Volume 03 Issue 06-2023
62
International Journal of Medical Sciences And Clinical Research
(ISSN
–
2771-2265)
VOLUME
03
ISSUE
06
P
AGES
:
62-64
SJIF
I
MPACT
FACTOR
(2021:
5.
694
)
(2022:
5.
893
)
(2023:
6.
184
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
ABSTRACT
The removal of pancreatic juice from the duodenum increases the secretion of the latter, and the reverse introduction
of juice slows it down. The mechanism of this effect has been the subject of lively discussions for many years.
KEYWORDS
Theoretical, hydrochloric acid , digestive glands.
INTRODUCTION
The study of this issue has not only theoretical, but also
practical significance, including for improving the
methods of functional diagnostics and substitution
enzyme therapy. So, based on the accumulated data, it
can be assumed that with continuous aspiration of
pancreatic juice from the duodenum by a probe, the
hypersecretory reaction of the gland is manifested if
the secretion is caused by the introduction of a
stimulant (for example, hydrochloric acid), and is
absent if it is caused by parenteral administration of
secretin with pancreasimine. Most researchers
associate the effect of inhibition of secretion with the
action of trypsinogen or its hexapeptite fragment on
the endocrine apparatus of the duodenum. At the
same time, oral administration of amylase has been
shown to reduce the amylolytic activity of rat
pancreatic homegenate. The purpose of the study:
Based on the general principle of regulation of the
enzymatic activity of the digestive glands, an
assumption was made about the differentiation of its
inhibition depending on the fermetative activity of the
duodenal contents. We conducted a study to find out
Research Article
STUDY OF THE COUNTER-EFFECTS OF THE RELEASE OF PANCREATIC
ENZYMES WITH THE INTRODUCTION OF DIGESTIVE FARMS
Submission Date:
June 11, 2023,
Accepted Date:
June 16, 2023,
Published Date:
June 21, 2023
Crossref doi:
https://doi.org/10.37547/ijmscr/Volume03Issue06-12
Sotvoldiyev B.M.
Andijan State Medican Institute, Uzbekistan
Journal
Website:
https://theusajournals.
com/index.php/ijmscr
Copyright:
Original
content from this work
may be used under the
terms of the creative
commons
attributes
4.0 licence.
Volume 03 Issue 06-2023
63
International Journal of Medical Sciences And Clinical Research
(ISSN
–
2771-2265)
VOLUME
03
ISSUE
06
P
AGES
:
62-64
SJIF
I
MPACT
FACTOR
(2021:
5.
694
)
(2022:
5.
893
)
(2023:
6.
184
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
the effect of increased amylolytic activity of duodenal
contents on pancreatic secretion. The experiments
were performed on 5 mongrel dogs weighing 12-15 kg.
Anesthesia (droperidol, aminazine, hexanal) was
performed
under
controlled
Breathing
(DP-8
apparatus). To collect pancreatic juice, the main
pancreatic duct was cannulated, a ligature B was
applied to the small duct at the site of the pyloric pulp,
B of the initial duodenum was strengthened with a
pouch suture to introduce a stimulator of the secretion
part. Blood and urine were collected through catheters
inserted into both ureters and the femoral vein. To
stimulate the secretion, 8-10 ml of 0.1 n hydrochloric
acid solution was administered intraduodenally for 1-4,
then 8-10 ml of hydrolysin acidified to pH 2 every 15
minutes for 8 hours. After 2 hours, 0.2 mg% ratvor (10
ml / h) of the enzyme preparation amylase (produced
by the Olainen plant) was injected into the duodenum
at the rate of 20 mg / h (2.5 every 15 minutes), then 2
hours of barley malt diastase hydrolysin, 1 hour
amylase and another 2 hours of hydrolysin. In the
collected juice, the content of bicorbonates (reverse
titration), protein (according to Lowry), amylase
(according to Smith-Roe modified by A.M. Ugolev),
lipase (according to Titz) and total proteolytic activity
(according to Kunitz) were determined. The volume of
urine was taken into account, the amount of amylase
was determined every hour in the urine and blood
plasma. The introduction of acidified hydrolysin into
the duodenum caused a fairly stable secretion from
hour to hour with a high content of enzymes in the
juice, slightly decreasing in volume by 6-8 hours of
experience. Intraduodenal administration of malt
diastase did not change the volume of secretion and
excretion of bicorbonates, lipase protein and
proteases in the juice. A decrease in the volume of
secretion and the release of bicorbonates due to this
was noted at the end of the experiment, apparently
due to its duration. Consequently, the activity of even
duodenal contents, a small increase in amylotic is
caused by a heterogeneous (plant) enzyme, always
leads to inhibition of pancreatic secretion of amylase.
This allows us to conclude that the inhibition of
pancreatic secretion is differentiated depending on the
type of enzymatic activity increased in the duodenum.
This implies the possibility of differentiated correction
of pancreatic enzyme excretion by: a) oral
administration of enzyme preparations with different
activity of their ingredients or preparations with the
same activity (according to the results of our study,
amylotic activity); b) inhibition of pancreatic amylase
secretion. The question of the mechanism of such
braking is complicated. It is believed that inhibition of
pancreatic secretion caused by intraduodenal
administration of trypsinogen is provided by inhibition
of the release of cholecystokinin-pancreosimin from
endocrine 1-cells of the duodenum. The excretion of
amylase in urine increased during the experiment due
to increased urine diuresis and amylotic activity,
Volume 03 Issue 06-2023
64
International Journal of Medical Sciences And Clinical Research
(ISSN
–
2771-2265)
VOLUME
03
ISSUE
06
P
AGES
:
62-64
SJIF
I
MPACT
FACTOR
(2021:
5.
694
)
(2022:
5.
893
)
(2023:
6.
184
)
OCLC
–
1121105677
Publisher:
Oscar Publishing Services
Servi
gradually reaching colossal values (50-70 times more
than in the first 2 hours of the experiment). This could
not be exogenous amylase absorbed from the
intestine, since only 2,200 units were injected into the
intestine during 2 infusions. amylase, and more than
10,000 units were excreted in the urine in 6 hours, not
to mention the fact that the absorption of amylase in
the intestine is very insignificant. It can be assumed
that under conditions of increased amylolytic activity
of the duodenal contents, a redistribution of the
exosecreted and incretireable enzyme occurs.
However, the comparison of the amount of amylase
under-excreted with juice (327232 units) as a result of
the inhibitory effect of diastase on secretion and
excreted in urine (10303 units) showed that about 3%
of the amount of amylase by which its secretion by the
gland decreased was released by the renal route, Most
likely, the increase in excretion of amylase in urine is
not associated with the effect of the injected diastase,
and is the result of a gradual increase in the
permeability of the histohematic barrier and a slightly
increased deviation of amylase from the gland into the
blood under conditions of stimulation of the gland by
hydrolysin. The very effect of inhibition of amylase
exosecretion under the action of malt diastase injected
into the duodenum is realized at the level of moderate
selective inhibition of amylase synthesis and extrusion
by the pancreas.
REFERENCES
1.
Korotko G.F - V sb: nodal issues of modern
physiology. Tomsk, 1984 pp. 139-145.
2.
Korotko G.F. Enzymes of digestive glands in the
blood (an essay on enzyme homeostasis) -
Tashkent: Medicine, 1983.
3.
Korotko G. F. - Physiol. journal., 1979, vol. 65, No. 7,
pp. 1017-1023.
