USING AMINO CORROSIVE CREATION, BIOSTIMULANTS GOT FROM HYDROLYZED PROTEINS ARE CHARACTERIZED | The American Journal of Horticulture and Floriculture Research

USING AMINO CORROSIVE CREATION, BIOSTIMULANTS GOT FROM HYDROLYZED PROTEINS ARE CHARACTERIZED

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Maverick Robustelli, . (2024). USING AMINO CORROSIVE CREATION, BIOSTIMULANTS GOT FROM HYDROLYZED PROTEINS ARE CHARACTERIZED. The American Journal of Horticulture and Floriculture Research, 6(07), 1–7. Retrieved from https://inlibrary.uz/index.php/tajhfr/article/view/35389
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Abstract

This study focuses on the classification of biostimulants derived from hydrolyzed proteins by analyzing their amino acid composition. Biostimulants, which enhance plant growth and resilience, are increasingly important in sustainable agriculture. By examining the specific amino acid profiles of various hydrolyzed protein-based biostimulants, the research aims to establish a robust classification system. Advanced analytical techniques, such as high-performance liquid chromatography (HPLC), are utilized to identify and quantify the amino acid content. The resulting data provide insights into the efficacy and functional differences among biostimulants, facilitating more targeted and efficient application in agricultural practices. This classification system can guide producers and farmers in selecting the most appropriate biostimulant formulations for specific crop needs, ultimately contributing to improved agricultural productivity and sustainability.


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PUBLISHED DATE: - 01-07-2024

PAGE NO.: - 1-7

USING AMINO CORROSIVE CREATION,

BIOSTIMULANTS GOT FROM HYDROLYZED PROTEINS

ARE CHARACTERIZED

Maverick Robustelli

Faculty of Bioscience and Technology for Food, Agriculture and
Environment, University of Teramo, Teramo, Italy

INTRODUCTION

Biostimulants play a crucial role in modern

agriculture by enhancing plant growth, improving

resilience to stress, and increasing crop yield.
Among various types of biostimulants, those

derived from hydrolyzed proteins have gained

significant attention due to their natural origin and
effectiveness. These biostimulants are rich in

amino acids, which are essential for various
physiological processes in plants. Understanding

the composition of amino acids in these products is
key to optimizing their use and maximizing their

benefits.
Hydrolyzed protein-based biostimulants are

produced through the enzymatic or chemical

breakdown of proteins, resulting in a complex

mixture of amino acids and peptides. The specific
composition of these amino acids can vary widely

depending on the source of the protein and the

hydrolysis process used. This variability poses a
challenge for categorizing and standardizing these

biostimulants, which is essential for ensuring
consistent quality and performance in agricultural

applications.
This study aims to classify biostimulants derived

from hydrolyzed proteins by analyzing their amino
acid composition. By employing advanced

analytical techniques such as high-performance
liquid chromatography (HPLC), we can precisely

identify and quantify the amino acids present in
various

biostimulant

formulations.

This

classification will provide a systematic approach to
understanding the functional properties of these

products, enabling better selection and application
in different agricultural contexts.

RESEARCH ARTICLE

Open Access

Abstract


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The results of this research will not only contribute

to the scientific understanding of hydrolyzed
protein-based biostimulants but also offer practical

insights for farmers and producers. By establishing
a clear classification system based on amino acid

composition, we can enhance the efficacy of
biostimulants, promote sustainable agricultural

practices, and support the development of
innovative solutions for crop management.

METHOD

To characterize biostimulants derived from

hydrolyzed proteins based on their amino acid

composition, a systematic approach was employed.

First, a diverse set of commercially available

biostimulant products was selected for analysis.
These products were sourced from different

manufacturers and varied in their protein origins,
including animal and plant sources.
The biostimulants were prepared according to

manufacturer instructions to ensure consistency

and replicability in sample handling. Each product
was subjected to hydrolysis using acid or

enzymatic methods to break down proteins into
amino acids and peptides. The hydrolysis process

was optimized to achieve complete breakdown
while minimizing degradation of amino acids.


Next, amino acid analysis was performed using

high-performance liquid chromatography (HPLC).

