Volume 02 Issue 04-2022
61
American Journal Of Applied Science And Technology
(ISSN
–
2771-2745)
VOLUME
02
I
SSUE
04
Pages:
61-67
SJIF
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MPACT
FACTOR
(2022:
6.
108
)
OCLC
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1121105677
METADATA
IF
–
5.582
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ABSTRACT
In medical practice, for the prevention and treatment of various diseases, anti-inflammatory, antimicrobial and
regenerative herbal medicines are widely used. The healing properties of medicinal plants are due to active or
pharmacologically active substances - alkaloids, glycosides, saponins, tannins, enzymes, vitamins, hormones,
phytoncides, etc. They are the most valuable, although they are contained in plants in minimal quantities. The article
presents the results of obtaining a bactericidal, wound-healing ointment based on a dry extract of celandine herb,
propolis and sea buckthorn oil.
KEYWORDS
Dry extract, celandine herb, technology, ointment, propolis, sea buckthorn oil.
Research Article
NEW WAY SCOPING OF TECHNOLOGY FOR OBTAINING BACTERICIDAL
AND WOUNDING OINTMENT
Submission Date:
April 18, 2022,
Accepted Date:
April 25, 2022,
Published Date:
April 30, 2022
Crossref doi:
https://doi.org/10.37547/ajast/Volume02Issue04-09
N.B. Abdunazarova
Trainee At A. Sultanov Chemical-Pharmaceutical Research Institute, Uzbekistan
V.R. Khaydarov
Candidate Of Pharmaceutical Sciences, Professor, Head Of The Department Of Industrial Technology Of
Pharmaceuticals, Pharmaceutical Institute, Tashkent, Uzbekistan
G.B. Vafakulova
Director Of The Research Institute «Sharq Tabobati», Uzbekistan
U.A. Khadzhieva
Head Of The Laboratory At A. Sultanov Chemical-Pharmaceutical Research Institute, Uzbekistan
Journal
Website:
https://theusajournals.c
om/index.php/ajast
Copyright:
Original
content from this work
may be used under the
terms of the creative
commons
attributes
4.0 licence.
Volume 02 Issue 04-2022
62
American Journal Of Applied Science And Technology
(ISSN
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2771-2745)
VOLUME
02
I
SSUE
04
Pages:
61-67
SJIF
I
MPACT
FACTOR
(2022:
6.
108
)
OCLC
–
1121105677
METADATA
IF
–
5.582
Publisher:
Oscar Publishing Services
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INTRODUCTION
Herbal medicinal products, due to their versatile
activity, low toxicity and good digestibility, occupy an
important place in world practical medicine. At the
same time, special attention should be paid to the use
of accessible, cultivated and natural sources of raw
materials, promising in terms of implementation on
the basis of created technologies for new
preparations from them.
One of these plants is celandine herb, which has long
been used in medical practice and has versatile
pharmacological activity: antimicrobial, antiviral, anti-
inflammatory,
immunomodulatory,
choleretic,
antispasmodic,
antitumor,
analgesic,
which
determines the prospects for further study and
development of new drugs based on it [1 -2]. In
medicine, celandine is used in the form of various
dosage forms: water extracts, tinctures, juice from a
fresh plant, ointments, suppositories.
The main active ingredients of celandine are a
complex of alkaloids, which is represented by
compounds with different chemical structures and
pharmacological
activity.
Quantitatively,
benzphenanthridines
chelidonine
(0.3-1.0%),
homohelidonine (0.3-4%), sanguinarine (0.2 0.4%) and
chelerythrine (0.1-0.3%) prevail, in smaller quantities
contain
protopins-protopin
(0.2-0.4%)
and
allokryptopin (0.1%) and protoberberines - berberine
(0.1-0.3%), coptisine (0.2%), etc.
At A. Sultanov Chemical-Pharmaceutical Research
Institute, a technology for obtaining a dry extract of
celandine herb was developed. To obtain a dry extract
of celandine herb, celandine herb presented by the
Research Institute "Eastern Medicine" was used.
Methods have been developed to obtain a dry extract
from celandine herb. The obtained dry extract of
celandine herb was standardized according to the
requirements of the State Pharmacopoeia XI and
according to the VFS project for a dry extract of
celandine herb.
Dry extracts obtained from medicinal plants are the
basis for creating a new drug.
