251
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
DETERMINATION OF THE AUTHENTICITY OF THE GLABRUS DRY EXTRACT
Karayeva N.Y.¹
Turdiyeva Z.V.¹
Abdullajonova N.G‘.²
¹ Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan
²Institute of Bioorganic Chemistry named after
A.S. Sodiqov, Tashkent city, Republic of
Uzbekistan
e-mail: k.nargiz2107@gmail.com
https://doi.org/10.5281/zenodo.17333630
Relevance:
according to literature data, about 250 species of the plant
Rhus
belonging to the
family
Anacardiaceae
are known and occur on almost every continent. Both the world scientific
community and researchers in our country are studying the composition of
Rhus
species and exploring
their practical applications. The smooth sumac (
Rhus glabra
), a member of this family, was used in
folk medicine by Avicenna (Abu Ali ibn Sino). Preparations made from this plant were applied in the
treatment of intestinal ulcers, ear pain, facial nerve paralysis, to enhance the function of the digestive
organs, for diarrhea, to stop bleeding, for diabetes, and to treat various other ailments.
Purpose of the study:
the main objective of our research was to determine the authenticity of
the conditionally named “Glabrus” dry extract obtained from the smooth sumac (
Rhus glabra
) plant
using the thin-layer chromatography (TLC) method.
Materials and Methods:
the study was carried out in accordance with Section 2.2.27 (TLC
method) of the State Pharmacopoeia of the Republic of Uzbekistan, Vol. I, Part 1.
First, 100 mg of the working standard sample was dissolved in 10 ml of a methanol–water (70:30)
mixture and filtered. The filtrate was used as the standard solution. A color-developing reagent was
prepared by mixing equal volumes of the following 0.1 % alcoholic solutions: aluminum chloride, 4-
aminoantipyrine, antimony (III) chloride, and diphenylamine. This reagent was used for spraying the
plates.
For the test solution, 1.0 g of the powdered sample was dissolved in 10 ml of a methanol–water
(70:30) mixture and filtered. Depending on the active components of the plant, different mobile-phase
compositions were selected to separate various active substances.
Procedure:
the TLC analysis was performed in three stages:
Application:
A small amount of
the prepared solution was applied to one edge of a TLC plate (coated with a thin layer of silica gel)
using a micropipette. After application, the solvent quickly evaporated, leaving small spots on the
silica gel.
Development:
The spotted plate was carefully placed in a chamber containing the mobile
phase. The chamber was closed, and the solvent front was allowed to rise evenly.
Identification:
After the colored spots separated, they were immediately visible. Because many substances are
colorless, the plate was treated with the fluorescent reagent and examined under UV light.
The retention factor (Rf) was calculated as: Rf = distance traveled by the substance
distance traveled by the solvent front Rf=\frac{\text{distance traveled by the substance} }{\text
{distance
traveled
by
the
solvent
front}}Rf
=distance
traveled
by the
solvent
frontdistance traveled by the substance. The Rf value always lies between 0 and 1 and remains
constant only if chromatographic conditions—such as the solvent system, type and thickness of
adsorbent, sample volume, and temperature—are unchanged.
Results:
the experimental results showed that the spot of the test solution corresponded to that
of the standard solution, with an Rf value of 0.6.
252
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Conclusions: b
ased on the results obtained, the authenticity of the Glabrus dry extract, when
examined by thin-layer chromatography, meets the requirements of the State Pharmacopoeia.