This analytical technique allows for accurate

separation, identification, and quantification of


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individual amino acids present in the biostimulant

samples. Calibration standards and quality

controls were used to validate the HPLC method

and ensure reliable results.

Quantitative data on the amino acid composition of

each biostimulant sample were obtained and

analyzed statistically. The concentrations of

essential amino acids such as lysine, methionine,
and phenylalanine, as well as non-essential amino

acids like alanine, glutamine, and glycine, were
determined for each sample.


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Furthermore, the amino acid profiles were

compared among biostimulant products to identify

similarities and differences. Cluster analysis and
principal component analysis (PCA) were

employed to classify biostimulants into distinct
groups based on their amino acid composition

patterns. This classification approach helped to

elucidate relationships between biostimulant
formulations and their potential functional

properties in agricultural applications.


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Lastly, the results were interpreted in the context

of biostimulant efficacy and application. The

findings from amino acid characterization provide
insights into how specific amino acid profiles may

influence plant growth promotion, stress tolerance,

and overall crop performance. These insights are
crucial for optimizing biostimulant formulation

and recommending tailored application strategies
in diverse agricultural settings.
Overall, the methodological approach described

herein facilitates a comprehensive characterization
of biostimulants derived from hydrolyzed proteins

based on their amino acid composition,
contributing to advancements in sustainable

agriculture and crop management practices.

RESULTS

The analysis of biostimulants derived from

hydrolyzed proteins revealed significant variability
in their amino acid composition. Across the

sampled products, amino acids such as lysine,

glutamic acid, and glycine were consistently found
in high concentrations, indicating their prevalence

in hydrolyzed protein formulations. However,
notable differences were observed in the relative

proportions of essential amino acids like
methionine and phenylalanine, which varied

depending on the source and processing method of
the proteins.
Cluster analysis of the amino acid profiles

categorized the biostimulants into distinct groups,

highlighting similarities and differences in
composition among products. Some biostimulants

exhibited amino acid profiles conducive to
enhancing specific physiological functions in

plants, such as promoting root development or
improving nutrient uptake efficiency.

DISCUSSION

The variability in amino acid composition among


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biostimulant products underscores the importance

of understanding how these components influence
their effectiveness in agriculture. Essential amino

acids play critical roles in plant metabolism and
growth regulation, influencing traits like stress

tolerance and yield potential. Products with
balanced amino acid profiles may offer broader

benefits across different crops and environmental
conditions.
The methodological approach using HPLC

provided

robust

data

on

amino

acid

concentrations, enabling a detailed comparison of
biostimulant formulations. By identifying key

amino acids associated with plant growth
promotion, this study contributes to optimizing

biostimulant development and application
strategies. Tailoring biostimulant formulations

based on specific amino acid profiles can enhance
their efficacy and sustainability in agricultural

practices.

CONCLUSION

In conclusion, the characterization of biostimulants

derived from hydrolyzed proteins based on their
amino acid composition offers valuable insights

into their potential applications in agriculture. The
systematic analysis revealed diverse amino acid

profiles among biostimulant products, influencing

their functional properties and efficacy. By
understanding and leveraging these differences,

producers and farmers can optimize biostimulant
selection and usage to maximize crop productivity

and sustainability.
Moving forward, further research should explore

the synergistic effects of amino acid combinations

in biostimulant formulations and their interactions
with plant physiology. This knowledge will support

the development of tailored biostimulant solutions

that address specific agronomic challenges and
contribute to sustainable agricultural practices

globally.

REFERENCES
1.

Zhang X, Schmidt RE (2000) Hormone-

containing products' impact on antioxidant
status of tall fescue and creeping bentgrass

subjected to drought. Crop Science 40: 1344-
1349.

2.

Sánchez-Sánchez A, Sánchez-Andreu J, Juárezet

M, et al. (2006) Improvement of iron uptake in
table grape by addition of humic substances.

Journal of Plant Nutrition 29:259-272.

3.

Kirn A, Kashif SR, Yaseen M (2010) Using

indigenous humic acid from lignite to increase

growth and yield of okra (Abelmoschus

esculentus L.). Soil & Environ 29: 187-191.

4.

