257
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
DETERMINATION OF THE MONOSACCHARIDE COMPOSITION BY PAPER
CHROMATOGRAPHY METHOD FROM ACORUS CALAMUS L., HELICHRYSI
MARACANDICUM POP EX KIRP. AND AERVA LANATA L.
Mahmudova.L.A.
Khusainova.R.A.
Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan
e-mail: makhmudova.l.a.1610@gmail.com
https://doi.org/10.5281/zenodo.17333711
Relevance:
Plant polysaccharides are multifunctional biopolymers with a wide range of
pharmacological activities. Water-soluble polysaccharides (WSP), pectin substances (PS), and
hemicelluloses (HC) deserve special attention because of their antioxidant, immunomodulatory, anti-
inflammatory, and wound-healing properties. The plants Acorus calamus L., Helichrysi
maracandicum Pop ex Kirp. and Aerva lanata L. are widely used in folk and traditional medicine, but
their combined composition has not been studied for the first time.
The aim of this study
was to determine the monosaccharide composition of the extracts
obtained from the following plants: Acorus calamus L., Helichrysi maracandicum Pop ex Kirp., and
Aerva lanata L. using paper chromatography.
Materials and methods:
Different polysaccharide groups were separated using the above-
described method. Chromatographic analysis revealed that the alcohol-soluble sugars consisted of
fructose, glucose, and sucrose. Water-soluble polysaccharides were extracted using water extraction.
First, water-soluble polysaccharides (WSP) were isolated, followed by the sequential isolation of
pectin substances (PS) and hemicelluloses (HMC). Before analysis, the raw materials were cleaned
of foreign substances. One hundred grams of crushed raw materials (Acorus calamus L. (root),
Helichrysi maracandicum Pop ex Kirp. A. lanata L.(flowers) and Aerva lanata L. (above-ground
parts), and three test mixtures were treated twice with a hot mixture of methanol and chloroform (1:1)
to remove dyes and non-carbohydrate components. The raw materials were then separated by
filtration and dried afterward. The isolated substances were analyzed using paper chromatography
(PC) Filtrac-FN 13, 18 (Germany). The following solvents were used in the following ratio: n-
butanol-pyridine-water (6:4:3). The following reagents were used for opening: 1) acid phthalate
aniline (5 min, at 100°C), and 2) 5% urea solution.
Results:
The dried raw material was extracted twice with hot ethyl alcohol (1:6) at 82 °C for 1
h. The alcohol extracts were combined, evaporated, and analyzed chromatographically in system 1,
resulting in the detection of traces of glucose, sucrose, and fructose. After complete acid hydrolysis
of WSP, PS, and HMC, 100 mg of the isolated polysaccharides were hydrolyzed with 3 ml of 1n
H
2
SO
4
solution at 100°C: WSP for 8 h and PS and HMC for 24 h. After the specified time, the
hydrolysate was placed in a beaker and neutralized with barium carbonate. The resulting precipitate
was filtered, and the filtrate was deionized with KU-2 cationite, evaporated to a small volume (0.5
ml), and chromatographed on FN-18 paper in an n-butanol-pyridine-water (6:4:3) system with
specific monosaccharides (control samples). After drying, the chromatograms were treated with acid
aniline phthalate and heated in an oven at 110°C for 1-2 minutes. Galactose, arabinose, and glucose
were found in the monosaccharide composition of the polysaccharides.
Conclusion:
The monosaccharide composition of polysaccharides in the extracts obtained from
the collection of plants Acorus calamus L., Helichrysi maracandicum Pop ex Kirp., and Aerva lanata
L. was studied using paper chromatography. Given that their composition is very similar to that of
258
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
the plants studied separately, as well as the preservation of high quantitative productivity, these plant
collections can be used as effective biological products in the future.
