This study investigates the antioxidant properties of Coscinium fenestratum stem extracts in a streptozotocin-induced type 1 diabetes rat model. Diabetes mellitus is associated with oxidative stress and impaired antioxidant defense mechanisms, leading to various complications. Coscinium fenestratum, a medicinal plant rich in bioactive compounds, has shown potential antioxidant effects. In this research, type 1 diabetes was induced using streptozotocin in rats, followed by the administration of Coscinium fenestratum stem extracts. The extracts' antioxidant potential was evaluated through measurements of reactive oxygen species levels, enzymatic antioxidants (superoxide dismutase, catalase, glutathione peroxidase), and non-enzymatic antioxidants (glutathione, vitamin C, vitamin E). Histological assessments of pancreatic tissues were also conducted. The results demonstrate that Coscinium fenestratum extracts significantly reduce oxidative stress markers and enhance antioxidant defenses in diabetic rats. Moreover, histological improvements in pancreatic tissues were observed. These findings suggest that Coscinium fenestratum stem extracts hold promise as a therapeutic intervention for combating oxidative stress-associated complications in type 1 diabetes.
Aebi H .Catalase in Vitro. Methods of Enzymol,1984; 105:121-126.
Darrow, R. A., and S. P. Colowick. 1962. Hexokinase from baker’s yeast. In: Methods in Enzymology No. 5. S. P. Colowick and N. O. Kaplan, ed. Academic Press Inc., New York. p.226-227.
Fridovich I. The biology of oxygen radicals. Science, 1978; 201: 875–880.
Habig WH, Pabst MJ, Jakoby WB . Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem ,1974;246:7130-7139
Halim EM. Lowering of blood sugar by water extract of Azadirachta indica and Abroma augusta in diabetic rats. Indian J.Exp.Bio,2003; 41: 636-640.
John WB. Role of oxidative stress in development of complications in diabetes. Diabetes , 1991; 40: 405.
Johnson F and Giulivi C. Superoxide dismutase and their impact on human health. Mol aspects Med, 2005; 26 (4-5):340-52
Indran M, Rokiah P, Chan SP, Kuppusamy UR .Alteration of lipid peroxidation and antioxidant enzymes in young Malaysian IDDM patients. Med J Malaysia,2004;59:166-70.
Mathieu B, Stefaan K, Willy S. Specific features of glycogen metabolism in the liver. Biochem.J 1998; 336:19-31
Pari L. and Satheesh A.M. Antidiabetic effect of Boerhavia diffusa : effect on serum and tissue lipids in experimental diabetes. J. Med. Food, 2004; 7: 472-476.
Pari L, Umamaheswari J. 2000. Antihyperglycemic activity of Musa sapientum flowers: effect on lipid peroxidation in alloxan diabetic rats. Phytother Res 14(2)136-138.