451
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
RESEARCH ON THE PHARMACOLOGICAL ACTIVITY OF ISATIS TINCTORIA
SEED OIL
Khakimjanova Sh.O.
Tillaeva G.U.
Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan
https://doi.org/10.5281/zenodo.17342266
Relevance.
I. tinctoria
seed oil contains a number of bioactive components, including alkaloids,
flavonoids, and phenolic compounds, which may have anti-inflammatory, antioxidant, antimicrobial,
and antiviral effects. Studies of the pharmacological activity of the oil may provide new data for its
use as a natural remedy for various diseases, such as respiratory tract infections, skin inflammations,
wounds and burns.
Aim of the study:
to study the wound-healing activity of “
I. tinctoria
seed oil” in the scientific
laboratory of innovative pharmaceutical compounds at the Tashkent Pharmaceutical Institute.
Materials and methods:
Wound healing activity was studied using a model of complete skin
excision in mice. Experiments were conducted on 15 white inbred mice weighing 18–22 g, divided
into three groups of 5 animals.
To simulate a wound defect in animals, a 1 cm diameter area of skin was excised from the
dorsal region of the back under aseptic conditions. The wounds were not covered with bandages and
remained open throughout the experiment.
The animals received the test samples for 16 days at a dose of 500 mg/kg in the form of 100%
oil:
1. Control group – animals were administered sunflower oil at a dose of 0.01 ml/20 g of div
weight.
2. Test group – animals received “
I. tinctoria
seed oil” (TashFarmi, Uzbekistan) at a dose of
500 mg/kg, in a volume of 0.01 ml/20 g.
3. The comparative group – animals received sea buckthorn oil at a dose of 500 mg/kg, in a
volume of 0.01 ml/20 g.
Wound healing activity was assessed every other day throughout the experiment by measuring
the wound area using the
ImageJ
programme.
The criterion for effectiveness was a reduction in wound area compared to the control group.
The results were processed using ANOVA variation statistics at a significance level of p=0.05,
using GraphPad Prism version 8.0.0 for Windows, GraphPad Software, San Diego, California, USA.
Research results:
In the control group, wounds healed more slowly compared to the
experimental groups. On the 6th day, the wound area was 87.63%, and by the 16th day it had
decreased to 7.38%. Complete wound closure was not achieved by the 16th day.
In the group receiving “
I. tinctoria
seed oil”, wound healing was significantly faster. Already
on the 6th day, the wound area decreased to 50.38%, and by the 12th day it was only 6.94%. Complete
wound closure was observed on the 14th day, which indicates the pronounced wound-healing activity
of this oil.
The group receiving sea buckthorn oil also showed a high rate of wound healing. On the 6th
day, the wound area was 59.20%, and by the 10th day, it was 14.97%. Complete wound closure
occurred by the 16th day.
452
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Conclusion:
The study showed that “
I. tinctoria
seed oil” has pronounced wound-healing
activity, promoting complete wound closure by the 14th day. Sea buckthorn oil also accelerated
healing, but the process was less intense, and complete closure of wounds was observed only by the
16th day. The results confirm the promise of madder seed oil as an effective wound-healing agent.
