) was identified as the most optimal, and a
corresponding quality specification was established. In addition, a stable and transparent eye drop
formulation was achieved, with its own quality specification prepared.
Conclusion:
Glycyrrhizic acid represents a promising biologically active compound with
diverse pharmacological effects, including anti-inflammatory, antioxidant, and antiviral activities.
The technology applied in this study allows improvement of its purity, thereby enhancing both quality
and potential economic value. Further pharmacological investigation of the developed dosage forms
may pave the way for the creation of original ophthalmic drugs aimed at treating common eye
diseases. Additionally, chemical modification of glycyrrhizic acid is recommended as a strategy to
enhance its biological activity and broaden its therapeutic applications.
