This article presents the results of a study on the determination of polyphenols in Black tea by high-performance liquid chromatography (HPLC). Polyphenols are bioactive compounds in plants, whose antioxidant properties have a therapeutic effect against various diseases. During the study, the main polyphenols (e.g. flavonoids and phenolic acids) in Black tea were identified and their quantitative indicators were determined. The effective effect of these compounds on eye diseases, in particular, age-related macular degeneration, glaucoma, and dry eye syndrome, was studied. The results showed that polyphenols in Karachay play an important role in protecting eye tissues from oxidative stress and reducing inflammatory processes. The results of this study serve to increase the pharmacological value of Karachay and expand the possibilities of its application in the healthcare sector.