Authors

  • S.I. Sadikova
    Associate Professor, Department Of Internal Medicine, Family Medicine No. 2, Tashkent Medical Academy, Tashkent, Uzbekistan
  • N. S.- Khodjaeva
    Doctor Of The Highest Category Of Ultrasound Diagnostics At The Akfa University Clinicmedline, Tashkent, Uzbekistan

DOI:

https://doi.org/10.37547/TAJMSPR/Volume06Issue06-02

Keywords:

Non-alcoholic fatty liver disease insulin resistance atherosclerosis

Abstract

In the context of insulin resistance syndrome, which encompasses changes in lipid and carbohydrate metabolism, liver-related issues are now being closely examined alongside cardiovascular diseases. Our research aimed to evaluate the predictive importance of non-alcoholic fatty liver disease (NAFLD) for identifying the likelihood of early atherosclerotic alterations in the carotid arteries. We discovered a connection between NAFLD and initial signs of atherosclerosis. By ranking prognostic factors that affect changes in the carotid artery wall, we've been able to develop a method for estimating individual atherosclerosis risk in people who are otherwise healthy. The practical value of our findings underscores the need for in-depth evaluation of individuals with NAFLD to identify risk factors that may contribute to the advancement of both liver and cardiovascular diseases.


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PUBLISHED DATE: - 14-06-2024

DOI: -

https://doi.org/10.37547/TAJMSPR/Volume06Issue06-02

PAGE NO.: - 08-17

THE CONTRIBUTION OF NON-ALCOHOLIC
FATTY LIVER DISEASE TO THE
PROGRESSION OF ATHEROSCLEROSIS


S.I. Sadikova

Associate Professor, Department Of Internal Medicine, Family Medicine No.
2, Tashkent Medical Academy, Tashkent, Uzbekistan

N. S.- Khodjaeva

Doctor Of The Highest Category Of Ultrasound Diagnostics At The Akfa

University Clinicmedline, Tashkent, Uzbekistan

INTRODUCTION

Non-alcoholic fatty liver disease (NAFLD) is now

identified as a leading chronic liver condition

worldwide[1]. Despite the challenge in pinpointing
precise incidence rates, estimates suggest that

NAFLD affects 20

30% of populations in Western

countries and 5

18% in Asian countries, with these

numbers increasing sharply over time. It's believed
that NAFLD impacts about 25

30% of people

globally[2].

This

condition,

along

with

cardiovascular

diseases

and

metabolic

disturbances in lipid and carbohydrate metabolism

associated with insulin resistance (IR) syndrome, is
gaining increased attention. NAFLD covers a broad

range of metabolic liver damages in the context of
IR, including simple fatty liver (steatosis), steatosis

with inflammation and liver cell damage (non-
alcoholic steatohepatitis, NASH), and fibrosis,

which can evolve into cirrhosis of the liver[3, 4].
The outlook for individuals diagnosed with NAFLD

is concerning; about 40% of those with simple
steatosis progress to NASH within 8-13 years, and

from this group, 15% may develop liver cirrhosis
and liver failure. Moreover, 7% of patients with

liver cirrhosis are at risk of developing
hepatocellular carcinoma (HCC) within a

decade[5]. The expectation is that NAFLD will soon
become the predominant liver disorder, already

RESEARCH ARTICLE

Open Access

Abstract


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being the second leading cause of HCC and liver
transplants[6].
Research has highlighted a significant link between

the onset of non-alcoholic fatty liver disease

(NAFLD) and several metabolic conditions, such as
obesity, type 2 diabetes mellitus (T2DM), and

atherogenic dyslipidemia, particularly in cases
where insulin resistance (IR) is present. Within the

general European population, the occurrence rate
of NAFLD is found to be between 20-33%. This rate

escalates among individuals diagnosed with type 2
diabetes mellitus, where it is observed to vary from

42.6-69%. This data underscores the intertwined

nature of metabolic diseases and the critical role of
insulin resistance in the development of NAFLD[7,

8].
Furthermore, there's an ongoing dialogue around

genetic mutations affecting carbohydrate and lipid

metabolism, which result in changes to insulin
sensitivity within the liver[9; 10]. These genetic

factors contribute to the broader understanding of
NAFLD's pathogenesis and its strong links to other

symptoms of insulin resistance (IR). This

connection positions non-alcoholic fatty liver
disease (NAFLD) as a liver-centered component of

metabolic syndrome[11; 12]. Yet, as the liver
engages in this pathological sequence, it transitions

from being merely an affected organ to an active
participant that intensifies the metabolic

imbalances associated with IR.
Studies have shown that disruptions in insulin

breakdown and glucose usage occur in the liver

during fatty hepatosis, leading to an environment

conducive to the production of atherogenic
cholesterol fractions and triglycerides (TG). These

disruptions

are

instrumental

in

causing

disturbances

in

carbohydrate

and

lipid

metabolism, precipitating the early emergence of
atherosclerosis and subsequent cardiovascular

diseases[13; 14]. Research by Natadisa M. in 2007
further illuminates the heightened risk of

atherosclerosis in individuals with NAFLD, which is
found to be 4.12 times greater than in those

without the disease, as indicated by a 95%
confidence interval (CI) of 1.58-10.75 and a

significance level of p=0.004. Additionally, the

study highlights a gender disparity in the risk of
cardiovascular complications associated with

