Authors

  • Garcia Shnyrov
    Departamento De Quimica Fisica, Universidad De Salamanca, Spain

DOI:

https://doi.org/10.37547/tajabe/Volume03Issue08-04

Keywords:

Oxidase Plant Regeneration

Abstract

The present work describes a study on the isolation, purification and a few features of a completely unique peroxidase from white Spanish broom, a tree legume very abundant within the northern half Spain and Portugal. Optimal conditions are proposed for enzyme extraction, removal of phenolic compounds and enzyme purification by consecutive hydrophobic, ion-exchange and size-exclusion chromatographies. Peroxidase from white broom was found to possess a relative molecular mass of 49 kDa. The spectrum of CMP showed a Soret band at 403 nm with a Rz factor of three .3. Substrate specificity and therefore the effect of some variables on the activity of CMP with guaiacol as cosubstrate have also been investigated.

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