336
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
SYNTHESIS AND CHARACTERIZATION OF HYDROXYAPATITE
NANOPARTICLES FOR BIOMEDICAL APPLICATIONS
J.T. Abdusalamov
1
M.K. Suyundikov
1
Sh.I. Azizov
1
M.A. Sharipov
2
A.S. Turayev
1
1
Institute of Bioorganic Chemistry, Tashkent city, Republic of Uzbekistan
2
Faculty of Mechanical Engineering, Yonsei University. Seul, Republic of Korea
e-mail: javokhirabdusalomov@gmail.com
https://doi.org/10.5281/zenodo.17337277
Purpose of the study:
In this study, the synthesis of hydroxyapatite (HA) is reported. Currently, in addition to the
traditional (oral and injection) methods of drug delivery to the div, delivery using some chemical
means (micelles, liposomes, etc.) is more preferred by researchers. When using these tools, the
advantages of such substances are that they can reduce the cytotoxicity of chemotherapeutic agents,
reduce the amount of administered drugs, target delivery, and other features. In our studies, the
nanopolymers used for drug delivery encountered some barriers in crossing the cornea of the skin.
When HA nanoparticles were used they provided mechanical strength due to their crystalline
structure. A hydrothermal synthesis method was used to synthesize HA nanoparticles.
Materials and methods:
Ca(NO
3
)
2
*2H
2
O and (NH
4
)
2
HPO
4
(Sigma Aldrich) salts and hydrothermal synthesis method
were used to synthesize HA nanoparticles. A mixture of Ca(NO
3
)
2
*2H
2
O and (NH
4
)
2
HPO
4
salts (in
a ratio of 0.4:0.1 moles, respectively) was dissolved in 20 mL of distilled water and stirred using a
magnetic stirrer for 30 minutes at room temperature. The resulting solution had a pH of 3 (0.1 M
HNO
3
was used to adjust the pH). After adding the acid, the clear solution turned into a colloidal
suspension. The total volume of the suspension was reduced to 25 mL.
The reaction was allowed to proceed for 72 hours at 60 °C. After completion of the reaction,
the resulting product was collected by centrifugation (10000 rpm, 10 minutes, 3 times). The obtained
precipitate was washed with ethyl alcohol and dried in an oven at room temperature.
Results:
In this study, HA particles were synthesized using the hydrothermal method. Our team modified
this method by changing the temperature and reagent concentration. These modifications have been
used to produce HA nanoparticles ranging in size from nanometers to micrometers. Under optimized
conditions, we were able to obtain HA nanoparticles with a hydrodynamic size distribution of
approximately 270 nm (Figure 1). The synthesized HA nanoparticles were first screened using a zeta-
sizer to measure their hydrodynamic size. The size and morphology of HA nanoparticles were
analyzed using a SEM instrument (Jeol JSM-IT200LA) at the Uzbekistan-Japan Youth Innovation
Center.
337
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Conclusions:
In conclusion, it can be said that when the carrier drug was bound to HA nanoparticles, it was
observed that the mechanical strength was improved compared to the previous state. Of course, this
strength was found to depend on the particle size of the HA nanoparticles, and further research aimed
to develop nanoparticles of higher quality using other synthesis methods.
0
500
1000
0
1
2
3
4
5
6
7
Freq
ue
nc
y
Size (nm)
Average size = 270 nm
SD = 130 nm