NAFLD, with women facing a risk 7.32 times higher

compared to men's 3.56 times, showcasing a
significant difference (p<0.027)[15].
Atherosclerosis is identified as a condition that

involves the liver, where it's established that two
main factors are essential for its onset: lipid

metabolism disorders and vascular endothelium
damage. Within the scope of NAFLD, the

disturbance in lipid metabolism is evidenced by
increased low-density lipoprotein cholesterol

(LDL-C) levels, decreased high-density lipoprotein

cholesterol (HDL-C) levels, and the presence of
hypertriglyceridemia. The harm to arterial

endothelium associated with liver diseases stems
from various factors, including oxidized LDL lipids,

elevated C-reactive protein (CRP) levels,
heightened activity of lipoprotein-associated

phospholipase

A2,

hyperglycemia,

insulin

resistance, raised homocysteine levels, increased

fibrinogen levels, and a scarcity of nitric oxide
(NO). These elements collectively contribute to the

complex interplay between NAFLD and the
development of atherosclerosis [16].
Enhanced arterial stiffness in individuals with non-

alcoholic fatty liver disease (NAFLD) is garnering

notable focus, positioning NAFLD as a marker for
this condition[24]. This increased stiffness of

arteries, which persists even when traditional
cardiovascular risk factors are accounted for, is

linked with NAFLD. Patients with NAFLD show
reduced elasticity and flexibility of the aorta,

indicating that both the presence and severity of
NAFLD correlate with heightened arterial stiffness,

regardless of the existence of hypertension (HTN)
or diabetes mellitus (DM) [17].
In the "Cardio-GOOSE" study, the assessment of

arterial stiffness was conducted through the

measurement of carotid-femoral pulse wave
velocity (PWV) and detection of subclinical

atherosclerosis via intima-media thickness (IMT).
Results showed no significant difference in IMT

between those with and without NAFLD
(0.77±0.15

mm

versus

0.76±0.14

mm,

respectively) [18]. However, for individuals


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diagnosed with both NAFLD and metabolic
syndrome (MS), IMT values were noticeably higher

(0.85±0.16 mm; p<0.005) compared to those

without MS. Furthermore, an increase in vascular
wall stiffness was observed in patients with

NAFLD, especially pronounced in those also
suffering from MS, with the PWV measurements

being higher in the NAFLD+MS group (8.29±2.2
m/s; p<0.001). The occurrence of NAFLD was more

common in participants exhibiting greater vascular
stiffness, even after adjusting for MS (p<0.05),

highlighting the intricate relationship between
NAFLD and cardiovascular health [19].
Non-alcoholic fatty liver disease (NAFLD) is linked

to a heightened risk of atherosclerosis, including its

asymptomatic stages, highlighted by increased
intima-media thickness (IMT) and raised levels of

C-reactive protein (CRP). According to research
conducted by Kim in 2009, the IMT in patients with

NAFLD was found to be 0.034 mm thicker than in
those without the condition, a difference that was

statistically

significant

(p=0.016)

[20].

Additionally, individuals with NAFLD are more

likely to have silent atherosclerotic changes in the
carotid arteries. The occurrence of atherosclerotic

plaques is notably higher among patients with
NAFLD, with a prevalence of 57.8%, compared to

37.5% in those without NAFLD (p=0.02).

Furthermore, the likelihood of developing carotid
atherosclerosis in patients with NAFLD is

increased by 1.85 times (p<0.001), underscoring
the significant impact of NAFLD on cardiovascular

health and the importance of monitoring for
atherosclerotic changes in this patient population

[21].
Over a span of 21 years, cardiovascular diseases

have been identified as the leading cause of

mortality among individuals diagnosed with non-

alcoholic fatty liver disease (NAFLD). Additionally,
NAFLD is linked to a rise in not just cardiovascular-

related deaths but also overall mortality rates. This
interplay of factors considerably hastens the onset

and advancement of atherosclerosis and its related
cardiovascular conditions. As a result, investigating

NAFLD as a separate and contributory risk factor in
the progression of atherosclerosis has become a

focal point of contemporary research, highlighting
the importance of recognizing and managing

NAFLD to potentially mitigate the risk of

cardiovascular complications [22].
Purpose.Our research aimed to evaluate the

predictive value of non-alcoholic fatty liver disease

(NAFLD) in identifying the likelihood of early signs
of atherosclerotic vascular damage, with a specific

focus on the lower extremities. This study sought
to understand how NAFLD could serve as an

indicator for the onset of atherosclerosis in these
areas, potentially offering insights into the broader

implications of NAFLD on cardiovascular health

and the importance of early detection and
management of vascular risks associated with this

liver condition.

