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PREPARATION OF WATER-INSOLUBLE FILMS BASED ON SILK
FIBROIN AND POLYSACCHARIDE.
Sodiqova M.A.
Karimov A.
SHomurotov Sh.A.,
Institute of Bioorganic Chemistry of the Academy of
Sciences of the Republic of Uzbekistan
Tashkent Pharmaceutical Institute, Tashkent, Uzbekistan
e-mail; mohinursodiqova347@gmail.com (+998908249992)
https://doi.org/10.5281/zenodo.11656820
Complexity: it is possible to obtain compositions by connecting fibroin
protein extracted from silkworms with various polysaccharides. From aqueous
solutions of silk fibroining, films with varying parameters and characteristics are
obtained. Thus, for example, nanoscale fibroin films can be obtained from
aqueous solutions using the "layer-by-layer" technique..
This technique allows obtaining biopolymer coatings of the given size and
thickness. Nano-sized films perfectly support the adhesion and proliferation of
mesenchymal stem cells..
The aim of the study:
It was shown that the growth and reproduction of
fibroblasts in fibroin films is the same as in collagen films
.
Other mammalian and insect cells have also been shown to adhere better to
fibroin coatings compared to collagen films.
Chemically modified fibroin films improve cell attachment and bone formation.
Methods and methods: fibrous materials made of polysaccharides and proteins
are exposed to the natural environment of the div and thus provide favorable
conditions for the growth and renewal of tissues. In this study, we obtained
compositions based on fibroin-polysaccharide. Based on the above methods, a
composite material in the form of film was obtained based on purified fibroin
and dextran (dialdehydedextran). For this, a periodontal oxidation reaction with
dextran was carried out, and dialdehyde products containing 10-90% oxidized
links were obtained. It was determined that the molecular parameters of
aldehyde dextran can be changed by changing the reaction conditions and the
amount of aldehyde groups. The resulting dialdehyde products are reacted with
primary amines to form azomethine-bonded compounds that are easily
hydrolyzed. This allows them to be used as a polymer carrier in the preparation
of long-acting macromolecular drug systems..
Results:
Films prepared from aqueous solution of fibroin and dextran were
obtained by casting. The fibroin and dextran solution was poured into Petri
SOLUTION OF SOCIAL PROBLEMS IN
MANAGEMENT AND ECONOMY
International scientific-online conference
74
dishes with a diameter of 35 mm. 50 μm thick films were formed in Petri dishes.
Films were dried at room temperature, then washed with ethanol and
rehydrated with double distilled water.. To study the proliferative activity of
cells, it is necessary to take into account the morphofunctional state of the
product surface based on pure fibroin (fiber, matrix or hydrogel, depending on
the material). Scanning electron microscopy and atomic force interference
microscopy are often used as microscopy methods. One of the most universal
parameters describing surface-cell adhesion is the value of the material's surface
energy. Compared with other fibrillar proteins, an important feature of silk
fibroin as a biomaterial is the versatility of sterilization options.
Conclusions:
The film forming process was carried out as follows. An aqueous
solution was prepared on the basis of fibroin and dialdehyde dextran separated
by the above method. Their proportions were carried out in the proportions 1/1,
1/2, 1/3, 1/4, 1/5, and the concentration of the solution was 30 mg/ml. The
solutions prepared in these proportions were poured into petri dishes and left
to dry for 3 days at room temperature. Prepared films were washed twice with
ethyl alcohol and removed from the Petri dish using a scalpel.
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фброинов 3d каркасах," Biomaterials, vol. 30, no. 15, May 2009.
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