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Chemical Sciences
ASSEFOETIDA L., BY THE METHOD OF HIGH-PERFORMANCE LIQUID
CHROMATOGRAPHY
1
Iskandarova Sh.
2
Abdukhalilova N.
1
Iskandarova Shakhista, Professor, Tashkent Pharmaceutical Institute, Tashkent,
2
Abdukhalilova Nilufar, Assistant, Tashkent Pharmaceutical Institute, Tashkent,
Uzbekistan is a country endowed with a variety of medicinal plants with strong potential
for therapeutic application, Ferula assefoetida l. is called ferula is one of the most
popular medicinal herbs. It is important active ingredient responsible for the biological
activity of ferula, through the major activity is anti-inflammatory. This analysis was
aimed to determine analysis compound of ferulic acids analysis using high performance
liquid chromatography (HPLC), LC-20 Prominence, Shimadzu, column C-18 250
х
4,6
mm, 5 mcm; acetonitrile: acetic acid: aqua bides as mobile phase, flow rate 1 ml/min
and detection at 320 nm. Thus the analytical method using HPLC for ferulic acid were
feasible for quantitative analysis. As a result of the research, the content of dry extract
was 0,01% of the content of ferulic acid.
Keywords: Ferula assefoetida l., up-to-date circulating method, dry extract, HPLC,
standard sample, Ferulic acid
.
Ferula. (Ferula assefoetida L.). Ferula is an Umbrella family (Apiaceae). The Latin
name of the genus comes from lat. ferula-vine, rod [1]. Its main causative agent is ferulic
acid (3 methoxy-4-hydrochloric acid)- aromatic unsaturated carbonic acid, acid-acid
dressing. The name came from the Ferula plant (Ferula), which belongs to the family of
umbrellas. Appearance crystalline powder. Ferulic acid contains in the bark of many
plants and protect them from the sun, bacteria.
From a medical point of view, it has a wide range of pharmacological action. In
particular, it has an anti-inflammatory, anti-allergic, anti-tumor, anti-toxin,
hepatoprotector, cardioprotector, anti-microbial, anti-viral effectirga along with
antioxidant properties. As an antioxidant, it is part of many biologically active additives
and cosmetic agents [4]. In industry, ferulic acid is a synthetic aromatizer, the main raw
material in the production of vanillin.
Materials and methods. The research was carried out in high-performance liquid
chromatography, which was performed with the diode-matrix-
deteсtor
of the brand
name "Shimadzu" LC-20 Prominence (2016) model [2, 3].
Preparation of the controlled solution:
1 g (exactly) of the tested solution is taken
and transferred to a measuring tube with a volume of 100 ml. Hidrolized with 2,5
М
of
NaOH solution.
рН
of solution is leveled to 3.
Up to the mark was put 50 ml of water. It is processed in a ultra sound-bath. Then
added ethyl alcohol to the mark of the tube and mixed. Then was passed through a
membrane filter with diameter of hole 0,22
µm.
International Conference
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Conference Proceedings. Scope Academic House, January 30, 2021, Sheffield, UK.
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Preparation of a standard sample solution of ferulic acid:
The standard sample of
3,5 mg (exactly) ferulic acid is weighed and dissolved in acetonitrile, transferred to a
measuring tube in a volume of 50 ml.
Chromatography conditions:
Column:
С18,
250
х
4,6 mm, 5 mcm; detection: 320
nm; Mobile phase:acetonitrile and 0,5% acetic acid solution in a ratio of (38: 62); the
rate of flux is 1,0 ml/min; temperature 27
0
S; the volume of the sample that was injected
was 10
µL.
The controlled solution and the standard sample solution of ferulic acid are
chromatographed from 10
µL.
The amount of ferulic acid contained in the dry extract
(X, mg/g) is calculated according to the following formula:
𝑋 =
𝑆
1
× 𝑚
0
× 𝑃
𝑆 ⋅ 𝑚
1
× 1000
;
S
1-
the average surface of the pherolic acid precipitate calculated from the
chromatograms of the investigated solution;
m
0
- ferulic acid standard sample mass, mg;
Р
- the activity of the standard sample of ferulic acid,%.
S
0-
the average surface of the pherolic acid precipitate calculated from the
chromatograms of the standard sample solution of pherolic acid;
m
1
- the mass of the investigated ferulic acid, mg;
1-picture. Standard sample chromatogram
2-picture. Ferulic acid chromatogram, which is contained in the dry extract of ferula.
International Conference
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Conference Proceedings. Scope Academic House, January 30, 2021, Sheffield, UK.
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Conclusion. As a result of the research, the content of dry extract was 0,01% of
the content of ferulic acid. The Metrological description of the results obtained was
presented in Table 1.
1-table. Determination of the amount of ferulic acids
contained in the dry extract of ferula and its Metrological description
Load, g.
The amount of bioactive substances
detected, %
Metrological description
1,0124
0,01
Х
total.
=0.148
f=4 T=(95%, 4)=2.78
S
2
=0.00002
S=0.0039
S
x
=0.0017
Е
total.
=33.28
1,0017
0,012
1,0109
0,015
1,0102
0,017
1,0058
0,02
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