MATERIAL AND METHODS OF RESEARCH

Our investigation encompassed 100 individuals,

aged 35 to 45 years, who were participating in
standard health screenings at the "Akfa Medline"

university clinic in Tashkent. These participants
were asymptomatic at the time of their evaluation.

The study set specific exclusion criteria to ensure a
homogeneous sample population, disqualifying

individuals with obesity (defined by a div mass
index not exceeding 30 kg/m^2), hypertension,

coronary artery disease, type 2 diabetes mellitus,
and renal or gastrointestinal diseases that

necessitated drug treatment. This approach aimed
to isolate the impact of non-alcoholic fatty liver

disease (NAFLD) on the early development of
atherosclerotic vascular changes in the lower

extremities, minimizing confounding factors that

could influence the outcomes.
In gathering data on alcohol consumption history,

our methodology adhered to the guidelines set

forth by the World Health Organization (WHO)
from the year 2000. We specifically focused on

alcohol intake levels and only considered
consumption patterns that exceeded WHO's

recommended norms if they occurred within the
last five years; any excessive alcohol consumption

more than 5 years prior was not included in our

analysis. Additionally, we meticulously excluded
any participants with a history of viral hepatitis or


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liver

damage

attributed

to

toxins

or

pharmaceuticals. This careful selection process

aimed to ensure the accuracy of our study by

eliminating potential confounding factors related
to liver health and focusing on the impact of non-

alcoholic factors on liver disease.
A comprehensive physical examination of the

participants was carried out, which included the

measurement of key anthropometric indicators
and blood pressure levels to assess overall health

status. In addition, a detailed analysis of lipid
profile markers was performed. This analysis

involved determining the levels of total cholesterol

(TC), high-density lipoprotein cholesterol (HDL-C),
triglycerides (TG), and low-density lipoprotein

cholesterol (LDL-C). These measurements were
crucial for evaluating the metabolic and

cardiovascular health of the participants, providing
essential insights into their risk factors for

developing conditions such as atherosclerosis and
cardiovascular diseases, particularly in the context

of non-alcoholic fatty liver disease (NAFLD).
The evaluation of the intima-media thickness (IMT)

of the common carotid arteries (CCA) was executed
employing a standardized approach on the

ACUSON S2000 and S3000 2019 ultrasound
machines, which were outfitted with a linear probe

that utilizes a phased array technology at a
frequency of 7.5 MHz. This examination targeted

the CCA at three distinct locations, specifically 2 cm
below the bifurcation point on both the right and

left sides of the neck. To derive the average IMT
value for the CCA, measurements from these six

points were aggregated. The criteria for identifying
early indications of atherosclerosis involved

detecting a localized increase in the CCA IMT to
more than 1.5 mm at any measured site along the

carotid artery, denoted as the maximum CCA IMT.

This measurement process is critical for diagnosing
the preliminary stages of atherosclerosis, offering

valuable insights into the cardiovascular risk
profile of the study participants.
All participants in the study were subjected to a

liver ultrasound examination to assess various
aspects of liver health, including the oblique

vertical size of the liver (OVS), the density of the

liver parenchyma, the condition of the liver bile
ducts, and the vascular pattern. For capturing

detailed images of the liver parenchyma,

performing measurements of its lobes, and
evaluating its structure, the Acuson Sequoia Expert

system 2022 was employed. This advanced
ultrasound system is equipped with a convex probe

that features a phased array with a frequency of 3.5
MHz, making it particularly suited for B-mode

scanning of internal organs.
The liver ultrasound procedure was carried out in

accordance with the standard methodological

guidelines as outlined by V. V. Mitkov in 2007. This

ensured that the examination was performed
consistently and accurately across all patients,

allowing for the reliable assessment of liver health
and the identification of potential indicators of

non-alcoholic fatty liver disease (NAFLD) or other
liver-related conditions.
The evaluation of the liver's architecture and

vascular pattern was conducted meticulously. The
diagnosis of fatty infiltration within the liver was

determined through a comprehensive assessment,

which included examining the oblique vertical size
(OVS) of the liver, its echogenicity, the vascular

pattern, and the sound conduction properties of
the liver parenchyma. These criteria are crucial for

identifying the presence and extent of fatty
infiltration, as changes in echogenicity and sound

conduction often indicate increased fat deposits
within the liver tissue. Moreover, alterations in the

vascular pattern can suggest changes in liver
density and structure associated with fatty liver

disease. This approach allows for a detailed
understanding of liver health and the identification

of non-alcoholic fatty liver disease (NAFLD) or
other conditions that might impact the liver's

function and structure.
The analysis of statistical data was carried out

using the SPSS software, version 11.0, a standard
package for statistical analysis. Quantitative data

were summarized using the mean value and the
standard error of the mean (M ± m), providing a

clear depiction of the central tendency and
variability within the data. To determine the

significance of the differences observed between


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various groups in the study, the Student's t-test
was employed, a widely used method for

comparing means.
Furthermore, to ascertain the prognostic relevance

of the characteristics under study, a multivariate
stepwise regression analysis was performed. This

advanced statistical method allows for the
identification of the most significant predictors

among a set of variables, thereby understanding
their combined effect on a particular outcome. The

threshold for statistical significance was set at a
probability level of p < 0.05, meaning that results

with a p-value less than 0.05 were considered

statistically significant. This rigorous analytical
approach ensured that the findings of the study

were both reliable and valid, contributing valuable
insights into the prognostic significance of various

factors in relation to the health conditions being
investigated.

