372
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
DETERMINATION OF ANTIOXIDANT PROPERTIES OF FACTORY-GROWN
CHICKEN EGGSHELLS
Fayziyev X.O.
Uz. Res. Military officer of the Ministry of Defense, researcher of chemical sciences.
Islomov Akmal Xushvaqovich
Doctor of Chemical Sciences, Institute of Bioorganic Chemistry, Academy of Sciences of the
Republic of Uzbekistan.
Mustafakulov M.A.
Senior Researcher, Institute of Biophysics and Biochemistry under the M. Ulugbek National
University of Uzbekistan., PhD
https://doi.org/10.5281/zenodo.17337909
Relevance of the study:
Today, bone diseases are increasing due to calcium deficiency, and to
prevent and eliminate this condition, eggshells are a natural source rich in calcium. They are not only
a source of calcium, but also play an important role in the absorption of D3, which is obtained with
solar energy, into the div.
Purpose of the study:
To determine the antioxidant properties of factory-grown chicken
eggshells.
Research methods:
Washed and cleaned eggshells, dried at room temperature, were ground
into powder. The antioxidant activity of the powdered sample was determined by the method of in
vitro autooxidation of adrenaline.
Research results:
The antioxidant activity of eggshells was determined by the inhibition of the
in vitro autooxidation reaction of adrenaline.
For this purpose, 2.0 ml of 0.2 M sodium carbonate (Na2CO3-NaHCO3) buffer with pH=10.65,
56 μl of 0.18% adrenaline (epinephrine) hydrochloride solution were taken, 30 μl of antioxidant
preparation were added and, with rapid mixing, the sample was examined in a 10 mm cuvette at a
wavelength of 347 nm for 30 seconds to 10 minutes on a Cary 60 UV-Vis Agilet Technologies
spectrophotometer. For this purpose, 2.0 ml of 0.2 M sodium carbonate (Na2CO3-NaHCO3) buffer
with pH=10.65, 56 μl of 0.18% adrenaline (epinephrine) hydrochloride solution were taken, 30 μl of
antioxidant preparation were added and, with rapid mixing, the sample was examined in a 10 mm
cuvette at a wavelength of 347 nm for 30 seconds to 10 minutes on a Cary 60 UV-Vis Agilet
Technologies spectrophotometer. The amount of the test substance (concentration of the extract in 1
ml is 1 mg) is used as a standard. As a control sample, 0.2 M 2.0 ml buffer, 0.18% 56 μl (5.46 mM)
adrenaline is used.
The antioxidant activity was expressed as a percentage of the inhibition of adrenaline
autooxidation and was calculated by the following formula.
АА%=
D1−D2 x100
𝐷1
Optical density of adrenaline hydrochloride solution added to buffer D1;
Optical density of the extract under study and adrenaline hydrochloride added to buffer D2.
Statistics were tested using the T-student test and Orijen 6.1 US software.
The antioxidant capacity of the drugs is determined for the in vitro form.
Sample
Composition
Solubility
In vitro; mkg/ml
Home eggshell extract
Eggshell extract
30% alcohol and water
30
373
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Conclusions:
The antioxidant activity of the preparations was determined by the method of in
vitro autoxidation of adrenaline. The antioxidant activity of the studied preparations was assessed by
chemical tests. The antioxidant activity of the preparations was determined by the inhibition of the in
vitro autoxidation reaction of adrenaline and the prevention of the formation of free oxygen species.
The preparations were compared with the standard antioxidants quercetin and gliclazide. The
obtained preparations show the presence of antioxidant properties.
Quercetin
Japanese
Embassy
30% alcohol and water
30
Gliklazide
-
water
30