Currently, combined ointments are used to enhance
the therapeutic efficacy and expand the action.
Known
anti-inflammatory
and
wound-healing
ointment containing oil extract of plant components,
as well as honey, mummy, propolis and bear fat [3].
This tool requires long-term use and is not used in the
treatment of colpitis, in addition, it contains a scarce
raw material - bear fat.
The aim of this work is the development of a
bactericidal, wound-healing ointment with a wide
spectrum of therapeutic action based on a
combination of a dry extract of celandine herb, sea
buckthorn oil and propolis.
Sea buckthorn oil, obtained from sea buckthorn fruits
by extraction with vegetable oil. It has an analgesic,
anti-inflammatory,
antibacterial
effect,
is
a
multivitamin and antitumor agent, accelerates the
granulation of epithelialization of tissues (with
frostbite, thermal, chemical and radiation burns,
gynecological inflammatory diseases, colpitis, vitamin
deficiency,
hemorrhoids,
pulpitis,
periodontal
disease). The anti-burn and repair activity of the oil is
due to the presence of β-sitosterol and other
triterpenoids.
Propolis is very rich in biologically active substances.
The amount of phenolic compounds is not less than
25.0%. Propolis has analgesic properties, therefore it is
widely used in apitherapeutic treatment in various
Volume 02 Issue 04-2022
63
American Journal Of Applied Science And Technology
(ISSN
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2771-2745)
VOLUME
02
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04
Pages:
61-67
SJIF
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FACTOR
(2022:
6.
108
)
OCLC
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1121105677
METADATA
IF
–
5.582
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forms, both in pure form and in preparations (tablets,
suppositories, ointments, etc.).
Experimental part.
Getting a dry extract of celandine herb. Crushed dry
herb of celandine in an amount of 300 g is loaded into
a container equipped with a stirrer and ethyl alcohol
70% in an amount of 6000 ml is added. The extraction
is carried out at a temperature of 55 ° C for 8 hours.
The resulting extract is separated from the meal by
filtration and then concentrated at a temperature of
60 ° C and a pressure of 0.6 atm. up to a volume of
4300 ml. The thickened extract is poured into a baking
sheet and dried in a vacuum drying oven at a
temperature of 70 ° C to dryness, which is then ground
to a particle size of 0.5 mm and sieved. The yield of
dry extract is 70 g (23.3%). Dry extract of celandine
herb is a dark brown powder with a characteristic
odor.
To obtain an ointment, an emulsion base is first
prepared. To do this, in a mortar heated in a water
bath, melt 60 g of petroleum jelly, 10 g of emulsifier T-
2 and add 30 ml of purified water, mix until
homogeneous, get an emulsion base - oil / water.
Concentration of propolis tincture. In a porcelain cup,
heated, in a water bath, load 40 ml of 5% propolis
tincture, which is evaporated at a temperature of 78 °
C to 20 ml, which corresponds to 2 g of pure propolis.
Getting the ointment. To 96 g of the emulsion base,
with vigorous stirring, add 2 g of dry extract of
celandine herb, 2 g of sea buckthorn oil, 20 ml of
concentrated propolis solution and continue stirring
for 10 minutes with the addition of the rest of the
emulsion base until a creamy mass is formed. The
output of the ointment is 100 g. The technological
scheme for obtaining the ointment is shown in Fig. 1.
The quality of the ointment was assessed according to
the following indicators: appearance, homogeneity,
authenticity, pH of the aqueous extract of the
ointment, physicochemical stability and the norm of
permissible deviations in the mass of the ointment.
The prepared ointment is a homogeneous mass of
mustard color with a specific odor.
Determination of the homogeneity of the ointment
was carried out according to the method of GF XI. The
ointment should be uniform. To do this, take 4
samples of ointment, 0.02-0.03 g each, placing them
in 2 samples on a glass slide, covered with a slide on
top, pressing tightly until spots with a diameter of 2
cm are formed. When examining the spots with the
naked eye (at a distance of 30 cm) 3 out of 4 samples
should be free of visible particles. If it is found in a
larger number of spots, then the determination is
repeated on 8 samples. In this case, the presence of
visible particles is allowed no more than in 2 spots.
The studied ointment is homogeneous; no visible
particles were observed in it.
The determination of the pH of the aqueous solution
of the ointment was carried out potentiometrically.