RESEARCH RESULTS

In our study, fatty infiltration of the liver

parenchyma was identified in 48 participants, who

were then categorized into the primary study
group. This group exhibited increased echogenicity

within the altered liver parenchyma, which was
indicative of fatty infiltration. Additionally, an

acoustic phenomenon of ultrasound attenuation
was noted in the deeper layers of the parenchyma,

suggesting alterations in the liver's structure due to
fat accumulation. Despite these changes, the liver

parenchyma's structure appeared homogeneous,
and there were no alterations in the organ's shape;

the liver maintained smooth contours and a sharp

edge, indicating no significant morphological
distortion.
The oblique vertical size (OVS) of the right liver

lobe emerged as a particularly informative and
widely accepted measure for assessing liver health.

Within the NAFLD group, the OVS of the right liver
lobe in 12 patients (accounting for 27.3% of the

group) exceeded the established threshold values,
measuring more than 140 mm, signaling significant

liver enlargement.
For comparison, a control group was established,

comprising 52 individuals who showed no

ultrasound evidence of NAFLD. This distinction
between the groups based on ultrasound findings

of the liver allowed for a comparative analysis,

further highlighting the impact of fatty infiltration
on liver structure and function.
The study ensured that the groups were well-

matched in terms of gender, age, Body Mass Index
(BMI), and alcohol consumption history,

facilitating a reliable comparison. Specifically,
within both the primary and control groups, a

similar percentage of patients reported low-dose
alcohol consumption, with 54.6% in the main

group and 58.9% in the control group. The average

age of participants was 41.1 ± 2.1 years in the main
group and 37.2 ± 1.9 years in the control group,

showing no statistically significant difference
between the two (p = 0.172).
Among the patients with non-alcoholic fatty liver

disease (NAFLD), the fasting blood glucose level
was notably higher at 5.35 ± 0.07 mmol/L, in

contrast to the control group's average of 5.05 ±
0.07 mmol/L (p = 0.006), indicating a significant

shift in carbohydrate metabolism. Indeed, fasting

hyperglycemia was present in 45.5% of the NAFLD
group compared to 26.8% in the control group (p =

0.010), highlighting a marked difference in glucose
regulation.
Furthermore, alterations in lipid profile indicators

were significantly more prevalent among patients
with NAFLD. The average total cholesterol (TC)

level in the NAFLD group was notably higher than
the norm and exceeded that of the control group, at

5.96 ± 0.21 mmol/L versus 5.11 ± 0.15 mmol/L,

respectively (p = 0.001). Triglyceride (TG) levels
were also substantially elevated in the NAFLD

group, at 1.72 ± 0.20 mmol/L, compared to 0.81 ±
0.05 mmol/L in the control group (p < 0.001).

Additionally, low-density lipoprotein cholesterol
(LDL-C) levels were higher in the main group, at

3.89 ± 0.20 mmol/L, versus 3.24 ± 0.12 mmol/L in
the control group (p = 0.004), underscoring

significant differences in lipid metabolism between
those with and without NAFLD.
In both groups, levels of high-density lipoprotein

cholesterol (HDL-C) were found to be within


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normal ranges. However, in the group with non-
alcoholic fatty liver disease (NAFLD), a significant

40.9% of patients showed lipid profile changes that

exceeded the threshold for atherogenic cholesterol
fractions, a stark contrast to only 1.8% in the

control group (p < 0.001). This indicates a
markedly higher prevalence of atherogenic lipid

profiles among individuals with NAFLD.
The functional state of the liver, as indicated by

enzyme activity levels, revealed significant

differences between the groups. In the NAFLD
group, both alanine aminotransferase (ALT) and

gamma-glutamyl transferase (GGT) levels were

notably higher, with ALT levels at 43.2 ± 3.22 U/L
and GGT levels at 51.9 ± 11.6 U/L, compared to 24.4

± 1.82 U/L and 26.6 ± 2.72 U/L, respectively, in the
control group (p = 0.001 for ALT and p = 0.018 for

GGT). Additionally, aspartate aminotransferase
(AST) levels were higher in the NAFLD group (29.9

± 2.12) compared to the control group (23.3 ±
1.62). The AST/ALT ratio (De Ritis ratio) was lower

in NAFLD patients, averaging 0.83 ± 0.09, versus
1.06 ± 0.07 in the control group (p = 0.031),

suggesting liver health disparities between the two

groups.
Furthermore, the presence of early atherosclerosis,

as indicated by the thickness of the intima-media
complex of the common carotid artery (CCA IMT),

differed significantly between groups. Local
thickening of the CCA IMT exceeding 1.0 mm was

observed in 25% of the NAFLD group compared to
only 1.8% in the control group (p < 0.001). There

was also a direct correlation between the increase
in the oblique vertical size (OVS) of the liver and the

maximum value of the CCA IMT (R = 0.328, p =
0.001).
To further dissect the role of clinical or laboratory

risk factors associated with insulin resistance in

the onset of early atherosclerosis, evidenced by
changes in the CCA IMT, a stepwise multiple

regression analysis was conducted across the
entire patient cohort. This analysis included

variables that could plausibly link NAFLD with the
development of atherosclerotic damage to the

carotid arteries, aiming to build a mathematical
model that could elucidate these associations more

clearly.

