330
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
SYNTHESIS OF POLYMERIC NITROGEN DONATIONS BASED ON INULIN
DERIVATIVES
1
Abduhomidova F.O.
2
Khusenov A.Sh.
2
Rakhmanberdiev G.
2
Ibragimova K.S.
1
Karshi State Technical University, Karshi city, Republic of Uzbekistan
2
Tashkent Institute of Chemical Technology, Tashkent city, Republic of Uzbekistan
https://doi.org/10.5281/zenodo.17337056
Relevance:
Nitric oxide (NO) donors are widely used in the treatment of cardiovascular
diseases, pulmonary hypertension, and are also actively studied in the treatment of neurodegenerative
pathologies. Such drugs as Nitroglycerin, Molsidomine and Nicorandil are capable of releasing nitric
oxide. In the context of the growing number of chronic diseases and an aging population, there is a
growing need for effective and safe means regulating the bioavailability of NO. In addition, the
improvement of synthesis methods makes it possible to obtain nitrogen donors with a given structure
that ensures controlled release of NO under physiological conditions of the div. For these reasons,
the development of new methods for the synthesis of nitrogen donors with controlled release and high
biocompatibility is becoming a particularly interesting task.
Purpose of the study:
Synthesis of new nitrogen donors by targeted chemical modification of
inulin esters.
Materials and methods:
For synthesize nitrogen donors, sodium salt of carboxymethylinulin
(CMI) was initially synthesized by alkylation of the polysaccharide in the presence of
monochloroacetic acid. For this, inulin was dispersed in ethanol for 2 hours, then a 15% alkali solution
was added and stirred for 1 hour. The reaction was carried out with Na-MCUA (sodium salt of
monochloroacetic acid) at a molar ratio of inulin:Na-MCUA=1:4, a temperature of 70°C for 1.5
hours. Purification was carried out by extraction with 96% ethanol, then the samples were dried at
50-60°C. A KMI sample with a substitution degree of 34 mol.%, dried to a constant weight, was
esterified under homogeneous conditions with a nitrating mixture of the following composition:
HNO
3
÷H
3
PO
4
÷P
2
O
5
, wt.% = 20:75:5 at room temperature for 30 minutes. Then the nitrating mixture
was poured into a tenfold volume of ice-cold distilled water, the resulting white precipitate of inulin
nitroester was separated on a Schott glass filter and washed in distilled water until the washing waters
were neutral, dried in a vacuum oven and analyzed.
Results:
The FTIR spectra of the synthesized compounds showed absorption bands with
maxima in the region of 1650 cm
-1
(N=O), 838 cm
-1
(N-O), 1280 cm
-1
(symmetric stretching
vibrations of N=O bonds), 1745 cm
-1
(C=O). Elemental analysis showed that the weight nitrogen
content in the sample was 9.7% and its substitution degree for -NO
2
groups was 1.50 mol%. The
obtained data indicate the efficiency of nitration with preservation of the main structure of the
polysaccharide. The substitution degree indicates a partial but controlled introduction of nitro groups,
which is important for subsequent regulation of their release.
The rate of nitro group splitting at pH 7.4 was studied. Thus, it was found that nitro groups
included in the polymer chain have a prolonged release in a weakly alkaline medium. Within 3, 6, 12
and 24 hours, the rate of nitrogen oxide release is 12, 37, 60 and 85%, respectively. These data prove
that nitro groups contained in the inulin structure are unstable in a weakly alkaline medium and begin
to split off within 24 hours.
331
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Conclusions:
The obtained nitro derivatives of inulin are characterized by a controlled level of
substitution and the ability to prolonged release of nitrogen oxides in a weakly alkaline medium. This
confirms their potential as nitrogen donors with controlled release kinetics.
