73
International Journal of Medical Sciences And Clinical Research
https://theusajournals.com/index.php/ijmscr
VOLUME
Vol.05 Issue02 2025
PAGE NO.
73-77
DOI
10.37547/ijmscr/Volume05Issue02-13
Study of sensitivity to systemic antibiotics s. Epidermidis
and s. Aureus in patients with moderate and severe acne
Zakirov Shakirbek Yusupovich
Candidate of Medical Sciences, Associate Professor of the Department of Microbiology, Virology and Immunology, Urgench Branch of
the Tashkent Medical Academy, Urgench, Uzbekistan
Babazhanov Khudainazar Radzhabovich
PhD, Senior Lecturer at the Department of Internal Diseases and Dermatovenerology of the Urgench Branch of the Tashkent Medical
Academy, Urgench, Uzbekistan
Kalandarov Oybek Odylbekovich
Assistant of the Department of Internal Diseases and Dermatovenerology of the Urgench branch of the Tashkent Medical Academy,
Urgench, Uzbekistan
Kalandarova Matluba Khozhaniezovna
Assistant Professor at the Department of Microbiology, Virology and Immunology of the Urgench Branch of the Tashkent Medical
Academy, Urgench, Uzbekistan
Zokirova Mokhira Shokirbekovna
Is a fourth–year student of the medical faculty of the Urgench branch of the Tashkent Medical Academy, Urgench, Uzbekistan
Received:
24 December 2024;
Accepted:
26 January 2025;
Published:
28 February 2025
Abstract:
The sensitivity of S. epidermidis and S. aureus isolated from patients with moderate and severe acne to
minocycline, doxylan, tetracycline and erythromycin in the treatment of this pathology. The antibiotic sensitivity
of isolated strains was determined by the disco diffusion method on Mueller-Hinton agar in accordance with the
guidelines. The data obtained make it possible to recommend the drugs minocycline and doxylan for clinical use
in the treatment of patients with moderate to severe acne in dermatological practice.
Keywords:
Acne, staphylococci, antibiotic resistance, antibacterial drugs.
Introduction:
Antibiotics (AB) are used to treat
bacterial and fungal infections (they do not affect
viruses). AB successfully fight severe bacterial
infections that the human immune system cannot cope
with on its own. They can kill bacteria or stop their
reproduction, allowing natural defense mechanisms to
eliminate them. Antibiotic resistance (AR) is the
resistance of bacteria to an antibiotic, i.e. the antibiotic
becomes familiar, habitual for the bacteria and
ineffective for treatment. The causes of ABR can be -
widespread use of antibacterial drugs (ABP), taking AB
without indications, violation of AB intake regimens, AB
content in food products, etc. Only with a full course (!)
of antibiotic therapy (ABT) does the bacteria die. If the
course is interrupted, then during this time the bacteria
only weaken under the influence of AB, but do not die.
Also, incomplete courses of ABT are one of the reasons
for the development of bacterial resistance to AB.
Microbiome resistance (MR) to antimicrobials and
antibiotics is recognized as a serious public health
problem worldwide [4]. When prescribing antibiotics, it
is necessary to consider the entire spectrum of their
antimicrobial action, since P. acnes is not the only
microorganism responsible for the development of
International Journal of Medical Sciences And Clinical Research (ISSN: 2771-2265)
74
International Journal of Medical Sciences And Clinical Research
https://theusajournals.com/index.php/ijmscr
inflammatory reactions in patients with acne. The main
representatives of the resident microbiome in acne
patients are Cutibacterium acnes (C. acnes) and
Staphylococcus epidermidis (S. epidermidis), while the
transient
microbiome
mainly
consists
of
Staphylococcus aureus (S. aureus) [1]. Based on the
results of scientific and clinical studies conducted in
recent years, it has been suggested that the
pathophysiology of inflammatory forms of acne is not
so much involved in known pathogens, but rather in the
imbalance of microorganisms living on the skin or
"dysbacteriosis" of the skin microflora [2].