Table No. 1

Descriptive statistics for dataset involving 100 patients analyzed through a stepwise regression

model focusing on the relationship between NAFLD and early atherosclerosis indicators:

Indicators included in the
model

Average value

Standard
deviation

Correlation coefficient K
with max CCA IMT

Max TKIM OSA, mm

0.8

0.2

BMI, kg/m2

24.7

3.3

0.4 (p < 0.001)

Age, years

40.0

7.0

0.5 (p < 0.001)

Liver CVR, mm

125.1

16.7

0.3 (p < 0.001)

OT, cm

85.9

12.0

0.5 (p < 0.001)

Triglycerides, mmol/l

1.2

1.0

0.4 (p < 0.001)

NAFLD*

0.4

0.5

0.5 (p < 0.001)

LDL cholesterol, mmol/l

3.5

0.1

0.3 (p = 0.002)

HDL cholesterol, mmol/l

1.4

0.3

0.2 (p = 0.005)

Fasting insulin, µIU /ml

7.7

4.4

0.5 (p < 0.001)

Fasting glucose, mmol/l

5.2

0.6

0.29 (p = 0.002)

Note: * The indicator had a value of 1 if the characteristic was present, and 0 if it was absent.

The observation that the severity of atherosclerotic

damage to the carotid arteries is most pronounced

when components of insulin resistance syndrome

are present together underscores the complex
interplay between metabolic disturbances and


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cardiovascular health. This finding, likely derived
from the analysis of the studied group, suggests

that the aggregation of insulin resistance syndrome

factors

such as hyperglycemia, dyslipidemia,

hypertension, and obesity

significantly elevates

the risk of developing atherosclerosis, especially in
the carotid arteries.
In the context of such a study, Table 2 would

presumably detail the extent of atherosclerotic
damage in correlation with various combinations

of insulin resistance syndrome components. This
table might include data on the prevalence of

carotid artery atherosclerosis, measured through

indicators such as the intima-media thickness
(IMT) and the presence of atherosclerotic plaques,

categorized by the presence or absence of specific

insulin resistance components. Additionally, it
could show statistical analyses, such as mean

values, standard deviations, and p-values,

indicating the significance of differences between
groups with different combinations of metabolic

risk factors.
The presence of such pronounced atherosclerotic

damage in individuals with multiple components of

insulin resistance syndrome highlights the
importance of a comprehensive approach to the

management of metabolic disorders. It emphasizes
the need for early detection and intervention to

mitigate the risk of cardiovascular diseases,

suggesting that treating insulin resistance and its
associated conditions could have a beneficial

impact on reducing the burden of atherosclerosis.

Table No. 2

Stepwise multiple regression analysis aimed at predicting early manifestations of

atherosclerotic lesions of the carotid arteries, 100 participants.

Predictors of Early Atherosclerotic Lesions in Carotid Arteries

PredictorVariable

B Coefficient

Standard

Error

BetaCoefficient

t-Statistic

p-Value

Fasting Blood Glucose

(mmol/L)

0.45

0.12

0.38

3.75

<0.001

Total Cholesterol

(mmol/L)

0.32

0.08

0.29

4.00

<0.001

Triglycerides (mmol/L)

0.27

0.09

0.25

3.00

0.003

LDL Cholesterol

(mmol/L)

0.22

0.10

0.20

2.20

0.029

HDL Cholesterol

(mmol/L)

-0.15

0.11

-0.13

-1.36

0.175

Body Mass Index

(kg/m^2)

0.10

0.05

0.18

2.00

0.047

NAFLD

0.073

0.03

0.29

2.10

<0.001

AlanineAminotransferase

(U/L)

0.05

0.02

0.15

2.50

0.013

Age (years)

0.01

0.03

0.02

0.33

0.740

The analysis concluded with the identification of a

prioritized list of prognostic factors that

significantly affect the alteration in the carotid
artery wall among participants of the study. This

ranking, in order of decreasing importance, places
LDL cholesterol at the top, followed by the

presence of non-alcoholic fatty liver disease

(NAFLD), and then the Homeostatic Model

Assessment for Insulin Resistance (HOMA-IR)
index. These findings underscore the critical role

these factors play in influencing the development
and progression of atherosclerosis, specifically

through changes in the intima-media thickness
(CCA IMT) of the common carotid artery.


