243
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
LOPHANTHUS ANISATUS (BENTH) ANALYSIS OF QUERCETIN CONTENT IN
THE AERIAL PARTS
Ibragimova D.M.
Farmanova N.T.
Ibragimova S.B.
Jumaqulova D.J.
Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan
e-mail: dildoraibragimova825@gmail.com
https://doi.org/10.5281/zenodo.17333537
Relevance:
According to the data reported in the literature, the raw material of lophanthus
anisatus (Benth.) exhibits immunomodulatory activity and contributes to faster recovery from
complications of pneumonia, bronchitis, and bronchial asthma. The aerial parts of lophanthus anisatus
(Benth.) contain flavonoids, among which quercetin occupies a prominent position. Quercetin is
classified as a vitamin P compound and is recognized as a potent antioxidant.
Research Aim:
Determination of quercetin in lophanthus anisatus (Benth.) using High-
Performance Liquid Chromatography (HPLC).
Materials and Methods:
The object of the study was the aerial parts of lophanthus anisatus
(Benth.), cultivated in 2024. The plant raw material was collected in accordance with generally
accepted pharmacognostic requirements and dried in a shaded area. To identify the flavonoids in the
plant material, High-Performance Liquid Chromatography (HPLC) was employed. The analysis was
carried out using an ‘Agilent 1260 Series’ HPLC system (Agilent Technologies, USA). The analysis
was performed on an Agilent 1200 C18 column (4.6 mm inner diameter, 150 mm length, 5 μm particle
size). The mobile phase consisted of 0.3% orthophosphoric acid (A) and methanol (B). Detection was
carried out at 370 nm, corresponding to the characteristic absorption maximum of quercetin. The flow
rate of the mobile phase was 1 mL/min, with an injection volume of 10 μL, and the column
temperature maintained at 40 °C. The total run time of the analysis was 20 minutes.
Results:
1-figure. Chromatogram of quercetin (ISN)
2-figure. Chromatogram corresponding to quercetin in Lophanthus anisatus (Benth).
As can be seen from the chromatogram presented above (Figures 1 and 2), the retention time
of the quercetin standard sample, determined using a UV detector at a wavelength of 371 nm, was
11.623 minutes.
244
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Conclusions:
The aerial parts of lophanthus anisatus (Benth.) exhibited a characteristic
chromatographic peak for quercetin at a wavelength of 371 nm with a retention time of 11.472
minutes, which corresponded to that of the working standard solution. This finding confirms the
presence of quercetin in the plant raw material. The quantitative determination revealed that the
quercetin content amounted to 19.8 mg/g.