To do this, first, 5.0 g of the ointment was mixed with
50 ml of purified water, heated in a boiling water bath
for 5 minutes with stirring. Cooled and filtered
through cotton wool. The pH value of the ointment is
in the range of 6.0 to 7.0.
Physico-colloidal stability of ointments is determined
primarily by the combination of its kinetic,
aggregative and condensation stability. Ointments
during storage should not delaminate, change their
consistency, and release solid particles of suspended
medicinal substances. It is important that the
ointments are stable at temperature fluctuations (as a
rule, at temperatures from -40 0 to +40 0С).
Volume 02 Issue 04-2022
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American Journal Of Applied Science And Technology
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VOLUME
02
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108
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1121105677
METADATA
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–
5.582
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1
Staff training
Des.
solutions,
Н2О2, Н2О,
steam,
detergents
2
Preparation of
industrial
premises
Petrolatum
3
Equipment
training
Down the drain
4.1
Melting and
filtering
petroleum
4
Preparation of
raw materials
Mechanical
losses
Volume 02 Issue 04-2022
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VOLUME
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jelly
Celandine
substance.
Water
Petrolatum
Emulsifier
Sea buckthorn
oil
Solution
celandine
4.2
Dissolving
celandine
4.3
Concentratio
n of propolis
tincture
1
Getting ointment
Mechanical
losses
1.1
Mixing
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VOLUME
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Banks
1
Packing and
packaging of
ointment
Mechanical
losses
1.1
Packaging
1.2
Рacking
To the
warehouse of
finished
products
Fig. 1. Technological scheme for obtaining ointment
When obtaining compositions that meet their
structural and mechanical characteristics, it is
necessary to investigate the ability of the ointment to
sediment solid particles, release a liquid phase. For
this, the methods of centrifugation and thermostating
of the ointment are used with changing temperature
fluctuations.
5.0 g of the ointment was placed in a TsUM-1
centrifuge and centrifuged at 1.5 thousand rpm. No
Volume 02 Issue 04-2022
67
American Journal Of Applied Science And Technology
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2771-2745)
VOLUME
02
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SSUE
04
Pages:
61-67
SJIF
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(2022:
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108
)
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–
1121105677
METADATA
IF
–
5.582
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separation of the liquid phase was observed,
therefore, the studied ointment showed its stability
under experimental conditions.
To study physicochemical stability by thermostatting,
ointment samples weighing 20.0 g were placed in
weighing bottles 35 mm in diameter and kept at a
temperature of 40 ± 0.2 ° C for 6 hours in a TC-M
thermostat until possible destruction. Then, after
cooling, it was centrifuged in a TsUM-1 centrifuge for 5
min at a rotor speed of 1.5 thous.
The indicator that determines the stability of the
ointment during storage is the volatility of the liquid
phase. 1.0 g of ointment (accurately weighed) was
placed in a pre-dried and weighed weighing bottle 36
mm in diameter and 62 mm in height and heated in a
boiling water bath for 1 h. Then it was placed in an
extractor for cooling. Weight loss should be no more
than 10%. The norm of permissible deviations for
ointments is from 20.0 to 30.0 ± 7%.
The obtained ointment is satisfactory in physical,
chemical and technological properties and meets the
requirements for ointments.
The resulting ointment was transferred to the
Research Institute "Eastern Medicine" for preclinical
pharmacological studies.
Preclinical pharmacological study of wound healing
ointment was carried out by creating a model of
thermal burn of IIIA degree and the creation of an
experimental model of colpitis was carried out
according to the improved methods of D.S. Sarkisov.
and etc.
The research results showed that the proposed
ointment has new - removing necrotic tissue,
regenerating and restoring the mucous membrane
and accelerating the epithelialization processes,
which forms a film on the surface of wounds,
anesthetic,
antiseptic
and
anti-inflammatory
properties, in addition, it is successfully recommended
for the treatment of gynecological diseases.
Thus, a technology has been developed for obtaining
a bactericidal, wound-healing ointment.
On the basis of the data obtained, the VFS project was
developed and the laboratory and technological
regulations for the ointment were drawn up.
REFERENCES
1.
Turova A.D., Sapozhnikova E.N. / Medicinal plants
of the USSR and their application. - M., 1983.
2.
Volkina I.V., Gurevich I.I. // Prescription guide for
doctors and pharmacists. –L., Medicine, 1976.
3.
Patent RU 2246310.