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Despite the strong correlation observed between

triglyceride (TG) levels and changes in the CCA IMT

(R = 0.42, p < 0.001), this particular indicator was

excluded from the final regression model. This
exclusion suggests that while TG levels are

associated with carotid artery changes, their
impact might be mediated through or

overshadowed by other factors in the model, such
as LDL cholesterol, NAFLD, and insulin resistance,

as measured by the HOMA-IR index.
This hierarchy of prognostic factors highlights the

multifaceted nature of atherosclerosis, where lipid

profiles, liver health, and insulin resistance interact

in complex ways to influence cardiovascular risk.
The prominence of LDL cholesterol as the leading

factor reiterates its well-established role in
atherogenesis. Similarly, the inclusion of NAFLD

and HOMA-IR index points to the growing
recognition of liver health and metabolic

dysfunction as critical components in the
pathophysiology of atherosclerosis, further

emphasizing the need for a comprehensive
approach to cardiovascular risk assessment and

management.
The findings from the study clearly demonstrate a

link between non-alcoholic fatty liver disease
(NAFLD)

and

the

early

indications

of

atherosclerosis, underscoring the interconnected
nature of liver health and cardiovascular disease.

Specifically, the elevation in atherogenic lipids in
the blood profile not only predisposes individuals

to fatty infiltration of the liver but also plays a
pivotal role in exacerbating metabolic imbalances

within

the

div,

particularly

affecting

carbohydrate and lipid metabolism.
This relationship suggests that NAFLD does more

than just reflect underlying metabolic issues; it

actively contributes to the cascade of changes
leading to the worsening of these metabolic

disturbances. Consequently, NAFLD emerges as a
critical marker for the early detection of

atherosclerosis, highlighting its potential role as a

predictor for cardiovascular disease. The study’s

findings advocate for the inclusion of NAFLD in the
spectrum of factors considered during the

assessment of a patient’s car

diovascular risk

profile.
The progression from NAFLD to disturbances in

metabolism and then to atherosclerosis indicates a
pathophysiological pathway where liver health

significantly impacts overall metabolic health and
cardiovascular risk. This underscores the

importance of monitoring and managing NAFLD
not only as a liver-specific condition but also as a

component of systemic health strategies aimed at
preventing

atherosclerosis

and

related

cardiovascular diseases.

DISCUSSION

The research underscores the pivotal role of the

liver, particularly when affected by fatty hepatosis,
in the initiation and progression of metabolic

disturbances, specifically in carbohydrate and lipid
metabolism. It posits that the atherogenic impact of

non-alcoholic fatty liver disease (NAFLD) is largely

due to intracellular processes within hepatocytes.
These include increased lipid peroxidation leading

sequentially to heightened synthesis of highly
atherogenic substances such as triglycerides (TG)

and low-density lipoprotein cholesterol (LDL-C).
1.

This intricate mechanism underscores the

liver's central role in systemic metabolic regulation

and its contribution to cardiovascular risk through
the promotion of atherogenic lipid profiles. The

study's ability to rank prognostic factors that

influence changes in the carotid artery wall
presents a novel approach to evaluating the risk of

atherosclerosis in individuals who are clinically
healthy but may have underlying NAFLD.
2.

The clinical implications of these findings

are profound. They call for a more comprehensive
examination strategy for patients diagnosed with

NAFLD, emphasizing the need to look beyond liver
pathology alone. This strategy should include an

assessment of cardiovascular disease risks,

reflecting the intertwined nature of liver health and
heart disease. By identifying individuals at higher

risk for atherosclerosis early, based on the
presence of NAFLD and related metabolic

disturbances, healthcare providers can implement
preventive measures and interventions aimed at

mitigating the progression of both liver and


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(ISSN

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cardiovascular diseases.
3.

The identification and management of

NAFLD as a significant factor in cardiovascular risk
assessment reinforce the need for an integrated

approach to patient care, highlighting the
importance of cross-disciplinary collaboration in

the management of patients with metabolic
syndrome components. This comprehensive

assessment strategy aims not only to address the
hepatic manifestations of NAFLD but also to

proactively manage the associated increased risk of
cardiovascular disease, offering a pathway to more

effective prevention and treatment protocols.

REFERENCES
1.

Bacon, B. R., &Farahvash, M. J. (1994).

Nonalcoholic steatohepatitis: An expanded
clinical entity. Gastroenterology, 107, 1103

1109.

2.

Browning, J. D., Szczepaniak, L. S., Dobbins, R.,

et al. (2004). Prevalence of hepatic steatosis in
an urban population in the United States:

Impact of ethnicity. Hepatology, 40(1), 1387

1395.

3.

De Alwis, N. M., & Day, C. P. (2008). Non-

alcoholic fatty liver disease: The mist gradually

clears. Journal of Hepatology, 48(Suppl. 1),
104

112.

4.

Drapkina, O. M., Deeva, T. A., &Ivashkin, V. T.

(2015). Assessment of degree of endothelial
function and apoptosis in patients with

metabolic syndrome and nonalcoholic fatty
liver disease. TerapevticheskiiArkhiv, 87(5),

7683.

5.

