353
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
ANALYSIS OF FLAVONOID COMPOSITION OF
ALLIUM MOTOR
BY HPLC
Yuldoshev S.S.
National University of Uzbekistan, Tashkent city, Republic of Uzbekistan
e-mail: yoldoshevsohibjon35@gmail.com
https://doi.org/10.5281/zenodo.17337480
Relevance:
In recent years, the isolation of bioactive compounds from natural sources and the
evaluation of their pharmaceutical potential have become important directions of scientific research.
In particular, plants of the genus
Allium
have long been used in traditional medicine. Their chemical
composition has been shown to include flavonoids, phenolic acids, vitamins, and other biologically
active compounds. In this context, a comprehensive chemical analysis of
Allium motor
and the
assessment of its pharmaceutical and bioactive properties represent a relevant task.
Aim of the study.
The main aim of this study was to identify flavonoids present in the extracts
of vegetative parts of
Allium motor
using high-performance liquid chromatography (HPLC), to
determine their quantitative proportions, and to substantiate their pharmaceutical significance.
Methods.
As research objects, ethanol extracts were prepared from the leaves and bulbs of
Allium motor
. Analytical tests were carried out using an Agilent HPLC system operating with
ChemStation software. A diode array detector (DAD) was used at wavelengths of 254 nm, 265 nm,
and 281 nm. Standard reference substances such as apigenin, rutin, and resveratrol were applied for
comparison.
Results.
HPLC analysis confirmed the presence of several flavonoid compounds in
Allium
motor
extracts. Their relative contents are presented in Table 1.
Table 1.
Relative content of flavonoids in
Allium motor
extract (based on HPLC results)
№
Compound
Retention time (min) Relative content (%)
1
Apigenin
1.71–1.73
18.3 – 24.0
2
Rabinin
1.93–1.94
4.6 – 40.9
3
Rutin
2.35
3.7 – 3.9
4
Resveratrol
7.13
3.7 – 22.3
5 Other flavonoids
1.62–1.81
16.4 – 46.8
The results indicate that
Allium motor
is rich in flavonoids. Rabinin and apigenin were
identified as the main components, while resveratrol and rutin also made up a significant proportion
of the composition. The diversity of flavonoids demonstrates the strong antioxidant potential of the
plant. Furthermore, the high content of rabinin suggests possible immunomodulatory and anti-
inflammatory properties. The considerable presence of apigenin supports its sedative and antiallergic
activity. The detection of resveratrol indicates potential cardioprotective and anticancer effects.
Compared to data on other
Allium
species reported in the literature,
Allium motor
appears to be
distinguished by its richness in bioactive compounds. Thus, extracts of this plant may represent a
promising source for the development of new phytopharmaceuticals.
Conclusions. Allium motor
is rich in flavonoids, with rabinin, apigenin, rutin, and resveratrol
being the main components.
The chemical composition of the plant confirms its antioxidant and
pharmaceutical potential.
The findings provide a scientific basis for the development of new
phytopreparations and their use in clinical pharmacy.
