340
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
ISOLATION OF THE METABOLITES FROM
ASTRAGALUS ALOPECIAS
AND
DETERMINATION OF THEIR CHEMICAL STRUCTURE
Akhmedov A.D.
1
Mamadalieva N.Z.
1, 2
1
Institute of the Chemistry of Plant Substances, Tashkent city, Republic of Uzbekistan
2
Alfraganus University, Tashkent city, Republic of Uzbekistan
e-mail: ahmedovanvar26@gmail.com Tel: (90) 010-61-50
https://doi.org/10.5281/zenodo.17337332
Relevance
: Currently, the drugs used in medicine are mainly synthetic drugs, their therapeutic
effect is effective and fast, but they have some harmful effects. Therefore, a lot of work is being done
in the world to create drugs from relatively harmless plant raw materials. In Uzbekistan, providing
our people with harmless, high-quality, affordable drugs remains one of the important tasks of our
state policy.
Purpose of the study:
Astragalus
(family Leguminosae) is a large genus widespread in
temperate regions of the world, mainly in Eurasia and North America.
Astragalus
has been used in
Central Asian traditional medicine for centuries to treat kidney and bladder stones. Phytochemical
studies of
Astragalus
species have led to the isolation and identification of triterpenoid saponins,
flavonoids, phenylpropanoids, alkaloids and some other compounds. In terms of medicinal use and
scientific research, our scientific work focuses on the isolation of biologically active substances from
the
Astragalus alopecias
Pall. species of the
Astragalus
genus.
Astragalus alopecias
Pall. - locally known as Tulkikabi astragal, is spread out in Central Asia
(Tian-Shan, Pamir-Alay, Kopetdog), Afghanistan, Iran, and Pakistan. It is a perennial plant growing
to 40-80 cm tall and flowering from May to June. Based on the results of the literature review and
examination, it was found that there is almost no available information about the chemical
components of this species. We continued our search for natural compounds in the
Astragalus
genus
by studying the aerial parts of
A. alopecias
collected from Boysuntog (Surkhandarya region,
Uzbekistan).
Methods and techniques:
Thin layer chromatography of the methanol extract of
A. alopecias
revealed the presence of several flavonoids and triterpene glycosides in the plant. Processing of the
methanol extract with ethyl acetate yielded the ethyl acetate fraction. This fraction was subjected to
multiple column chromatography to isolate the isoflavones ononine (C
22
H
22
O
9
) (1) and daidzein
(C
15
H
10
O
4
) (2). The methanol extract was treated with butanol to obtain a butanol fraction. This
fraction was subjected to multiple column chromatography to identify the glycoside cycloalpioside
D (C
35
H
58
O
9
) (3).
Results:
The chemical structure of the substances was determined by mass and NMR spectra.
This substance was previously isolated from some
Astragalus
species. It was first identified from
A.
alopecias
.
Conclusions:
For the first time, three secondary metabolites were isolated from
A. alopecias
.
Research is currently being conducted to evaluate the biological activities of the isolated compounds.
341
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
1
2
3