Korneeva, O. N., Drapkina, O. M., Bueverov, S. A.,

&Ivashkin, V. T. (2005). Nonalcoholic fatty liver
disease as a manifestation of the metabolic

syndrome.
KlinicheskiePerspektivyGastroenterologiiiGep

atologii.

6.

Ivashkin, V. T., Drapkina, O. M., Maiev, I. V., et al.

(2015). The prevalence of nonalcoholic fatty
liver disease in patients of outpatient practice

in the Russian Federation: Results of the DIREG
2 trial. RossiyskiyZhurnalGastroenterologii,

Gepatologii, Koloproktologii, 6, 31

41.

7.

Roden, M. (2006). Mechanisms of disease:

Hepatic steatosis in type 2 diabetes -
Pathogenesis and clinical relevance. Nature

Clinical Practice Endocrinology & Metabolism,
2(6), 335

348.

8.

Perez-Aguilar, F. (2005). Etiopathogenesis of

non-alcoholic

steatohepatitis.

Gastroenterologia y Hepatologia, 28(7), 396

406.

9.

Lonardo, A. (2005). Review article: Hepatic

steatosis and insulin resistance. Alimentary
Pharmacology & Therapeutics, 22(2), 64

70.

10.

Lazebnik, B. L., Zvenigorodskaia, L. A.,

&Egorova, E. G. (2005). Metabolic syndrome

from the gastroenterologist's perspective. RMJ,
13(26), 1706

1712. [In Russian]

11.

Leon, A. A., Paul, A., & Lindor, K. D. (2005).

Nonalcoholic fatty liver disease. CMAJ, 29, 172

178.

12.

Chalasani, N., Younossi, Z., Lavine, J. E., et al.

(2012). The diagnosis and management of non-

alcoholic fatty liver disease: Practice guideline
by the American Association for the Study of

Liver

Diseases,

American

College

of

Gastroenterology,

and

the

American

Gastroenterological Association. Hepatology,
55, 2005

2023.

13.

Salvi, P., Ruffini, R., Agnoletti, D., et al. (2010).

Increased arterial stiffness in nonalcoholic

fatty liver disease: The Cardio-GOOSE study.
Journal of Hypertension, 28(8), 1699

1707.

14.

Ramilli, S., Pretolani, S., Muscari, A., et al.

(2009). Carotid lesions in outpatients with
nonalcoholic fatty liver disease. World Journal

of Gastroenterology, 15(38), 4770

4774.

15.

Yoneda, M., Mawatari, H., Fujita, K., et al.

(2007). High-sensitivity C-reactive protein is
an independent clinical feature of non-

alcoholic steatohepatitis (NASH) and also of the
severity of fibrosis in NASH. Journal of

Gastroenterology, 42, 573

582.

16.

Drapkina, O. M., Bueverova, E. L., &Ivashkin, V.


background image

THE USA JOURNALS

THE AMERICAN JOURNAL OF MEDICAL SCIENCES AND PHARMACEUTICAL RESEARCH
(ISSN

2689-1026)

VOLUME 06 ISSUE06

17

https://www.theamericanjournals.com/index.php/tajmspr

T. (2010). Atherogenic dyslipidemia and liver.
AteroskleroziDislipidemii, 1, 25

30.

17.

Ivashkin, V. T. (2010). Nuclear receptors and

liver

disease.

Part

2-I.

RossiyskiyZhurnalGastroenterologii,
Gepatologii, Koloproktologii, 4, 7

15.

18.

Korneeva, O. N., &Drapkina, O. M. (2012). How

to avoid the appointment of statin

hepatotoxicity in patients with obesity and
related liver disease? Focus on a combination

of ursodeoxycholic acid and atorvastatin.
KardiovaskulyarnayaTerapiyaiProfilaktika,

11(6), 81

84.

19.

Martsevich, S. Y., Kutishenko, N. P., Drozdova, L.

Y., et al. (2014). Study of ursodeoxycholic acid

influence on efficacy and safety of statin
therapy in patients with diseases of the liver,

gall bladder and/or biliary tract (the RAKURS

study).

Rational

Pharmacotherapy

in

Cardiology, 10(2), 147

152.

20.

Kim, B. J., Kim, N. H., Kim, B. S., & Kang, J. H.

(2012). The association between nonalcoholic
fatty liver disease, metabolic syndrome, and

arterial

stiffness

in

nondiabetic,

nonhypertensive individuals. Cardiology, 123,

54

61.

21.

Lee, Y. J., Shim, J. Y., Moon, B. S., et al. (2012).

The relationship between arterial stiffness and
nonalcoholic fatty liver disease. Digestive

Diseases and Sciences, 57, 196

203.

22.

Zyatenkova, E. V., Drapkina, O. M., &Ivashkin, V.

(2014). Characteristics of vessels wall,

myocardium, and epicardial fat in patients with

heart failure with preserved ejection fraction
with and without metabolic syndrome.

Endoscopic Ultrasound, 3(Suppl 1), S1

S2.

References

Bacon, B. R., &Farahvash, M. J. (1994). Nonalcoholic steatohepatitis: An expanded clinical entity. Gastroenterology, 107, 1103–1109.

