415
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
DETERMINATION OF BIOLOGICALLY ACTIVE SUBSTANCES AND
MICROBIOLOGICAL PURITY IN THE NEW CHOLESTERIC COLLECTION
H.K. Olimov
A.A. Ismoilov
Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan
e-mail: gulnozasadikova1@mail.ru, tel. +998946451962
https://doi.org/10.5281/zenodo.17341119
Introduction
Philogenetic agents, normalizing bile flow, are effectively used in the treatment
of liver and gallbladder pathologies. For the treatment and elimination of bile stasis, along with
preparations of synthetic origin, medicinal plants with a pronounced choleretic effect are often used.
Immortelle flower phytopreparations enhance the liver's functional capacity, thereby increasing bile
secretion, leading to an increase in the amount of cholates in bile and a decrease in bile viscosity;
corn stalks are effective for gallbladder and urinary tract infections, as well as liver diseases and
jaundice.
Purpose of the study
Determination of biologically active substances and microbiological
purity of the new bile collection.
Materials and methods A choleretic collection consisting of Cynara scolymus (L.), Asteraceae
(Asteraceae) family's thorny artichoke leaves; and
Helichrysum arenarium
(L.) Moench, Asteraceae
family
- Asteraceae;
corn stalks with stamens
Zea mays
(L), Beetles family
- Poaceae,
used as a
medicinal product.
Composition:
Thorn artichoke leaves
30%
Sandflower immortelle
35%
Corn stalks with stomata
35%
Numerical indicators were determined by pharmacopoeial methods.
Qualitative reactions were determined using pharmacopoeial methods.
1 г
Experimental part
Add 30 ml of 50% ethyl alcohol to the ground raw material and boil in
a water bath for 30 minutes. After cooling, the contents of the flasks are filtered through cotton. The
resulting extract is applied using a capillary to the start line of the chromatographic paper (15x15 cm).
The chromatographic paper with the applied sample is air-dried, placed in a chamber with 2% acetic
acid, and chromatographed using the ascending method. When the solvent front reaches the edge of
the chromatographic paper, it is removed and dried in a fume hood for 2 minutes. When the
chromatographic paper is viewed in UV light, blue fluorescence (hydroxycoricic acid) appears.
To 2 ml of the test solution, 2 ml of aluminum chloride 10% solution in 96% alcohol is added,
a greenish-yellow coloring is observed (flavonoids)
Determination of the microbiological purity of the new bile collection was carried out in the
microbiology laboratory of the Medical Products Testing Center under the "Dori vositalarini
standartlashtirish Ilmiy Markazi" LLC according to the methodology of the State Pharmacopoeia of
the Republic of Uzbekistan. The tests were conducted under sterile box conditions, at room
temperature of 21°C and humidity of 68%.
1 гThe "collection" allowed the presence of no more than 10
5
total number of aerobic bacteria
and no more than 10
4
total number of fungi and no more than 10
3
enterobacteria and some other
gram-negative bacteria, as well as the absence of Escherichia coli, the absence of Salmonella.
416
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Conclusions
In the new bile collection
o
biologically active substances such as oxidoric acid
and flavonoids have been identified. The microbiological purity of the collection corresponding to
the State Pharmacopoeia of the Republic of Uzbekistan was determined.
