464
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE
“
MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS
”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
COMPARATIVE EVALUATION OF THE EFFECTIVENESS OF EMHPS AND ITS
METABOLITE EMSP ON RESPIRATORY PARAMETERS, MOTOR ACTIVITY
AND GENE EXPRESSION OF MITOCHONDRIAL COMPLEXES IN MICE UNDER
CONDITIONS OF INTERMITTENT HYPOXIA
Plisko G.A.
Ivkin D.Yu.
Saint Petersburg Chemical-Pharmaceutical University, Sankt Petersburg, Russia
e-mail: grigoriyplisko@gmail.com
https://doi.org/10.5281/zenodo.17342411
Relevance:
Intermittent hypoxia (IH) is a pathological factor that provokes oxidative stress,
which can initiate a cascade of pathological conditions, mitochondrial dysfunction, inflammation,
endothelial dysfunction and metabolic disorders. In this regard, the study of active antioxidants is
relevant. In this work, we studied the antihypoxic properties of the metabolite of
ethylmethylhydroxypyridine succinate (EMHPS) - ethylmethylsulfapyridine (EMSP) on the IH
model in mice. In previous studies, EMSP exhibited antihypoxic and antioxidant properties [1-3].
The aim of the study:
A comparative study of the effectiveness of EMSP with the native
molecule in the IH model in mice.
Materials and methods:
The study was conducted on 18 male white mice. The study was
divided into 3 groups: Group 1 – control (n=6), IG + saline; Group 2 – (n=6), IG + EMSP at a dose
of (85 mg/kg); Group 3 – (n=6), IG + EMHPS (100 mg/kg). The administration was performed
intraperitoneally for 14 days in parallel with the modeling of IG, 30 minutes before IG. Modeling of
IG was performed by placing the animals in a membrane hypoxicator. The following regime was set:
oxygen content - 6%, duration of the hypoxic cycle - 6 hours. Duration - 14 days, daily. At the
beginning and end of the study, the dynamic load was assessed (grip strength test), respiratory
parameters were measured (in a plethysmograph), behavioral indicators (in the open field (OF) and
elevated plus maze (EPM) tests, heart rate (HR) and saturation (SpO
2
) were measured. After
euthanasia, the expression of mitochondrial complex genes in the liver was determined in animals of
all groups using the polymerase chain reaction (PCR) in real time. Были выбраны такие гены как:
GAPDH – reference gene; NDUFS1 – encodes the key subunit of the complex I (NADH-
dehydrogenase); SDHA – catalytic subunit of the complex II (succinate dehydrogenase); UQCRC2
– structural component of the complex III (cytochrome bc1); COX1 – subunit of the complex IV
(cytochrome c-oxidase).
Results:
The results of the study showed that EMSP showed comparable effectiveness in terms
of grip strength, plethysmograph, SpO
2
heart rate, and in the OF and EPM tests compared to EMHPS.
According to the PCR results, a clear tendency towards expression of the UQCRC2 gene was noted
in the EMSP and EMHPS groups. Moreover, in the EMSP group, the expression was more
pronounced.
Conclusions:
The studied compound EMSP showed protective properties against the
background of IG comparable to EMHPS, and a more pronounced tendency for expression of the
UQCRC2 gene.