Systemic antibiotics are used to treat moderate to
severe acne: minocycline, doxycycline, tetracycline and
erythromycin. All types of coagulase-negative and
Staphylococcus aureus are generally characterized by a
high frequency of isolation of resistant forms, a high
level and a wide spectrum of acquired resistance to
antibiotics. At the same time, the indicators of acquired
resistance in individual types of staphylococci, in
relation to different drugs and depending on the
habitat (hospital, community environment, surgical
hospitals) have significant differences, which should be
taken into account in the tactics of treatment and
prevention of diseases caused by them [5,6,8]. The aim
of the study was to evaluate the sensitivity of S.
epidermidis and S. aureus isolated from patients with
moderate to severe acne to systemic antibiotics,
namely
minocycline,
doxylane
(doxycycline),
tetracycline (tetracycline group) and erythromycin
(macrolide group) in the treatment of this pathology.
METHODS
The study material consisted of clinical samples taken
from patients with moderate (n= 42) and severe (n= 29)
acne. A total of 71 people were examined for this
purpose. Staphylococcal microflora was cultured from
facial skin using the print method and identified using
the standard scheme [3]. The culture was performed on
blood and yolk-salt agar (YSA). Antibiotic sensitivity of
isolated strains was determined using the disk diffusion
method on Mueller-Hinton agar in accordance with the
guidelines [9]. Statistical data processing was
performed using a program embedded in Microsoft
Excel.
RESULTS AND DISCUSSION
The assessment of the sensitivity of the isolated strains
of S. epidermidis and S. aureus to various antibiotics is
presented in diagrams 1 and 2.
Diagram 1
Sensitivity of S. epidermidis strains (n=23) to antimicrobial drugs isolated from
patients with moderate to severe acne, tetracycline and macrolide antibiotics (in %).
Diagram 2
Sensitivity of S. aureus strains (n=19) to antimicrobial drugs isolated from patients
with moderate and severe acne, tetracycline and macrolide antibiotics (in %).
26%
78%
61%
74%
Миноциклин
Доксилан
Тетрациклин
Эритромицин
International Journal of Medical Sciences And Clinical Research (ISSN: 2771-2265)
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International Journal of Medical Sciences And Clinical Research
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The results of the studies show that among the S.
epidermidis cultures (23 of 71), cultures resistant to
erythromycin were more common (74% - in 17
patients). The least resistance of the S. epidermidis
culture from the studied antibiotics was to tetracycline
(39% - 9), doxylan (26% - 6) and minocycline (22% - 5)
(diagram 3).
Diagram 3
Resistance of S. epidermidis strains (n=23) to antimicrobial drugs isolated from
patients with moderate and severe acne, to tetracycline and macrolide antibiotics (in
%).
All isolated S.aureus strains (in 19 patients) were
resistant to erythromycin (100%), which confirms the
complete acquisition of resistance of S.aureus to
erythromycin [7]. Resistance to tetracycline was 58%
(in 11 patients). Resistance to minocycline and
doxylane was at the level of -26% (in 5 patients) and
32% (in 65 patients), respectively (diagram 4).
0
42%
74%
68%
Миноциклин
Доксилан
Тетрациклин
Эритромицин
22%
74%
26%
39%
Миноциклин
Доксилан
Тетрациклин
Эритромицин
International Journal of Medical Sciences And Clinical Research (ISSN: 2771-2265)
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Diagram 4.
Resistance of S. aureus strains (n=19) to antimicrobial drugs isolated from patients
with moderate and severe acne, to tetracycline and macrolide antibiotics (in %).
The problem of antibiotic resistance of diseases caused
by staphylococci is still relevant. Year after year, their
resistance to ABP is becoming increasingly alarming.
CONCLUSIONS
1.
S. epidermidis and S. aureus strains were highly
sensitive to minocycline and doxylan.
2.
Erythromycin demonstrated the lowest efficacy
against S. epidermidis.
3.
All isolated S. aureus strains were characterized by
absolute resistance to erythromycin.
4.
The obtained data allow us to recommend
minocycline and doxylan for clinical use in the
treatment of patients with moderate to severe acne in
dermatological practice.
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