327
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
LICORICE ROOT (GLYCYRRHIZA GLABRA L.) AS A MEDICINAL PLANT FROM
SOUTHERN KAZAKHSTAN
Murzabaev B.A.
Head of the Research Laboratory "Agro-Industrial Complex Engineering",
Shymkent city, Republic of Kazakhstan
https://doi.org/10.5281/zenodo.17336967
Relevance:
Licorice (Glycyrrhiza spp.) is a genus of perennial legumes whose roots are widely
used in traditional medicine as an expectorant, anti-inflammatory, and antiviral agent. In Kazakhstan,
licorice is found in the steppes and along moist river floodplains, especially in the southern regions
(Turkestan, Zhambyl, and Almaty regions). In recent years, there has been intensive harvesting of
wild-growing stocks, which puts the species at risk in some areas. Objective: To characterize the
phytochemical profile of licorice roots collected in the southern regions of Kazakhstan and to assess
the potential of licorice for the phytomelioration of saline soils.
Materials and Methods:
Raw material collection. Locations: three sites in southern
Kazakhstan (roughly: Region A - river floodplain; Region B - steppe area; Region C - salt marshes
around oases). Time: late autumn (before root dormancy) to maximize the accumulation of secondary
metabolites.
Preparation:
root washing, drying at 40–45°C to constant weight, grinding to powder.
Extraction. Solvents: 70% methanol (polyphenol profile), water (traditional), chloroform/ethyl
acetate (lipophilic fractions). Method: maceration for 24–48 hours at room temperature with a 1:10
ratio (raw material:solvent), filtration, and solvent evaporation under vacuum. Phytochemical
analysis. Total phenolic activity: Folin-Ceccalteu method (TPC), results expressed as mg gallic acid
equivalent per g of raw material (mg GAE/g).
Flavonoids:
aluminum chloride complexation method. Field phytomelioration model.
Results:
The 70% methanol extract is characterized by the highest content of phenols and
flavonoids.
Glycyrrhizin is the main quantified component (≈34 mg/g dry root), confirming the
pharmacological relevance of the raw material (anti-inflammatory, sweet taste).
Phytomelioration. Field experiment on saline plots: plots (control and licorice sowing),
measurement of soil electrical conductivity (EC), soluble salt content, and organic matter before and
after 2–3 seasons.
When licorice is planted in slightly saline soils, a tendency toward decreased overall salinity
and increased organic matter in the rooting horizon is observed within 2–3 years (depending on
climate and initial soil conditions).
Literature data confirm that Glycyrrhiza glabra possesses antioxidant, anti-inflammatory, and
antiviral activity; the main differences between populations are the quantitative ratios of metabolites,
making local studies important for the standardization of raw materials.
For southern Kazakhstan, issues of sustainable harvesting and protection are important, as
intensive illegal root harvesting has been recorded in a number of areas.
Conclusions:
Licorice root collected in southern Kazakhstan is a promising raw material for
the pharmaceutical and cosmetic industries. The conducted and proposed experiments provide a
methodological basis for assessing the quality of the raw material, its pharmacological potential, and
its environmental application in phytomelioration.
