324
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
IN VITRO CULTIVATION OF RED-SOIL ASTRAGALUS (ASTRAGALUS TERRAE
RUBRAE BUTKOV)
Khoshimova N.Sh.
Ubaydullayeva X.A.
Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan
e-mail: khoshimova07@gmail.com
https://doi.org/10.5281/zenodo.17336931
Relevance:
The flora of Uzbekistan is rich and diverse, containing numerous rare and endemic
plant species. Among them, Red-soil Astragalus (Astragalus terrae rubrae Butkov) is a unique
endemic species listed in the “Red Book of Uzbekistan.” This plant is distinguished by its high content
of bioactive compounds such as flavonoids, saponins, polysaccharides, and organic acids. These
components exhibit cardioprotective, immunostimulatory, and anti-inflammatory activities, making
the species a valuable resource for pharmaceutical applications. However, the progressive reduction
of its natural populations highlights the necessity of employing in vitro biotechnological approaches
for its conservation and large-scale propagation.
Aim of the study:
To develop effective in vitro propagation methods for Red-soil Astragalus,
to investigate the influence of phytohormones on regeneration, and to evaluate the pharmaceutical
potential of the obtained plant material.
Materials and Methods:
Sterilization: Seeds and vegetative explants were surface-sterilized
with ethanol and sodium hypochlorite solutions prior to inoculation. Culture medium: MS medium
was supplemented with various concentrations of BAP, kinetin, IAA, and NAA to assess regeneration
efficiency. Callus induction and regeneration: The role of cytokinin-auxin balance in organogenesis
was evaluated. Pharmaceutical analysis: Regenerated plants were screened for flavonoids and
saponins using qualitative pharmacognostic assays.
Results:
The combination of BAP (1.0 – 2.0 mg/L) + IAA (0.5 mg/L) was the most effective
for shoot regeneration, while NAA (0.5 – 1.0 mg/L) promoted root induction. The regenerated plant
material demonstrated the presence of flavonoids and saponins, confirming its pharmaceutical
significance.
Conclusions:
The in vitro propagation of Astragalus terrae rubrae Butkov not only contributes
to the conservation of this endemic species but also provides a sustainable source of valuable raw
material for the pharmaceutical industry. Due to its richness in bioactive compounds, the plant
represents a promising candidate for the development of cardioprotective, immunomodulatory, and
antioxidant drugs.