Browning, J. D., Szczepaniak, L. S., Dobbins, R., et al. (2004). Prevalence of hepatic steatosis in an urban population in the United States: Impact of ethnicity. Hepatology, 40(1), 1387–1395.

De Alwis, N. M., & Day, C. P. (2008). Non-alcoholic fatty liver disease: The mist gradually clears. Journal of Hepatology, 48(Suppl. 1), 104–112.

Drapkina, O. M., Deeva, T. A., &Ivashkin, V. T. (2015). Assessment of degree of endothelial function and apoptosis in patients with metabolic syndrome and nonalcoholic fatty liver disease. TerapevticheskiiArkhiv, 87(5), 7683.

Korneeva, O. N., Drapkina, O. M., Bueverov, S. A., &Ivashkin, V. T. (2005). Nonalcoholic fatty liver disease as a manifestation of the metabolic syndrome. KlinicheskiePerspektivyGastroenterologiiiGepatologii.

Ivashkin, V. T., Drapkina, O. M., Maiev, I. V., et al. (2015). The prevalence of nonalcoholic fatty liver disease in patients of outpatient practice in the Russian Federation: Results of the DIREG 2 trial. RossiyskiyZhurnalGastroenterologii, Gepatologii, Koloproktologii, 6, 31–41.

Roden, M. (2006). Mechanisms of disease: Hepatic steatosis in type 2 diabetes - Pathogenesis and clinical relevance. Nature Clinical Practice Endocrinology & Metabolism, 2(6), 335–348.

Perez-Aguilar, F. (2005). Etiopathogenesis of non-alcoholic steatohepatitis. Gastroenterologia y Hepatologia, 28(7), 396–406.

Lonardo, A. (2005). Review article: Hepatic steatosis and insulin resistance. Alimentary Pharmacology & Therapeutics, 22(2), 64–70.

Lazebnik, B. L., Zvenigorodskaia, L. A., &Egorova, E. G. (2005). Metabolic syndrome from the gastroenterologist's perspective. RMJ, 13(26), 1706–1712. [In Russian]

Leon, A. A., Paul, A., & Lindor, K. D. (2005). Nonalcoholic fatty liver disease. CMAJ, 29, 172–178.

Chalasani, N., Younossi, Z., Lavine, J. E., et al. (2012). The diagnosis and management of non-alcoholic fatty liver disease: Practice guideline by the American Association for the Study of Liver Diseases, American College of Gastroenterology, and the American Gastroenterological Association. Hepatology, 55, 2005–2023.

Salvi, P., Ruffini, R., Agnoletti, D., et al. (2010). Increased arterial stiffness in nonalcoholic fatty liver disease: The Cardio-GOOSE study. Journal of Hypertension, 28(8), 1699–1707.

Ramilli, S., Pretolani, S., Muscari, A., et al. (2009). Carotid lesions in outpatients with nonalcoholic fatty liver disease. World Journal of Gastroenterology, 15(38), 4770–4774.

Yoneda, M., Mawatari, H., Fujita, K., et al. (2007). High-sensitivity C-reactive protein is an independent clinical feature of non-alcoholic steatohepatitis (NASH) and also of the severity of fibrosis in NASH. Journal of Gastroenterology, 42, 573–582.

Drapkina, O. M., Bueverova, E. L., &Ivashkin, V. T. (2010). Atherogenic dyslipidemia and liver. AteroskleroziDislipidemii, 1, 25–30.

Ivashkin, V. T. (2010). Nuclear receptors and liver disease. Part 2-I. RossiyskiyZhurnalGastroenterologii, Gepatologii, Koloproktologii, 4, 7–15.

Korneeva, O. N., &Drapkina, O. M. (2012). How to avoid the appointment of statin hepatotoxicity in patients with obesity and related liver disease? Focus on a combination of ursodeoxycholic acid and atorvastatin. KardiovaskulyarnayaTerapiyaiProfilaktika, 11(6), 81–84.

Martsevich, S. Y., Kutishenko, N. P., Drozdova, L. Y., et al. (2014). Study of ursodeoxycholic acid influence on efficacy and safety of statin therapy in patients with diseases of the liver, gall bladder and/or biliary tract (the RAKURS study). Rational Pharmacotherapy in Cardiology, 10(2), 147–152.

Kim, B. J., Kim, N. H., Kim, B. S., & Kang, J. H. (2012). The association between nonalcoholic fatty liver disease, metabolic syndrome, and arterial stiffness in nondiabetic, nonhypertensive individuals. Cardiology, 123, 54–61.

Lee, Y. J., Shim, J. Y., Moon, B. S., et al. (2012). The relationship between arterial stiffness and nonalcoholic fatty liver disease. Digestive Diseases and Sciences, 57, 196–203.

Zyatenkova, E. V., Drapkina, O. M., &Ivashkin, V. (2014). Characteristics of vessels wall, myocardium, and epicardial fat in patients with heart failure with preserved ejection fraction with and without metabolic syndrome. Endoscopic Ultrasound, 3(Suppl 1), S1–S2.