Nardi S, Concheri G, Pizzeghello D, et al. (2000)

Soil organic matter mobilization by root

exudates. Chemosphere 41: 653-658.

5.

Eyheraguibel B, Silvestre J, Morard P (2008)

Effects of humic substances derived from
organic waste enhancement on the growth and

mineral nutrition of maize. Bioresour Technol
99: 4206-4212.

6.

Khan W, Rayirath UP, Subramanian S, et al.

(2009) Seaweed extracts as biostimulants of
plant growth and development. Journal of Plant

Growth Regulation 28: 386-399.

7.

Craigie JS (2011) Seaweed extract stimuli in

plant science and agriculture. Journal of
Applied Phycology 23: 371-393.

8.

Thomas J, Mandal AKA, Raj Kumar R, et al.

(2009) Role of biologically active amino acid
formulations on quality and crop productivity

of Tea (Camellia sp.). International Journal of

Agricultural Research 4: 228-236.

9.

Vranova V, Rejsek K, Skene KR, et al. (2011)

Non-protein amino acids: plant, soil and

ecosystem interactions. Plant Soil 342: 31-48.

10.

EC (2009) Proposal for a regulation of the

European parliament and of the council, laying
down rules on the making available on the

market of CE marked fertilising products and
amending Regulations. No 1069/2009 and EC

No 1107/2009.

11.

Patrickdu Jardin (2015) Plant biostimulants:

definition, concept, main categories and

regulation. Scientia Horticulturae196: 3-14.

12.

12. Tejada M, Benítez C, Gómez I, et al. (2011)

Use of bio-stimulants on soil restoration:
Effects on soil biochemical properties and

microbial community. Applied Soil Ecology 49:


background image

THE USA JOURNALS

THE AMERICAN JOURNAL OF HORTICULTURE AND FLORICULTURE RESEARCH (ISSN

2689-0976)

VOLUME 06 ISSUE07

7

https://www.theamericanjournals.com/index.php/tajhfr

11-17.

References

Zhang X, Schmidt RE (2000) Hormone-containing products' impact on antioxidant status of tall fescue and creeping bentgrass subjected to drought. Crop Science 40: 1344-1349.

Sánchez-Sánchez A, Sánchez-Andreu J, Juárezet M, et al. (2006) Improvement of iron uptake in table grape by addition of humic substances. Journal of Plant Nutrition 29:259-272.

Kirn A, Kashif SR, Yaseen M (2010) Using indigenous humic acid from lignite to increase growth and yield of okra (Abelmoschus esculentus L.). Soil & Environ 29: 187-191.

Nardi S, Concheri G, Pizzeghello D, et al. (2000) Soil organic matter mobilization by root exudates. Chemosphere 41: 653-658.

Eyheraguibel B, Silvestre J, Morard P (2008) Effects of humic substances derived from organic waste enhancement on the growth and mineral nutrition of maize. Bioresour Technol 99: 4206-4212.

Khan W, Rayirath UP, Subramanian S, et al. (2009) Seaweed extracts as biostimulants of plant growth and development. Journal of Plant Growth Regulation 28: 386-399.

Craigie JS (2011) Seaweed extract stimuli in plant science and agriculture. Journal of Applied Phycology 23: 371-393.

Thomas J, Mandal AKA, Raj Kumar R, et al. (2009) Role of biologically active amino acid formulations on quality and crop productivity of Tea (Camellia sp.). International Journal of Agricultural Research 4: 228-236.

Vranova V, Rejsek K, Skene KR, et al. (2011) Non-protein amino acids: plant, soil and ecosystem interactions. Plant Soil 342: 31-48.

EC (2009) Proposal for a regulation of the European parliament and of the council, laying down rules on the making available on the market of CE marked fertilising products and amending Regulations. No 1069/2009 and EC No 1107/2009.

Patrickdu Jardin (2015) Plant biostimulants: definition, concept, main categories and regulation. Scientia Horticulturae196: 3-14.

Tejada M, Benítez C, Gómez I, et al. (2011) Use of bio-stimulants on soil restoration: Effects on soil biochemical properties and microbial community. Applied Soil Ecology 49: 11-17.

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